| Project/Area Number |
12440224
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | Tokyo University of Agriculture and Technology, Faculty of Technology |
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Principal Investigator |
QZEKI Yoshlhiro Tokyo University of Agriculture and Technology, Faculty of Technology, Associate Professor, 工学部, 助教授 (50185592)
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| Co-Investigator(Kenkyū-buntansha) |
MIMURA Tetsuro Nara Women's University, Faculty of Science, Professor, 理学部, 教授 (20174120)
YAMADA Akiyo Tokyo University of Agriculture and Technology, Faculty of Technology, Assitant Professor, 工学部, 助手 (30293012)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 2001: ¥4,300,000 (Direct Cost: ¥4,300,000)
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| Keywords | mangrove / halophyte / sail tolerance / environmental stress tolerance / mangrin |
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| Research Abstract |
Functional screening of cDNAs encoding proteins essential for the stress tolerance in mangrove plants was performed using Escherichia coli as a host organism. Twenty-nine E. coli transformants, which showed remarkable growth under salt-stress conditions, were obtained from 1,000,000 E. coli transformants. Based on the sequence analyzes from the screened transformants, twenty-three clones have an identical nucleotide sequence. A full-length CDNA is 1018 bp and the ORF encodes a protein of 141 amino acids including 28 serines (19.86%). It is revealed that there are no similar proteins of all other entries in databases. We designated this protein to "mangrin". In order to test the effect of mangrin expression on salt-tolerance in eucaryotic cells, mangrin CDNA ligated with GAL1 and 35S promoter were introduced to yeast and tobacco cell culture, respectively. As well as E. coli experiment, the growth rates of these cultures expressing mangrin were enhanced as compared to the control strains. It is possible that mangrin may function to give salt-tolerance to diverse organisms at cellular level. In order to test the effect of mangrin expression on the salt-tolerance in higher plant at organ level, mangrin CDNA driven by 35S promoter was introduced to tobacco plants. Growth of control transformants was strongly inhibited by addition of 150 mM NaCl to the medium. In contrast, transformants expressing mangrin showed remarkable well growth as compared to the controls in medium containing 150 mM NaCl. Southern blot analysis on various plant species using a mangrin CDNA probe indicated that other plant species have not mangrin or its homologue. Mangrin may specially exist in mangrove plants. Discovery of mangrin will contribute to elucidate salt-tolerance mechanisms in mangrove plants at molecular biological level. Biosynthesis of mangrin will open a new window to enhance salt-tolerance of higher plants, including crop plants.
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