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Mechanisms of parallel evolution in plant stems and leaves

Research Project

Project/Area Number 12440242
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 系統・分類
Research InstitutionOkazaki National Research Institutes

Principal Investigator

HASEBE Misuyasu  Okazaki National Research Institutes National Institutes for Basic Biology, Professor, 基礎生物学研究所, 教授 (40237996)

Co-Investigator(Kenkyū-buntansha) FUJITA Tomomichi  Okazaki National Research Institutes National Institutes for Basic Biology,Research Associate, 基礎生物学研究所, 助手 (50322631)
TSUKAYA Hirokazu  Okazaki National Research Institutes,Center for Integative Bioscience,Associate Professor, 統合バイオサイエンスセンター, 助教授 (90260512)
MURATA Takashi  Okazaki National Research Institutes National Institutes for Basic Biology,Associate Professor, 基礎生物学研究所, 助教授 (00242024)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥15,300,000 (Direct Cost: ¥15,300,000)
Fiscal Year 2001: ¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 2000: ¥7,300,000 (Direct Cost: ¥7,300,000)
KeywordsKNOX / Physcomitrella patens / sporophyte / gametophyt / generation / auxin / polar transport / shoot / 平行進化 / ホメオボックス / HD-Zip
Research Abstract

1. Class 1 KNOX gene of Physcomitrella patens (PpKNOX 1) was cloned and each GUS and GFP reporter gene was inserted into either 3 and 5 terminal of PpKNOX genomic region using gene targeting technique. The fusion protein was expressed only in the sporophyte generation but not in the gametophyte generation in which stems and leaves develop. This result indicates that molecular mechanisms of stems and leaves between angiosperms and Physcomitrella are different. The ectopic expression of PpKNOX1 gene in Arabidopsis caused the serrated leaves similar to the transformants expressing Arabidopsis class 1 KNOX gene, suggesting that class 1 KNOX function in angiosperms had already established in the common ancestor of angiosperms and Physcomitrella that did not yet have leaves and stems in the sporophyte generation. 2. Polar transport of auxin between gametophores and sporophytes of P. patens, Polytrichum commune, and Bartramia pomiformis was compared. We measured polar auxin transport by a feeding experiment of radiolabelied auxin in either end of the cutting segment of the shoot of mosses, and found that there was little difference of basipetal auxin transport from acropetal transport in gametophores, but obvious polar transport from apical to basal transport was observed in sporophytes. This result also support the hypothesis that leaves and stems of the gametophyte generation in mosses are differently regulated from those of the sporophyte generation in angiosperms.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Shindo, S., Sakakibara, K., Sano, R., Ueda, K., Hasebe, M.: "Chaiacterization of a FLORICAULA/LEAFY homologue of Gnetum parvifolium, and its implications for the evolution of reproductive orgaus in seed plants"Int. J. Plant Sci. 162. 1199-1209 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Himi, S., Sano, R., Nishiyama, T., Tanahashi, T., Kato, M., Ueda, K., Hasebe, M.: "Evolution of MADS-box gene induced by FLO/LFY genes"J. Mol. Evol.. 53. 387-393 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiwatashi, Y., Nishiyama, T., Fujita, T., Hasebe, M.: "Establishment of gene-trap and enhancer-trap systems in the moss Physcomitrella parens"Plant J.. 28. 1-14 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakakibara, K., Nishiyama, T., Kato, M., Hasebe, M.: "Isolation of Homeodomain-Leucine Zipper Genes from the Moss Physcomirrella patens and the Evolution of Homeodomain-Leucine Zipper Genes in Land Plants"Mol. Biol. Evol.. 18. 491-502 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shindo, S., Sakakibara, K., Sano, R., Ueda, K. and Hasebe, M: "Characterizatin of a FLORICAULA/LEAFY homologue of Gnetum parvifoliura, and its implications for the evolution of reproductive organs in seed plants."Int. J. Plant Sci. 162. 1199-1209 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Himi, S., Sano, R., Nishiyama, T., Tanahashi, T. Kato, M., Ueda, K., and Hasebe, M: "Evolution of MADS-box gene induced by FLO/LFY genes."J. Mol. Evol. 53. 387-393 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiwatashi, Y., Nishiyama, T., Fujita, T. and Hasebe, M: "Establishment of gene-trap and enhancer-trap systems in the moss Physcomitrella patens."Plant J. 28;. 1-14 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakakibara, K., Nishiyama, T., Kato, M., and Hasebe, M: "Isolation of Homeodomain-Leucine Zipper Genes from the Moss Physcomitrella patens and the Evolution of Homeodomain-Leucine Zipper Genes in Land Plants."Mol. Biol. Evol. 18:. 491-502 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shindo, S., Sakakibara, K., Sano, R., Ueda.K., Hasebe.M.: "Characterizatin of a FLORICAULA/LEAFY homologue of Gnetum parvifolium and its implications for the evolution of reproductive organs in seed plants"Int. J. Plant Sci.. 162. 1199-1209 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Himi, S., Sano, R., Nishiyama, T., Takahashi, T., Kato, M., Ueda, K., Hasebe, M.: "Evolution of MADS-box gene induced by FLO/LFY genes"J. Mol. Evol.. 53. 387-393 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hiwatashi, Y., Nishiyama, T., Fujita, T., Hasebe, M.: "Establishment of gene-trap and enhancer-trap systems in the moss Physcomitrella patens"Plant J.. 28. 1-14 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sakakibara, K., Nishiyama, T., Kato, M., Hasebe, M.: "Isolation of Homeodomain-Leucine Zipper Genes from the Moss Physcomitrella patens and the Evolution of Homeodomain-Leucine Zipper Genes in Land Plants"Mol. Biol. Evol.. 18. 491-502 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Himi,S.,Sano,R.,Nishiyama,T.,Tanahashi,T.,Kato,M.,Ueda,K.,and Hasebe,M.: "Evolution of MADS-box gene induced by FLO/LFY genes."J.Mol.Evol.. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sakakibara,K.,Nishiyama,T.,Kato,M.,and Hasebe,M.: "Isolation of Homeodomain-Leucine Zipper Genes from the Moss Physcomitrella patens and the Evolution of Homeodomain-Leucine Zipper Genes in Land Plants."Mol.Biol.Evol.. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sano,R.,Ito,M.,Kurita,S.and Hasebe,M.: "Deparia formosana (Rosenst.) as the new Diplazium formosanum."Acta Phototaxa Geobot.. 51. 17-20 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sano,R.,Takamiya,M.,Kurita,S.,Ito,M.and Hasebe,M.: "Diplazium subsinuatum and Di.tomitaroanum should be moved to Deparia according to molecular, morphological, and cytological characters."J.Plant Res.. 113. 157-163 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sano,R.,Takamiya,M.,Ito,M.,Kurita,S.and Hesebe,M: "Phylogeny of lady fern group, tribe Physematieae (Dryopteridaceae) based on chloroplast rbcL gene sequences."Molec.Phyl.Evol.. 15. 403-413 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nishiyama,T.,Hiwatashi,Y.,Sakakibara,K.,Kato,M.and Hasebe,M.: "Tagged mutagenesis and gene-trap in the moss, Physcomitrella patens by shuttle mutagenesis."DNA Res.. 7. 1-9 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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