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Development of Dynamic Sensing System to Detect Environmental Stresses Induced in Living Cell. - Application to Diagnosis of Living Cell

Research Project

Project/Area Number 12450093
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Dynamics/Control
Research InstitutionYamagata University

Principal Investigator

KOSAWADA Tadashi  Yamagata University, Mechanical Systems Engineering, Associate Professor, 工学部, 助教授 (10143083)

Co-Investigator(Kenkyū-buntansha) IIJIMA Yoshiaki  Yamagata University, School of Medicine. Research Associate, 医学部, 助手 (90241710)
KUBOTA Yohko  Yamagata University, School of Medicine, Associate Professor, 医学部, 助教授 (60125763)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 2002: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2001: ¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 2000: ¥5,600,000 (Direct Cost: ¥5,600,000)
KeywordsLiving cell / Environmental stress / Dynamic property / Micro sensor / Vibration / Diagnosis / Environmental hormone / Cultured cartilage / ストレス / 軟骨組織 / 世代間
Research Abstract

In this study, a novel method to detect the environmental stresses induced in cell-like minute object is presented by using dynamic response of piezoelectric vibrator. Experimental studies have shown its capability to detect changes of the environmental stresses induced in egg cells of killifish as well as hardening process of tissue-engineered 3D cultured cartilage. The results can be summarized as follows.
(1) A novel method of sensing to detect the softness of sponge-like soft and cell-like minute object is specifically presented by using dynamic response of piezoelectric vibrator. Experimental studies conducted on silicone rubber blocks, whose Shore hardness, Hs are known as 5-80, have shown the sensor's capability to classify the softness of sponge-like soft object.
(2) With the aid of this sensing system, it is found that the living egg cells of killifish reveal their specific responses to the environments such as changes of temperature, concentration, pH, environmental hormone, etc. of surrounding solution or sucking pressure applied to the outside surface of the cell. Thus, the present sensor system has enough capability to monitor the environmental stresses induced in living cells.
(3) The hardening process of tissue-engineered three dimensionally (3D) cultured cartilage has been studied with respect to culture periods. Dynamic properties as well as biochemical composition were examined. It is found that the present study has a possibility to provide a useful information to decide whether the cultured cartilage has enough hardness for transplantation treatment of articular cartilage damage.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] 小沢田, 佐藤, 山下: "細胞の力学特性検知システムの開発"日本機械学会Dynamics and Design Conf.2000 CD-ROM論文集. No.726. 6 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Kosawada, H.Satoh, H.Nishida: "Development of a Softness Sensing System by Using Dynamic Response of Piezoelectric Vibrator and Its Application to Detect Mechanical Properties of Living Cells"JSME International Journal, Series C. Vol.43, No.4. 772-779 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 小沢田, 佐藤, 山下: "卵細胞の環境ストレス検知システムの開発"日本機械学会バイオエンジニアリング講演会論文集. No.00-35. 286-287 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Kosawada, H.Satoh, T.Yamashita: "Piezo Micro Probe Sensor System to Detect Environmental Stresses Induced in Living Cells"Proceedings of Joint Symposium on Bio-Sensing and Bio-Imaging, Yamagata. 24 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 小沢田, 山村, 今野, 金沢: "3次元微小生体組織の力学特性検知システム"日本機械学会2002年次大会講演論文集. Vol.1, No.02-1. 109-110 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 小沢田, 今野, 山村, 金沢: "ピエゾマイクロ振動センサーを用いる3次元微小細胞組織の力学特性検知システム"日本機械学会東北支部第38期総会・講演論文集. No.031-1. 180-181 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Kosawada, H.Satoh, T.Yamashita: "Development of Sensing System for Dynamic Characteristics of Living Cells"Proceedings of the JSME Dynamics & Design Conference 2000. No.726. 6 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Kosawada, H.Satoh, H.Nishida: "Development of a Softness Sensing System by Using Dynamic Response of Piezoelectric Vibrator and Its Application to Detect Mechanical Properties of Living Cells"JSME International Journal, Series C. Vol.43,No.4. 772-779 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Kosawada, H.Satoh, T.Yamashita: "Development of Sensing System for Environmentally Induced Mechanical Stresses of Egg Cells"Proceedings of the JSME 13-th Bioengineering Conference. No.OO-35. 286-287 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Kosawada, H.Satoh, T.Yamashita: "Piezo Micro Probe Sensor System to Detect Environmental Stresses Induced in Living Cells"Proceedings of Joint Symposium on Bio-Sensing and Bio-Imaging, Yamagata. 24 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Kosawada, S.Yamamura, K.Konno, T.Kanazawa: "Sensing System for Dynamic Properties of the Three Dimensional Minute Living Body"Proceedings of the JSME Annual Meeting Conference 2002. Vol.1, No.02-1. 109-110 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Kosawada, K.Konno, S.Yamamura, T.Kanazawa: "Sensing System to Detect Mechanical Properties of Three Dimensional Minute Living Cells"Proceedings of the JSME 37-th Tohoku Annual Meeting Conference. No.031-1. 180-181 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 小沢田, 山村, 今野, 金沢: "3次元微小生体組織の力学特性検地システム"日本機械学会2002年次大会講演論文集. Vol.1,No.02-1. 109-110 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小沢田, 遠藤, 市川: "2次元微小生体軟組織片の動的力学物性値評価法の開発"日本機械学会2002年次大会講演論文集. Vol.1,No.02-1. 107-108 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小沢田, 今野, 山村, 金沢: "ピエゾマイクロ振動センサーを用いる3次元微小細胞組織の力学特性検知システム"日本機械学会東北支部第38期総会・講演論文集. No.031-1. 180-181 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小沢田, 市川, 遠藤: "はりの強制振動を利用した2次元微小生体軟組織片の動的力学物性値評価法"日本機械学会東北支部第38期総会・講演論文集. No.031-1. 178-179 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小沢田, 松川: "血管内皮細胞内チャネルの形成メカニクス"日本機械学会東北支部第38期総会・講演論文集. No.031-1. 170-171 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小沢田, 井上: "微小毛細血管内皮細胞内小胞の形成と変形のメカニクス"日本機械学会東北支部第38期総会・講演論文集. No.031-1. 172-173 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Kosawada, H.Satoh, T.Yamashita: "Piezo Micro Probe Sensor System to Detect Environmental Stresses Induced in Living Cell"Proc.Joint Symposium on Bio-Sensing and Bio-Imaging. 24-24 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 小野寺 弘貴, 小沢田 正: "細胞内小胞膜シェルの大変形解析"Dynamics & Design Conference 2001. CD-ROMアブスト集. 67-67 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Kosawada, K.Sanada, T.Takano: "Large Deformation Mechanics of Plasma Membrane Chained Vesicles in Cells"JSME International Journal. 44-4. 928-936 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 小沢田 正, 黒田 賢一郎, 遠藤 正史: "バイオプシー微小生体軟組織片の動的力学物性値評価法の開発"ジョイント・シンポジウム2001 スポーツ工学シンポジウム:ヒューマン・ダイナミクス論文集. No.01-22. 165-169 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 小沢田 正, 佐藤英明, 山下知成: "卵細胞の環境ストレス検知システムの開発"第13回バイオエンジニアリング講演会論文集. No.00-35. 286-287 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 小沢田 正, 真田 宏一, 高橋 友和, 高野 哲夫: "細胞内膜動輸送機構のメカニクスとシミュレーション"第13回バイオエンジニアリング講演会論文集. No.00-35. 178-179 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tadashi,Kosawada,Hideak,Sata Hajime Nishida: "Development of Softness Sensing System by Using Dynamic Response of Piezoelectric Vibrator and Its Application to Detect Mechanical Properties of Living Cells "JSME International Journal. Series C Vol.43-4. 772-779 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 小沢田正,佐藤英明,山下智成: "細胞の力学特性検知システムの開発"日本機械学会Dynamics & Design Conference 2000 論文集. アブスト集+CD-ROM. 386-386 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 小沢田正,高野哲夫,高橋友和,真田宏一: "細胞内膜動輸送機構の力学的解析"日本機械学会Dynamics&Design Conference 2000論文集. アブスト集+CD-ROM. 387-387 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 小沢田正,佐藤英明,山下智成: "卵細胞の環境ストレス検知システムの開発"日本機械学会第13回バイオエンジニアリング講演会論文集. No.00-35. 286-287 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 小沢田正,真田宏一,高橋友和,高野哲夫: "細胞内膜動輸送機構のメカニクスとシミュレーション"日本機械学会第13回バイオエンジニアリング講演会論文集. No.00-35. 178-179 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 小沢田正,山下智成,伊藤幸人: "細胞の力学特性検知システムの開発"日本機械学会東北学生会第30回卒業研究発表講演会論文集. 85-86 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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