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Development of Artificial Enzymes by Surface Molecular Imprinting

Research Project

Project/Area Number 12450335
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物・生体工学
Research InstitutionSojo University (2001)
Kyushu University (2000)

Principal Investigator

FURUSAKI Shintaro  Sojo university, Faculty of Engineering, Professor, 工学部, 教授 (40011209)

Co-Investigator(Kenkyū-buntansha) UEZU Kazuya  The University of Kitakyushu, Faculty of Environmental Engineering, Assistant Professor, 国際環境工学部, 助教授 (40253497)
GOTO Masahiro  Kyushu University, Dept. of Chemical Systems & Engineering, Professor, 大学院・工学研究院, 教授 (10211921)
YAMAMOTO Shinjiro  Sojo university, Faculty of Engineering, Professor, 工学部, 講師 (40262307)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 2001: ¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 2000: ¥7,900,000 (Direct Cost: ¥7,900,000)
KeywordsEnzyme Reaction / Molecular Imprinting / Surfactant / Chirality Recognition / Imprinted Polymer / Optical Resolution / 界面鋳型重合 / 分子インプリント / 生体触媒 / エマルション / 鋳型重合 / 分子インプリント法 / 分子認識 / 不斉反応
Research Abstract

A molecular imprinting technique is one of the promising approaches to design "tailor-made" catalytic sites. However, it still has two fundamental drawbacks : difficulties to introduce water-soluble substrates as an imprinting molecule in the catalytic sites, and slow catalytic kinetics arising from the inner diffusion of substrates toward the catalytic sites formed deeply in the polymer matrix. To conquer these problems, we proposed a novel molecular imprinting technique for constructing recognition sites on the polymer surface called "surface molecular imprinting technique". An imprinted polymer was prepared by polymerizing water-in-oil (W/0) emulsions containing a functional host molecule, an imprint molecule and a cross-linking agent.
In this study, we designed a novel functional host molecule possessing a high inter facial activity, considering that the host molecule should be located at water-oil interface to yield a high specific recognition. Furthermore we introduced an imidazole molecule as a functional group to catalyze the hydrolysis reaction of substrates. Using the newly synthesized functional host molecule, an enzyme-mimic polymer was prepared by imprinting a substrate analogue through the complex formation between a cobalt ion and the imidazole moiety. The catalytic properties of artificial biocatalysts were investigated on the hydrolysis reaction of an amino acid ester by comparing to control experiments. The enzyme-mimic biocatalyst showed a high activity and maintained high stability for a long time.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] M.Yoshida, Y.Hatate, K.Uezu, M.Goto, S.Furusaki: "Metal-Imprinted Microsphere Prepared by Surface Template Polymerization and Its Application to Chromatography"J. Poly. Sci : Part A : Polymer Chemistry. Vol.38. 689-696 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Yoshida, K.Uezu, M.Goto, S.Furusaki: "Surface Imprinted Polymers Recognizing Amino Acid Chirality"J. Appl. Poly. Sci.. 78.No.4. 695-703 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Yoshida, Y.Hatate, K.Uezu, M.Goto, S.Furusaki: "Chiral-recognition polymer prepared by surface molecular imprinting technique"Colloids and Surfaces A : Phy. Eng. Asp.. 169. 258-269 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Araki, M.Yoshida, K.Uezu, M.Goto, S.Furusaki: "Lanthanide-Imprinted Resins Prepared by Surface Template"J. Chem. Eng. Japan,. Vol.33 No.4. 665-668 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shinya.OKAZAKI, Masahiro GOTO, Shintaro FURUSAKI: "Preparation and Catalytic Performance of Surfactant-Manganese Peroxidase-MnII Ternary Comple in Organic Media"Enzyme and Microbial Technology. 28. 329-332 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masahiro GOTO, Shintaro FURUSAKI: "Catalytic Properties of Lignin Peroxidase A LiP-P3 Hosted in Reversed Micelles"Biochemical Engineering J.. Vol.8, No.2. 129-134 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Yoshida, Y.Hatate, K.Uezu, M.Goto, S.Furusaki: "Metal-Imprinted Microsphere Prepared by Surface Template Polymerization and Its Application to Chromatography"J. Poly. Sci. : Part A : Polymer Chemistry. Vol.38. 689-696 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Yoshida, K.Uezu, M.Goto, S.Furusaki: "Surface Imprinted Polymers Recognizing Amino Acid C hiralityJ"J. Appl. Poly. Sci.. 78, No.4. 695-703 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Yoshida, Y.Hatate, K.Uezu, M.Goto, S.Furusaki: "Chiral-recognition polymer prepared by surface molecular imprinting techniques"Colloids and Surfaces A : Phy Eng. Asp.. 169. 259-269 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Araki, M.Yoshida, K.Uezu, M.Goto, S.Furusaki: "Lanthanide-Imprinted Resins Prepared by Surface Template Polyierization"J. Chem. Eng. Japan. Vol.33, No.4. 665-668 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Okazaki, M.Goto, S.Furusaki, H.Wariishi, H.Tanaka: "Preparation and Catalytic Performance of Surfactant-Manganese Peroxidase-Mnil Ternary Compie in 0rganic Media"Enzyme and Microbial Technology. 28. 329-332 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] J.Michizoe, S.Okazaki, M.Goto, S.Furusaki: "Catalytic Properties of Lignin Peroxidase ALiP-P3 Hosted in Reversed Micelles"Biochemical Engineering. Vol.8, No.2. 129-134 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] J.Michizoe, M.Goto, S.Furusaki: "Catalytic Activity of Laccase Hosted in Reversed M icelles"J. Bioscience and Bioeng.. Vol.92, No.1. 67-71 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsunemori H., Araki K., Uezu K., Goto Masahiro, Furusaki Shintaro: "Surface Imprinting Polymers for the Separation of Nucleotides"Bioseparation. Vol.10. (in press) (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 上江洲一也, 後藤雅宏, 古崎新太郎: "界面分子インプリントポリマーのマトリクスに影響を与える因子の解明とその制御"化学工学論文集. 第27巻第6号. 753-755 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shinya.OKAZAKI, Masahiro GOTO, Shintaro FURUSAKI: "Preparation and Catalytic Performance of Surfactant-Manganese Peroxidase-MnII Ternary Comple in Organic Media"Enzyme and Microbial Technology. 28. 329-332 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Masahiro GOTO, Shintaro FURUSAKI: "Chataritic performance of Lignine Peroxidase Alip-P3 in reversed Micelles"Biochemical Engineering J.. Vol.8, No.2. 129-134 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Masahiro GOTO, Shintaro FURUSAKI: "Catalytic Activity of Laccase Hosted in Reversed Micelles"J. Bioscience and Bioeng.. Vol.92, No.1. 67-71 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Goto et al.,: "Metal-Imprinted Microsphere Prepared by Surface Template Polymerization and Its Application to Chromatography"J.Poly.Sci : Part A : Polymer Chemistry. 38. 389-396 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Goto et al.,: "Surface Imprinted Polymers Recognizing Amino Acid Chirality"J.Appl.Poly.Sci.,. 78・4. 695-703 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Goto et al.,: "Lanthanide-Imprinted Resins Prepared by Surface Template Polymerization"J,Chem.Eng.Japan. 33・4. 665-668 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Goto et al.,: "Chiral-recognition polymet prepared by surface molecular imprinting technique"Colloids and Surfaces A : Phy.Eng.Asp.,. 169. 259-269 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 後藤雅宏,古崎新太郎: "界面分子インプリント法による希土類認識材料の調製"化学工学シンポジウムシリーズ. 74. 96-102 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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