• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Immunocytochemistry on deposition and degradation of cell wall components

Research Project

Project/Area Number 12460077
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 林産学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

TAKABE Keiji  Graduate School of Agriculture Associate Professor, 農学研究科, 助教授 (70183449)

Co-Investigator(Kenkyū-buntansha) AWANO Tatsuya  Graduate School of Agriculture Assistant Professor, 農学研究科, 助手 (40324660)
YOSHINAGA Arata  Graduate School of Agriculture Assistant Professor, 農学研究科, 助手 (60273489)
FUJITA Minoru  Graduate School of Agriculture Professor, 農学研究科, 教授 (60026599)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥16,100,000 (Direct Cost: ¥16,100,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2001: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2000: ¥9,700,000 (Direct Cost: ