• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Isolation and functional analysis of genes that regulate development and morphogenesis in rice.

Research Project

Project/Area Number 12460002
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

HIRANO Hiro-yuki  Graduate School of Agricultural and Life Sciences, Associate Professor, 農学生命科学研究科, 助教授 (00192716)

Co-Investigator(Kenkyū-buntansha) NAGATO Yasuo  Graduate School of Agric and Life Sciences, Professor, 農学生命科学研究科, 教授 (10143413)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2001: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2000: ¥9,300,000 (Direct Cost: ¥9,300,000)
KeywordsDL gene / YABBY gene / meristem size / development and differentiation / carpel / floral meristem / homeotic mutation / rice (Oryza sativa) / ホメオティク変異 / 花 / トウモロコシ / コムギ / 器官決定
Research Abstract

In this project, we studied on the isolation and analysis of genes that regulate flower and leaf development in rice.
(1) The mutation occurred in the dl locus causes homeotic transformation of carpels into stamens in the flower and loss of midrib in the leaf. We analyzed phenotypes of the dl mutants and spatial expression patterns of the DL gene and revealed that DL regulates carpel specification and midrib formation. We also indicate that DL is antagonistically interacted with class B MADS genes.
(2) We isolated the DL orthologs from maize and wheat and analyzed their spatial expression patterns. The result indicated that the DL functions controlling carpel specification and midrib formation are conserved in these two plants.
(3) DL is a member of the YABBY gene family. We isolated seven YABBY genes from rice and analyzed structural features of YABBY proteins. Spatial expression patterns of each YABBY gene were distinct from those of the Arabidopsis YABBY genes, suggesting that the functions of rice YABBY genes are diversified from those of Arabidopsis.
(4) The rice fon1 mutant have increased number of floral organs. This phenotype seems to be caused by an enlargement of floral meristem. We tried to isolate this gene by positional cloning and found a putative FON1 gene.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Kikuchi, K.: "Molecular analysis of the NAC gene family in rice"Mol. and Gen. Genet.. 262. 1047-1051 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harada, K.: "Isolation and structural analysis of the YABBY gene family in rice"Rice Genet. Newslett. 17. 101-103 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirano, H.-Y.: "Comparison of Waxy gene regulation in the endosperm and pollen in Oryza sativa L"Genes Genetic Syst.. 75. 245-249 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato, Y.: "Mutations that cause amino acid substitutions at the invariant positions in homeodomain of OSH3 KNOX protein suggest artificial selection during rice domestication"Genes Genet. Syst.. 76. 381-392 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kikuchi, K.: "The plant MITE mPing is mobilized in anther culture"Nature. 421. 167-170 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagasawa, N.: "SUPERWOMAN 1 and DROOPING LEAF genes control floral organ identity in rice"Development. 130. 705-718 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kikuchi,K., M.Ueguchi-Tanaka, K.T.Yoshida, Y.Nagato, M.Matsusoka, and H.-Y.Hirano: "Molecular analysis of the NAC gene family in rice"Mol. and Gen. Genet. 262. 1047-1051 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harada,K., Yamaguchi,T., and Hirano H.-Y.: "Isolation and structural analysis of the YABBY gene family in rice"Rice Genet. Newslett. 17. 101-103 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirano, H.-Y. and Y.Sano: "Comparison of Waxy gene regulation in the endosperm and pollen in Oryza sativa L"Genes Genetic Syst.. 75. 245-249 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato,Y., Y.Fukuda, and H.-Y.Hirano: "Mutations that cause amino acid substitutions at the invariant positions in homeodomain of OSH3 KNOX protein suggest artificial selection during rice domestication"Genes Genet. Syst.. 76. 381-392 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kikuchi,K., K.Terauchi, M.Wada, H.-Y.Hirano: "The plant MITE mPing is mobilized in anther culture"Nature. 421. 167-170 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagasawa,N., M.Miyoshi, Y.Sano, H.Satoh, H.-Y.Hirano, H.Sakai H., and Y.Nagato.: "SUPERWOMAN 1 and DROOPING LEAF genes control floral organ identity in rice"Development. 130. 705-718 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kikuchi, K.: "The plant MITE mPing is mobilized in anther culture"Nature. 421. 167-170 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nagasawa, N.: "SUPERWOMAN 1 and DROOPING LEAF genes control floral organ identity in rice"Development. 130. 705-718 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suzuki, Y.: "Isolation and characterization of a rice mutant insensitive to cool temperatures on Wx gene regulation and amylose synthesis"Euphytica. 123. 95-100 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sato, Y. et al.: "Mutations that cause amino acid substitutions at the invariant positions in homeodomain of OSH3 KONX protein suggest artificial selection during rice"Genes Genet. Syst.. 76. 381-392 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sato, Y. et al.: "Auxin response factor family in rice"Genes Genet. Syst.. 76. 373-380 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 佐藤: "イネ品種「ミルキークイーン」の低アミロース性の遺伝子分析"育種学研究 3 13-19. 3. 13-19 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Suzuki, Y.: "Isolation and characterization of a rice mutant insensitive to cool temperatures on Wx gene regulation and amylose synthesis"Euphytica. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Kikuchi,K.: "Molecular analysis of the NAC gene family in rice."Mol.Gen.Genet.. 262. 1047-1051 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hirano,H.-Y.: "Comparison of Waxy gene regulation in the endosperm and pollen in Oryza sativa L."Genes Genet.Syst.. 75. 245-249 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Inukai,T.: "Analysis of intragenic recombination at wx in rice : Correlation between the molecular and genetic maps within the locus."Genome. 43. 589-596 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mikami,I.: "Effects of the two most common Wx alleles on different genetic backgrounds in rice."Plant Breed.. 119. 505-508 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Harada,K.: "Isolation and structural analysis of the YABBY gene family in rice."Rice Genet.Newslett.. 17(in press). (2000)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2000-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi