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Effects of gene expressions in companion cells on the growth of higher plants

Research Project

Project/Area Number 12460029
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

HAYASHI Hiroaki  Graduate School of Agricultural and Life Sciences, Associate Professor, 大学院・農学生命科学研究科, 助教授 (60180973)

Co-Investigator(Kenkyū-buntansha) FUJIWARA Toru  Graduate School of Agricultural and Life Sciences, Research Assistant, 大学院・農学生命科学研究科, 助手 (80242163)
YAMAYA Tomoyuki  Tohoku University, Graduate School of Agricultural Science, Professor, 大学院・農学研究科, 教授 (30144778)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 2002: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2001: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2000: ¥4,700,000 (Direct Cost: ¥4,700,000)
Keywordsphloem / sieve tube / companion cell / vascular tissue / transport / gene expression
Research Abstract

Phloem is a route for long distance transport of metabolites and signals in higher plants. Sieve elements, the individual cell components of sieve tubes, are highly specialized for assimilate translocation, and lose most of their intracellular organelles, such as nuclei, vacuoles, Golgi bodies, and most ribosomes during the course of differentiation. However, proteins are thought to be required for the maintenance of the physiological functions of sieve tubes, and are presumed to be supplied from neighboring companion cells through plasmodesmata. Proteins detected in sieve element-companion cell complexes have been categorized by function, such as structural proteins, maintenance proteins involved in redox regulation, proteins related to signal transduction, and others (Hayashi et al. 2000 for review). However, most of the soluble proteins detected in phloem sap have not yet been identified or characterized.
In this experiments, we identified a rice sieve-tube protein with a molecular mass of 31 kDa, RPP31, as a glutathione S-transferase (GST). And we analyzed the detailed chemical composition of rice phloem sap together with the gene expressions in the vascular tissues such as, thoredoxtn h, GST, GS, GOGAT etc..

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Hayashi et al.: "Proteins in the sieve element-companion cell complexes ; their detection, localization and possible functions"Aust.J.Plant Physiol.. 27. 489-496 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kuzuhara et al.: "Glutathione levels in phloem sap of rice plants under sulfur deficient conditions"Soil Sci.Plant Nutr.. 46. 265-270 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saze et al.: "Thioredoxin mediated reductive activation of a protein kinase for the regulatory phosphorylation of C4-form phosphoenolpyruvate carboxylase from maize"Plant Cell Physiol.. 42. 1295-1302 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamaya et al.: "Genetic manipulation and quantitative-trait loci mapping for nitrogen recycling in rice"J.Exp.Botany. 53. 917-925 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suenaga et al.: "Constitutive expression of a novel-type ammonium transporter OsAMT2 in rice plants"Plant Cell Physiol.. 44. 206-211 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hayakawa et al.: "Organization and structure of ferredoxin-dependent glutamate synthase gene from rice plants"Plant Biotechnol.. (In press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tanaka et al.: "Cadmium concentrations in the phloem sap of rice plants (Oryza sativa L.) treated with a nutrient solution containing cadmium"Soil Sci.Plant Nutr.. 49. 311-313 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 林 浩昭: "維管束の構造と物質の輸送"植物細胞工学(秀潤社). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hayashi H. et al.: "Proteins in the sieve element-companion cell complexes ; their detection, localization and possible functions"Aust. J Plant Physiol.. 27. 489-496 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kuzuhara et al.: "Glutathione levels in phloem sap of rice plants under sulfur deficient conditions"Soil Sci. Plant Nutri.. 46. 265-270 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saze et al.: "Thioredoxin mediated reductive activation of a protein kinase for the regulatory phosphorylation of C4-form phosphoenolpyruvate carboxylase from maize"Plant Cell Physiol.. 42. 1295-1302 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamaya et al.: "Genetic manipulation and quantitative-trait loci mapping for nitrogen recycling in rice"J. Exp. Bot. 53. 917-925 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suenaga et al.: "Constitutive expression of a novel-type ammonium transporter OsAMT2 in rice plants"Plant Cell Physil.. 44. 206-211 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hayakawa et al.: "Organization and structure of ferredoxin-dependent glutamate synthase gene from rice plants"Plant Biotechnol.. In press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tanaka et al.: "Cadmium concentrations in the phloem sap of rice plants (Oryza saliva L.) treated with a nutrient solution containing"Soil Sci. Plant Nutr.. 49. 311-313 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hayashi et al.: "Proteins in the sieve element-companion cell complexes ; their detection, localization and Possible functions"Aust. J. Plant Physiol.. 27. 489-496 (2000)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ishiwatari et al.: "In situ hybridization study of the rice phloem thioredoxin h mRNA -possible involvement in the differentiation of vascular tissues"Physiologia Plantarum. 109. 90-96 (2000)

    • Related Report
      2002 Annual Research Report
  • [Publications] Saze et al.: "Thioredoxin mediated reductive activation of a protein kinase for the regulatory phosphorylation of C4-form phosphoenolpyruvate carboxylase from maize"Plant Cell Physiol.. 42. 1295-1302 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamaya et al.: "Genetic manipulation and quantitative-trait loci mapping for nitrogen recycling in rice"J. Exp. Botany. 53. 917-925 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suenaga et al.: "Constitutive expression of a novel-type ammonium transporter OsAMT2 in rice Plants"Plant Cell Physiol.. 44. 206-211 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hayakawa et al.: "Organization and structure of ferredoxin-dependent glutamate synthase gene from rice plants"Plant Biotechnol.. (In press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tanaka et al.: "Cadmium concentrations in the phloem sap of rice plants (Oryza sativa L. ) treated with a nutrient solution containing cadmium"Soil Sci. Plant Nutr.. (In press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 林 浩昭: "植物細胞工学 分担執筆 維管束の構造と物質の輸送"秀潤社. 7 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamaya, et al.: "Genitic maniputlation and quantitative-trait loci mapping for nitrogen recycling in rice"J. Exp. Bot. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Saze, et al.: "Thioredoxin mediated reductive activation of a protein kinase for the regulatory phosphorylation of C4-form phosphoenolpyruvate carboxylase from maize"Plant Cell Physioligy. 42・12. 1295-1302 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hayashi et al.: "Proteins in the sieve element-companion cell complexes ; their detection, localization and possible functions."Aust.J.Plant Physiol.. 27・6. 489-496 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 林浩昭: "肥料の新しい発展を求めて 分担 植物栄養応答反応に関する我々の最近の研究"肥料同人. 8 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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