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Elucidation of Reaction Mechanism of Abnormal Heme Metabolism by Synthetic Methods

Research Project

Project/Area Number 12450368
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Synthetic chemistry
Research InstitutionFukui National College of Technology

Principal Investigator

OGOSHI Hisanobu  Fukui National College of Technology, President, 校長 (90026188)

Co-Investigator(Kenkyū-buntansha) HAYASHI Takashi  Depertment of Chemistry and Biolchemistry, Graduate School of Engineering, Kyushu University, Associate Professor, 大学院・工学研究科, 助教授 (20222226)
TSUDA Yoshihiro  Fukui National College of Technology, Depertment of Chemistry and Biology Engineering, Associate Professor, 物質工学科, 助教授 (60216463)
MATSUI Syuichi  Fukui National College of Technology, Depertment of Chemistry and Biology Engineering, Professor, 物質工学科, 教授 (60042975)
AOYAGI Katsuhiro  Fukushima National College of Technology Department of Chemistry and Biochemistry, Professor, 物質工学科, 教授 (40150940)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2001: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2000: ¥11,000,000 (Direct Cost: ¥11,000,000)
KeywordsProtein Reconstitution / Heme Protein / N-Alkylsubstituted Porphyrin / Myoglobin / Heme Abnormal Metabolism / Normal / Reverse / Heme Pocket / NMR
Research Abstract

Cobalt(II)complexes of N_A-ethyl and N_B-ethylmesoporphyrin dimethylester(the isomers with ethyl group on the A ring and B rings) have been prepared to elucidate the reaction mechanism of abnormal heme metabolism. The reconstituted myoglobins with the cobalt(II) complexes of N-ethylmesoporphyrin have been characterized by 1^H-NMR measurements.
The reconstitution of apomyoglobin with 1.6 equivalent of Co(II)complexes of N_A-and N_B-ethylmesoporphyrin affordd stable myoglobins ((N_A-C_2H_5Meso)Co(II) and (N_B-C_2H_5Meso)Co(II)-Mb. The 1^H-NMR spectrum showed three signals at -34.75 ppm, -36.63 ppm and -48.61 ppm assigned to the methyl group of N-ethyl group, respectively.
The chemical shifts of these signals were agreement with those of myoglobins reconstituted with (N_A-C_2H_5 Meso Co(II) and (N_B-C_2H_5Meso)Co(II), individually. The N-alkylmesoporphyrin coordinates with myoglobin in normal or reverse orientation because the heme pocket of yoglobin recognizes the sequence of side chains o … More f N-alkylmesoporphyrin. The signal at -34.75, -36.63 and -48.61ppm is assigned to the NA-CzHs of reverse orientation, N_A-C_2H_5 of normal orientation and N_B-C_2H_5 of reverse orientation, respectively. It seems that the steric repulsion of Na-ethyl group with isopropyl group of Val 68 in myoglobin is larger than that of N_A-ethyl group is responsible for the relative abundance of N_A-and N_B-ehyl group of reverse orientation(1 : 0.4). In addition, it seems that the relative abundance of N_A-ethyl group of reverse rientation and N_B-ethyl group of normal orientation (1 : 2) is dependent on the assumption that the N-ethylmesoporphyrin of normal orientation is referentially reconstituted with myoglobin because the heme pocket of myoglobin recognize the sequence of side chains of N-alkylmesoporphyrin.
It can be seen from these results that the apomyoglobin recognizes a pair of enantiomers of N-alkylmesoporphyrin by the asymmetric heme pocket, and that the steric repulsion of N-ethyl group with Val 68 play an important role in the asymmetric recognition. It seems reasonable to assume that the relative abundance of the regioisomers of N-alkylmesoporphyrin reconstituted with myoglobin is tendency to migrate to pyrolle nitrogen atom of alkyl group. Less

