| Project/Area Number |
12460022
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
植物保護
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
KAWAKITA Kazuhiro Nagoya University, Graduate School of Bioagricultural Sciences Associate Professor, 大学院・生命農学研究科, 助教授 (90186065)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YOSHIOKA Hirofumi Nagoya University, Graduate School of Bioagricultural Sciences Assistant Professor, 大学院・生命農学研究科, 助手 (30240245)
DOKE Noriyuki Nagoya University, Graduate School of Bioagricultural Sciences Professor, 大学院・生命農学研究科, 教授 (80023472)
|
|---|
| Project Period (FY) |
2000 – 2002
|
| Project Status |
Completed (Fiscal Year 2002)
|
| Budget Amount *help |
¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2000: ¥10,000,000 (Direct Cost: ¥10,000,000)
|
|---|
| Keywords | Defence Response / Tabacco / Potato / Elicitor / Phytophthora infestans / Differential Display / Differential Hybridization / Subtraction / 抵抗性 / Differential hybridization / LRRレセプター / サリチル酸 / ロイシンリッチリピート / レセプター |
|---|
| Research Abstract |
The aim of this study is to screen novel resistance-related genes and analyze the function of these genes in plant defense responses against pathogen attack. We employed differential hybridization, subtraction and differential display methods to isolate elicitor-inducible genes of tobacco and potato using the plant-fungal elicitor from Phytophthora infestans system. Sequence analysis of cDNAs identified by differential hybridization in tobacco revealed similarities to genes for SAR8.2, glycine-rich protein, extensin, acyltransferase. Sequence analysis of cDNAs identified by subtraction in potato revealed similarities to genes for pathogenesis-related (PR) genes, glutathione-S-transferase, peroxidase, catalase, 1-aminocylopropane-1-carboxylic acid oxidase, and wound-inducible genes. Sequence analysis of cDNAs identified by differential hybridization in potato revealed similarities to genes for tyramine hydroxycinnamoyl transferase, glucose 6-phosphate dehydrogenase, pyruvate decarboxylse, and defense related-genes. We analyzed the function of two novel genes isolated by differential display in tobacco. One is elicitor-inducible cytochrome P450 gene designated CYP82E1. There is the possibility that CYP82E1 act in biosynthesis of barrier or antifungal compounds to limit invasion of pathogens. The other gene codes for a leucine-rich repeat (LRR) protein closely related to Cf genes in tomato and designated EILP (Elicitor-Inducible LRR Protein) gene. In transgenic tobacco plants overexpressing EILP, expression of defense-related genes against various elicitors increased and resistance against pathogenic bacteria was activated, implying that the product of EILP acts as a receptor-like protein amplifying signals in plants infected with pathogen and elicit defense responses.
|
