| Project/Area Number |
12440209
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
分離・精製・検出法
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| Research Institution | Nagoya Institute of Technology |
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Principal Investigator |
TSUDA Takao Nagoya Institute of Technology, Faculty of Engineering, Professor, 工学部, 教授 (50023156)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥10,400,000 (Direct Cost: ¥10,400,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2001: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2000: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | electrochromatography / variation of capacity factor / AV-CEC / frequency analysis / open tubular capillary column / electroosmotic flow / flow profile / 周波数特性解析 / 交流電場 / カラム電気抵抗 / オープンチューブカラム / 充填剤表面解析 / 保持比の変化 |
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| Research Abstract |
The objective of our research was the study and application of voltage-induced variation of properties of stationary phase in capillary electrochromatography. We could achieve following results in our research.
1. Study of Voltage-Induced Capacity Factor Variation
The voltage-induced capacity factor variations of neutral solute were investigated using electrochromatography with stationary phases of β-CD and mixed-mode (ODS-SAX). We found that the applied electric field can control a surface charge density of the packed materials, and it induced the variation of the capacity factor.
2. Development of Alternative Voltage Capillary Electrochromatography (AV-CEC)
The new separation mode for capillary electrochromatography, i.e.Alternative Voltage Capillary Electrochromatography (AV-CEC), was developed. In AV-CEC, the alternating voltage was applied to control of the retention behavior of sample solute. We found that the retention behavior was strongly affected a frequency and an amplitude of an applied alternating voltage.
3. Development of high performance open tubular capillary column
The new open-tubular capillary column with modified silica-gel thin layer for capillary electrochromatography was developed. Our new open tubular column of a few cm lengths could perform high separation efficiency.
4. Study of electroosmotic flow
Electroosmotic flow, which is one of the basic factor of electrochromatography, was investigated using frequency-domain analysis method of Fourier transform. Since an electroosmotic flow was strongly affected by a surface property of the packing materials, we developed the method to measure the surface property using an electroosmotic flow velocity. Information about the dissociation constant of a functional group on the surface and its density were measured.
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