• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanisms of response to environmental stresses with active oxygen species-detoxification in higer plants

Research Project

Project/Area Number 12440229
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionUniversity of Shizuoka

Principal Investigator

KOBAYASHI Hirokazu  University of Shizuoka, Graduate School of Nutritional and Environmental Sciences, Associate Professor, 大学院・生活健康科学研究科, 助教授 (80170348)

Co-Investigator(Kenkyū-buntansha) NIWA Yasuo  University of Shizuoka, Graduate School of Nutritional and Environmental Sciences, Research Associate, 大学院・生活健康科学研究科, 助手 (00222191)
KAWAKITA Kazuhito  Nagoya University, Graduate School of Bioagricultural Sciences, Associate Professor, 大学院・生命農学研究科, 助教授 (90186065)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥15,200,000 (Direct Cost: ¥15,200,000)
Fiscal Year 2002: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2001: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 2000: ¥7,000,000 (Direct Cost: ¥7,000,000)
Keywordsenvironmental stress / response / salt tolerance / Arabidopsis / mutant / cDNA macroarray / oligo-microarray / alternative splicig / cDNAマイクロアレイ / Na^+ / H^+-アンチポーター / 細胞膜H^+-ATPase / DNAマクロアレイ / 活性酸素解毒系活性 / 光ストレス / プロリン
Research Abstract

Salt tolerance in plants has been attributed to many mechanisms, including accumulation of osmoregulatory solutes inside cells, enhanced ability of cells to take up water or K^+ from surrounding solutions of higher salt concentration, elimination of excess NaCl, and the ability to maintain photosynthetic activity under salt stress. To further investigate the mechanisms of salt tolerance, we have begun to screen and characterize mutants of Arabidopsis thaliana tolerant to high NaCl concentrations during photoautotrophic growth. Several mutants of A. thaliana with altered responses to salt have been reported, including mutants tolerant of salt during germination and mutants that are salt-hypersensitive. However, there have been no reports of mutants whose photoautotrophic growth is salt tolerant.
We selected pst (photoautotrohic salt tolerance) mutant lines of Arabidopsis, and characterized pst1 plants in which activities of active oxygen species-detoxifying enzymes were enhanced (Plant Cell, 11, 1195-1206, 1999). However, it was difficult to specify the event crucial for conferring salt tolerance on pst2 plants. The cDNA macroarray and oligo-microarray were performed for pst2 and wild-type lines grown under a non-stress condition. Higher expression of a transcription factor with bHLH motif in pst2 plants was detected, and further confirmed by real-time RT-PCR. There was another specie of mRNA produced by alternative splicing under the salt stress. The RACE has clarified that transcription initiation site is single. The salt-induced mRNA retained an intron on C-terminal side among two introns, and the newly generated stop codon of first ORF and its subsequent initiation codon of new second ORF may encode two separate peptide molecules which looked like cleaved forms of the original protein. Protein species derived from the bHLH gene are being analyzed by immuno-blotting, and the function of bHLH transcription factor is also examined with its transgenic plants.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] K.Uemura, N.Shibata, Anwaruzzaman, M.Fujiwara, T.Higuchi, H.Kobayashi, Y.Kai, A.Yokota: "The role of structural intersubunit microheterogeneity in the regulation of the activity in hysteresis of ribulose 1,5-bisphosphate carboxylase/oxygenase"J.Biochem.. 128. 591-599 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Isono, K.Satoh, H.Kobayashi: "Molecular cloning of a cDNA encoding a novel Ca^<2+>-dependent nuclease of Arabidopsis is that is similar to staphylococcal nuclease"Bioch.Biophys.Acta. 1491. 267-272 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Yoshimoto, M.Sakaiya, K.Isono, H.Kobayashi: "Comparison of strength of endogenous and exogenous gene promoters in Arabidopsis chloroplasts"Plant Biotechnol.. 18. 135-142 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 清水正則, 吉本光希, 小林裕和: "葉緑体の転写調節:光合成機能発現制御機構"蛋白質拡散酵素. 45. 123-131 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 小林裕和, 小林京子: "新しい高等植物耐塩機構:劣勢遺伝子支配"バイオサイエンスとインダストリー. 58. 489-490 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 加藤秀起, 小林裕和: "σ因子の多様性から見た葉緑体の機能発現"植物細胞工学シリーズ. 17. 114-122 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Kobayashi, H.Kato, M.Shimizu: "Symbiotic aspects of participation of multiple σfactor in functional chloroplasts(in"Symbiosis and Eukaryotic Organells"M.Sugiura, and J.Obokata, eds.)"Thuringen University Library, Thuringen(印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 小林裕和: "飢餓を救う植物遺伝子組み換え(「人間といのち---ヘルスサイエンス」静岡新聞社編)"静岡新聞社. 78-85 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 蘆田弘樹, 小林裕和, 赤澤 尭, 横田明穂: "リブロース-1,5-ビスリン酸カルボキシラーゼ/オキシゲナーゼ(EC4.1.1.39)("酵素:リアーゼ[II](タンパク質化学)"泉井桂編)"廣川書店(印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 小林裕和(分担執筆): "光合成事典(日本光合成研究会編)"学会出版センター. 430 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Uemura, N.Shibata, Anwaruzzaman, M.Fujiwara, T.Higuchi, H.Kobayashi, Y.Kai, A.Yokota: "A role of structural intersubunit microheterogeneity in regulation of activity in hysteresis of ribulose 1,5-bisphosphate carboxylase/oxygenase"J.Biochem.. 128. 591-599 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Isono, K.Satoh, H.Kobayashi: "Molecular cloning of a cDNA encoding a novel Ca^<2+>-dependent nuclease of Arabidopsis that is similar to staphylococcal nuclease"Bioch.Biophys.Acta. 1491. 267-272 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Yoshimoto, M.Sakaiya, K.Isono, H.Kobayashi: "Comparison of strength of endogenous and exogenous gene promoters in Arabidopsis chloroplasts"Plant Biotechnol.. 18. 135-142 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Kobayashi, H.Kato, M.Shimizu: "Symbiotic aspects of participation of multiple a factors in functional chloroplasts"Symbiosis and Eukaryotic Organells (M.Sugiura, J.Obokata, eds.), Thuringen University Library, Thuringen. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 加藤秀起, 小林裕和: "σ因子の多様性からみた葉緑体の機能発現"植物細胞工学シリーズ. 17. 114-122 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kobayashi, H., Kato, H.: "Symbiotic aspects of participation of multiplied σ factors in functional chloroplasts. "Symbiosis and Eukaryotic Organells" (Sugiura, M., Obokata, J., eds.)"Logos Verlag Berlin, Berlin(印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yashimoto, K., Sakaiya, M., Isono, K., Kobayashi, H.: "Comparison of strength of endogenous and exogenous gene promoters in Arabidopsis chloroplasts"Plant Biotechnol.. 18. 135-142 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 杉山達夫, 仲本準, 小林裕和: "光合成炭酸固定実験法:細胞から分子まで「生物化学実験法シリーズ」"学会出版センター(印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 小林裕和,小林京子: "新しい高等植物耐塩機構:劣勢遺伝子支配"バイオサイエンスとインダストリー. 58. 489-490 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 杉山達夫,仲本準,小林裕和: "光合成炭酸固定実験法:細胞から分子まで.「生物化学実験法シリーズ」,"学会出版センター(印刷中). (2001)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2000-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi