| Project/Area Number |
12460023
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物保護
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
ICHINOSE Yuki Okayama University, Faculty of Agriculture, Professor, 農学部, 教授 (50213004)
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| Co-Investigator(Kenkyū-buntansha) |
TOYODA Kazuhiro Okayama University, Graduate School of National Science and Technology, Assistant Professor, 自然科学研究科, 助手 (50294442)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 2002: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2001: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2000: ¥5,700,000 (Direct Cost: ¥5,700,000)
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| Keywords | defense responses / elicitor / transcription factors / Dof proteins / zinc finger |
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| Research Abstract |
In this study, we investigated the structure and function of novel transcription factor E84 (ERDP) that is isolated from elicitor-treated pea epicotyls. E84 is a plant specific transcription factor possessing Dof-type DNA binding domain at the N-terminus. Random site selection assay for E84 revealed that the recombinant E84 protein binds to AAAG-containing DNA fragments. DNA binding assay using a model DNA fragment, BS4/37 (TCATTTAAAGTGTTTT) that could bind to E84, revealed that not only AAAG core sequence but also 3'-TGT sequence were required for high affinity of the binding. E84 protein also bound to the DNA fragments in the promoter of the elicitor-responsive gene, PsCHS1, which contain AAAG sequence. When we carried out transient transfection assay with pea protoplasts and reporter plasmid possessing 4 repeats of "BS4/37"/CaMV 35S minimal promoter plus reporter gene, we observed that the elicitor enhanced reporter gene activity, indicating the possible involvement of AAAG sequence in elicitor-mediated transcriptional activation. We also observed that E84 protein binds to the promoter sequence of E84 gene itself, indicating possible mechanism of auto-regulation. These results show that E84 protein and its binding sequences might contribute to the elicitor-mediated transcriptional regulation. Furthermore, we also found that at least 7 members of the Dof gene family in pea. These observations provide the information to elucidate the mechanism of the transcriptional activation for plant defense-response related genes.
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