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] Katsuhiro Aoyagi: "Studies on Pseudomonas aeruginosa cdl Nitrite Reductase : The Association and Dissociation Reactions of the dl-Heme"Israel Journal of Chemistry. 40. 27-33 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takashi Hayashi: "Introduction of a Specific Binding Domain on Mioglobin Surface by New Chemical Modification"J.Inorg.Biochem. 82. 133-139 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takashi Hayashi: "Synthesis and Properties of Alkylperoxocobalt(III) Porphyrin Porphycene"Chem.Lett.. 90-91 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takashi Hayashi: "Electrochemical Reactions Mediated by Vitamin B12 Derivatives in Organic Solvents"Cood.Chem.Rev.. 198. 21-37 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hisanobu Ogoshi: "Interprotein Electron Transfer Reaction Regulated by an Artificial Interface"Angew.Chem.Int.Ed.Engl.. 40. 1098-1101 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takashi Hayashi: "First Catalysis of Cobaltporphycenes. Oxidation of Vinyl Ether via Formation and Dissociation of Cobalt-Carbon Bond"Organometallics. 20. 3074-3078 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takashi Hayashi: "Formation and Cleavage of a Dicobalt Complex Bridged with a Pentamethylene Group"Chem.Lett.. 346-347 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takashi Hayashi: "Chemical Properties of Reconstituted Myoglobins with Functionalized Hemin Derivatives"J.Inorg.Biochem.. 86. 57 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takashi Hayashi: "New Myoglobin Reconstituted with an Iron Porphycene as a Structural Isomer of Heme Moiety"J.Inorg.Biochem.. 86. 200 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takashi Hayashi: "Photoinduced Electron Transfer within an Artificially Created Protein Complex"J.Inorg.Biochem.. 86. 124 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takashi Hayashi: "New Functionalization of Myoglobin by Chemical Modification of Heme Propionates"Acc.Chem.Res.. 35. 35-43 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hisanobu Ogoshi: "Porphyrins and Metalloporphyrins as Receptor Models in Molecular Recognition"Eds. K.Kadish, K.M.Smith, R.Guilard, Academic Press, San Diego, 2000. 62(279-340) (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Ogoshi, T.Mizutani, T.Hayashi, Y, Kuroda: "Porphrins and Metalloporphyrins as Receptor Models in Molecular Recognition"The Porphyrin Handbook. Vol. 6, Chapt 46, 279-340 (Eds. K. Kadish, K. M. Smith, R. Guilard, Academic Press, San Diego. 82.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Takashi, T.Ando, T.Matsuda, H.Yonemura, S.Yamada, Y, Hisaeda: "Introdauction of a Specific Binding Domain on Myoglobin Surface by New Chemical Modification"J. Inorg. Biochem. 82. 133-139

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Takashi, K.Okazaki, H.Shimakoshi, F.Tani, Y.Naruta: "Synthesis and Properties of AIkylperoxocobalt (III) Porohyrin and Porphycene"Chem. Lett. 90-91

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Hisaeda, T.Nishioka, Y.Inoue, K.Asada, T.Hayashi: "Electrochemical Reactions Mediated by Vitamin B12 Derivatives in Organic Solvents"Coord. Chem. Rev. 198. 21-37

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Hitomi, T.Hayashi, K.Wada, T.Mizutani, Y.Hisaeda, H.Ogoshi: "Interprotein Electron Transfer Reaction Regulated by an Artificial Interface"Angew. Chem. Int. Ed. Engl.. 40. 1098-1101

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Hayashi, K.Okazaki, N.Urakawa, H.Shimakoshi Jonathan I, Sessler, Emanuel, Vogel, Y, Hisaeda: "First Catalysis of Cobaltporphycene. Oxidation of Vinyl Ether via Formation and Dissociation of Cobalt-Carbon Bond"Organometallics. 20. 3074-3078

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Shimakoshi, M.Koga, T.Hayashi, Y, Tachi, Y.Naruta, Y, Hisaeda: "Formation and Cleavage of a Dicobalt Complex Bridged with a Pentamethylene Group"Chem. Lett. 346-347

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Hayashi, TAndo, T.Matsuda, H.Sato, T.Matsuo, Y.Hisaeda: "Chemical Properties of Reeonstituted Myoglobins with Functionalyzed Hemin Derivatives"J. Inorg. Biochem. 86. 57

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Dejima, T.Hayashi, T.Matsuo, Y.Hisaeda: "New Myoglobin Reconstituted with an Iron Porphycene as a Structureal Isomer of Heme Moiety"J. Inorg. Biochem. 86. 200

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] TAndo, T.Hayashi, H.Sato, Y.Hisaeda: "Photoinduced Electron Transfer within an Artificially Created Protein-Protein Complex"J. Inorg. Biochem. 86. 124

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] THayashi, Y.Hisaeda: "New Functionalization of Myoglobin by Chemical Modification of Heme-Propionates"Acc. Chem. Res.. 35. 35-43

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Hayashi, T.Aya, M.Nonoguchi, T.Mizutani, Y.Hisaeda, S.Kitagawa, H.Ogoshi: "Chiral Recognition and chiral sensing by Use of Zinc Porphyrin Dimers"Terahedron.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Aoyagi: "Studies on Pseudomonas aeruginosa cdl Nitrite Reductase : The Association and Dissociation Reactions of the d1-Heme"Israel Journal of Chemistry. 40. 27-33

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Aoyagi, T.Haga: "Synthesis and Spectroscopic, meso-Tetrakis (trifluoromethyl) porphyrin."Reserch Reports of Fukushima National College of Technology. 40. 53-57

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsuhiro Aoyagi: "Studies on Pseudomonas aeruginosa cdl Nitrite Reductase : The Association and Dissociation Reactions of the dl-Heme"Israel Journal of Chemistry. 40. 27-33 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takashi Hayashi: "Introduction of a Specific Binding Domain on Mioglobin Surface by New Chemical Modification"J. Inorg. Biochem. 82. 133-139 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takashi Hayashi: "Synthesis and Properties of Alkylperoxocobalt(III) Porphyrin Porphycene"Chem. Lett.. 90-91 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takashi Hayashi: "Electrochemical Reactions Mediated by Vitamin B12 Derivatives in Organic Solvents"Cood. Chem. Rev.. 198. 21-37 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hisanobu Ogoshi: "Interprotein Electron Transfer Reaction Regulated by an Artificial Interface"Angew. Chem. Int. Ed. Engl.. 40. 1098-1101 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takashi Hayashi: "First Catalysis of Cobaltporphycenes. Oxidation of Vinyl Ether via Formation and Dissociation of Cobalt-Carbon Bond"Organometallics. 20. 3074-3078 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takashi Hayashi: "Formation and Cleavage of a Dicobalt Complex Bridged with a Pontamothylono Group"Chem. Lett.. 346-347 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takashi Hayashi: "Chemical Properties of Reconstituted Myoglobins with Functionalized Homin Dorivativos"J. Inorg. Biochem.. 86. 57 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takashi Hayashi: "Now Myoglobin Reconstituted with an Iron Porphycene as a Structural Isomor of Homo Moioty"J. Inorg. Biochem.. 86. 200 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takashi Hayashi: "Photoinduced Electron Transfer within an Artificially Greated Protein Complox"J. Inorg. Biochem.. 86. 124 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takashi Hayashi: "New Functionalization of Myoglobin by Chemical Modification of Heme Propionatos"Acc. Chem. Res.. 35. 35-43 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hisanobu Ogoshi: "Porphyrins and Metalloporphyrins as Receptor Models in Molecular Recognition"Eds. K.Kadish, K.M.Smith, R.Guilard, Academic Press, San Diego,2000. 62 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] H.Ogoshi and T.Mizutani: "Novel Approach to Molecular Recognition Using Porphyrins"Current Opinion in Chemical Biology. 3.No6. 736-739 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Mizutani,N.Sakai,S.Tagi,T.Takagishi and H.Ogoshi: "Allosteric Chirality Amplification in Zinc Bilinone Dimer"J.Am.Chem.Soc.. 122. 748-749 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Hitomi,T.Hayashi,T.Mizutani,Y.Hisaeda and H.Ogoshi: "Interprotein Electron Transfer Reaction by an Artical Interface Artical Interface"Angew.Chem.Int.Ed.Engl..

    • Related Report
      2000 Annual Research Report
  • [Publications] K.Aoyagi and T.Haga: "Synthesis and Spectroscopic, Electrochemical Properties of meso-Tetrakis (trifluoromethyl) porphyrin."Research Reports Fukusima National College of Technology. 40. 53-57 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Ogoshi,T.Mizutani,T.Hayashi and Y.Kuroda: "The Porphrin Hndbook.Vol.6, Chapt 46, Eds.K.Kadish, K.M.Smith,R.Guilard"Academic Press, San Diego. 62 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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