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Functional analysis of KN1-type homeodomain protein

Research Project

Project/Area Number 12440227
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionNagoya University

Principal Investigator

MATSUOKA Makoto  Bioscience Center Nagova University Professor, 生物分子応答研究センター, 教授 (00270992)

Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥12,300,000 (Direct Cost: ¥12,300,000)
Fiscal Year 2001: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2000: ¥7,300,000 (Direct Cost: ¥7,300,000)
Keywordshomeobox gene / rice / tobacco / gibberellin (GA) / transgenic plant / 形質転換植物 / ホメオドメイン / ジベレリン / 転写制御
Research Abstract

KNOX horaeodomain proteins are encoded by knottedl-like homeobox (knox) genes that constitute a gene family in plants. Similar to the animal homeodomain proteins, KNOX proteins are consider ed to be key transcriptional regulators that control the expression of genes involved in plant organogenesis at the shoot apical meristemi Therefore, in order to understand the developmental processes in plants, it is important to elucidate the molecular mechanisms underlying KNOX pro tein regulation of gene expression. In thip study, we investigated the structural features and the mechanisms for expression of KNOX proteins. Through the experiments with transgenic plants, a KNOX protein, OSH15, directly interacts with the specific sequence of intron 1 in the gene e needing GA biosynthetic enzyme, GAC20 oxidase and suppresses its expression.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Kikuchi, K.: "Molecular analysis of the NAC gene family in rice"Mol. Gen. Genet.. 262. 1047-1051 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishimura, A.: "Over-expression of tobacco knotted1 class1 homeobox genes alters various leaf morphology"Plant Cell Physiol.. 41. 583-590 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Itoh, J.-I.: "SHOOT ORGANIZATION genes regulate shoot apical meristem organization and the pattern of leaf primordium initiation in rice"Plant Cell. 12. 2161-2174 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakamoto, T.: "KNOX homeodomain protein directly suppresses the expression of a gibberellin biosynthetic gene in the tobacco shoot apical meristem"Genes and Development. 15. 581-590 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakamoto, T.: "Function of KNOX homeodomain proteins in pant development"Plant Biotech.. 18. 85-92 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagasaki, H.: "Functional analysis of the conserved domains of a rice KNOX homeodomain protein, OSH 15"Plant Cell. 13. 2085-2098 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ito, M.: "Involvement of homeobox genes in early body plan of monocot"International review of cytology (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kikuchi, K: "Molecular analysis of the NAG gene family in rice"Mol. Gen. Genet. 262. 1047-1051 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishimura, A: "Over-expression of tobacco knottedl-type classl homeobox genes alters various leaf morphology"Plant Cell Physiol. 41. 583-590 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Itoh, J: "SHOOT ORGANIZATION genes regulate shoot apical meristem or ganization and the pattern of leaf primordium initiation in rice"Plant Cell. 12. 2161-2174 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakamoto, T: "KNOX homeodomain protein directly suppresses the expression of a gibberellin biosynthetic gene in the tobacco shoot apical meristem."Genes and Development. 15. 581-590 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakamoto, T: "Function of KNOX homeodomain proteins in pant development."Plant Biotech. 18. 85-92 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagasaki, H: "Functional analysis of the conserved domains of a rice KNOX homeodomain protein, OSH15"Plant Cell. 13. 2085-2098 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ito, M: "Involvement of homeobox genes in early body plan of monocot"International review of cytology. in press. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kikuchi, K.: "Molecular analysis of the NAC gene family in rice"Mol. Gen. Genet.. 262. 1047-1051 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishimura, A.: "Over-expression of tobacco knotted1 class1 homeobox genes alters various leaf morphology"Plant Cell Physiol.. 41. 583-590 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Itoh, J.-I.: "SHOOT ORGANIZATION genes regulate shoot apical meristem organization and the pattem of leaf primordium initiation in rice"Plant Cell. 12. 2161-2174 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sakamoto, T.: "KNOX homeodomain protein directly suppresses the expression of a gibberellin biosynthetic gene in the tobacco shoot apical meristem"Genes and Development. 15. 581-590 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sakamoto, T.: "Function of KNOX homeodomain proteins in pant development"Plant Biotech.. 18. 85-92 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nagasaki, H.: "Functional analysis of the conserved domains of a rice KNOX homeodomain protein, OSH15"Plant Cell. 13. 2085-2098 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ito, M.: "Involvement of homeobox genes in early body plan of monocot."International review of cytology(in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Kikuchi,K.,: "Molecular analysis of the NAC gene family in rice."Mol.Gen.Genet.. 262. 1047-1051 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nishimura,A.,: "Over-expression of tobacco Knotted 1-type class 1 homeobox genes afters various leaf morphology."Plant Cell Physiol.. 41. 583-590 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sentoku,N.,: "Over-expression of rice OSH genes induces ectopic shoots on leaf sheaths of transgenic rice plants."Develop.Biol.. 220. 358-364 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sakamoto,T.,: "KNOX homeodomain protein directly suppresses the expression of a gibberellin biosynthetic gene in the tobacco shoot apical meristem."Genes and Development. (in press). (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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