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Molecular mechanism for the inhibition of photosynthesis by UV and excess photon energy and its proteshon

Research Project

Project/Area Number 12440230
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionFukuyama University

Principal Investigator

ASADA Kozi  Dept. Biotechnology, Fukuyama Univ, professor, 工学部, 教授 (50027182)

Co-Investigator(Kenkyū-buntansha) OGAWA Kenichi  Inst, Biology, Okayama Aes, Fellous, 所長
HARAGUCHI Hiroyuki  Dept, Biotechnology, Fukuyama Univ., Associate Professor, 工学部, 助教授 (10198905)
J. Malcolm Shick  Dept, Biol・ Sci・ Univ of Maine,U・ S・ A, Professor
EVA Hideg  Hungary,Biol, Res・ center,Inst・ Plant Biol, Senior Research Fellow
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2001: ¥3,200,000 (Direct Cost: ¥3,200,000)
Keywordsultra-violet light / ercess photon energg / singlet oxggen / watev-water cycle / 3eta-crystallin / photoinhibition / blue-grean flworescauce / ascorbate peroxidase / ゼータクリスタリン / 青緑蛍光 / アスコルビン酸ペルオキシダーゼ / 紫外光エネルギー / 過剩光エネルギー / Water-Waterサイクル / ゼーター・クリスタリン様タンパク質
Research Abstract

The sunlight is essential for plant growth, but its UV components inhibit photosynthesis. Even the photosynthetically-essential visible components also produces reactive species of oxygen (ROS) and causes the photoinhibition, when leaves are exposed to sunlight intensities in excess of that required for CO_2-fixation. In the present research, the molecular mechanisms of photoinhibition and its protection have been studied on the following topics. 1) Ascorbate peroxidase is the primary target molecules of ROS to induce photoinhibition, 2) Enzymatic properties and physiological functions of zeta-crystallin protein of plants, 3) Monodehydroascorbate reductase is able to catalyze also the reduction of phenoxyl radicals, 4) Induced biosynthesis of blue-green fluorescence components by UV irradiation and its cellular localization, 5) Characteristic properties of the cyclic electron transport around PSI, as revealed by redox change of P700, 6) Detection of singlet oxygen photoproduced in leaf cells, 7) Physiological functions of the water-water cycle in chloroplasts and Zooxanthelle.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Asada, K.: "The water-water cycle as alternative photon and election sinks"Phil, Trans, Roy, Soc, London, β. 355. 1419-1431 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mano, J., Babiychnk, E.et al.: "Diamide oxidoreductase activity in Arabidopsis thaliana Pl Zeta-crystallin"Eur.J.Blochem.. 267. 3661-3671 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mano, J., Yoon, H-J et al.: "Crgstallization and preliminary X-ray crystallographic analysis of NADPl-liquinone oxidoredatasc"Biochim.Biophys.Acta. 279. 949-954 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mano, J., Ohno, C.et al.: "Choloroplastic ascorhate peroxides is the primary target of methylviologen-lnduced photoox dative stress"Biochim.Biophys.Acta. 1504. 275-287 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyake, C., Asada, K.: "The water-water cycle in algae"Photosynthesis and Respiration in Algue. (印刷中). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 浅田浩二: "植物の葉はどうして太陽光の下で緑を保っているのか?"マテリアル学会誌. 13. 114-122 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Asada, K.: "The water-water cycle as alternative photon and electron sinks"Phil. Trans. Roy. Soc. London B. 355. 1419-1431 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mano, J. Babiychuk, E. Belles-Boix, E. Hiratake, J. Kimura, A. Inze, D. Kushnir, S. and Asada, K: "A novel NADPH : diamide oxidoreductase activity in arabidopsis thaliana P1-crystallin"Eur. J. Biochem.. 267. 3661-3671 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mano, J. Yoon, H-J. Asada, K. Babiychuk, E. Inze, D. and Mikami, B: "Crystallization and preliminary X-ray crystallographic analysis of NADPH : quinone oxidoreducatase, a plant-crystallim."Biochim. Biophys. Acta. 1480. 374-376 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakihama, Y. Mano, J. Sano, S. Asada, K. and Yamasaki, H.: "Reduction of phenoxyl radicals mediated by monodehydroascorbate reductase"Biochem. Biophys. Res. Commun. 279. 949-954 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mano, J. Ohno, C. Domae, Y. and Asada, K: "Chloroplastic ascorbate peroxidase is the primary target of methylviologen-induced photooxidative stress in spinach leaves : its relevance to monodehydro-ascorbate detected with in vivo ESR"Biochim. Biophys. Acta. 1504. 275-287 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mano, J. Belles-Boix, E. Babiychuk, E. Asada, K. Van Montagu, M. Inze, D. Kushnir, S. and Slooten, L: "Overexpressed P1-crystallin, a putative antioxidant protein, constitutes an oxygen-dependent alternative electron sink in leaves"Plant physiol. (submitted). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hideg, E. Jahasz, M. Bornman, J. and Asada, K: "The distribution and possible orgin of blue-green fluorescence in control and stressed barley leaves"Photochem. Photobiol. (submitted). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Miyake, C. and Asada, K: "The water-water cycle in algae"In larkum, A. W. D. (ed) Photosynthesis and Respiration in Algae, Kluwer Academic Publ., Dordrecht. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Asada, K.: "The water-water cycle as altemative photou and election sinks"Phil. Trans. Roy. Soc. London. B. 355. 1419-1431 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mano, J., Babiychuk, E.et al.: "A novel NADVH diamide oxidoreductase activity in Arabidopsis thaliana Pl zeta-crystallin"Eur. J. Biochem.. 267. 3661-3671 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mano, J., Yoon, H.J et al.: "Crystallization and preliminary X-ray crystallographic analysis of NADPH quinone oxidoredatasc"Biochim. Biophys. Acta. 279. 949-954 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mano, J., Ohno, C.et al.: "Chloroplastic ascorhate ptroxidase is the primary farget of methylviolon-induced plsotoox datire stress"Biochim. Biophys. Acta. 1504. 275-287 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Miyake, C., Asada, K.: "The water-water cycle in algae"Photosynthesis and Respiration in Algue. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 浅田 浩二: "植物の葉はどうして太陽光の下で緑を保っているのか?"マテリアル学会誌. 13. 114-122 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kozi Asada: "The water-water cycle as alternative photon and electron sinks"Phil.Trans.Roy.Soc.(London) Biol.Sect.. 355. 1419-1431 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Jun'ichi Mano: "A novel NADPH : diamide oxidoreductase activity in Arabidopsis thaliana p1 zeta-crystallin"Eur.J.Biochem.. 267. 3661-3671 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Jun'ichi Mano: "Crystallization and preliminary X-ray crystallographic analysis of NADPH : azodicarboxyl/quinone oxidoreductase"Biochim.Biophys.Acta. 1480. 374-376 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tomonori Kawano: "Aromatic monoamina-induced immediate oxidative burst leading to an increase in cytosolic Ca^<2+> concentration in tobacco suspension culture."Plant cell physiol.. 41. 1251-1258 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tomonori Kawano: "Phenyethylamine-induced generation of reactive oxygen spectes and ascoroate free radicals in Tobacco suspension culture"Plant Cell Physiol.. 41. 1259-1266 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yasuko Sakihamo: "Reduction of phenoxy radicals mediated by monodehydroascorbate reductase"Biochem.Biophys.Res.Commun.. 279. 949-954 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 浅田浩二: "生物の光障害とその防御機構(市橋正光,佐々木政子 編)"共立出版. 15 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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