| Project/Area Number |
12460037
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | University of Tsukuba |
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Principal Investigator |
YAMANE Kunio Tsukuba University, Institute of Biological Sciences, Professor, 生物科学系, 教授 (20013336)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Kouji Tsukuba University, Institute of Biological Sciences, Associate Professor, 生物科学系, 助教授 (40212097)
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| Project Period (FY) |
2000 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥10,700,000 (Direct Cost: ¥10,700,000)
Fiscal Year 2003: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2002: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2001: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2000: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | Bacillus subtilis / Protein secretion machinery / SRP / SecA / Membrane proteins / Proteome / タンパク質分泌機構 / on membrane digestion法 / PVDFメンフレン / MARDI-TOF MS / 分泌タンパク質 / タンパク質の局在化 / ネットワーク / シグナルペプチド |
|---|
| Research Abstract |
Bacillus subtilis and related bacteria has the ability to produce high levels of extracellular enzymes and they have been used as producers of the enzymes in industries. To analyze the molecular mechanism for the protein secretion machinery to produce the enzymes and a possible network between the protein secretion and the localization of proteins in the cell membrane, we have studied as follows
1)Protein composition and function of each component of signal recognition particle (SRP) that targets the presecretory proteins to the cell membrane
2)Interaction between FtsY, a receptor of SRP, and Ffh, a component of SRP
3)Comprehensive analysis of membrane proteins and their relation to the protein secretory machinery to localize the proteins into the cell membrane
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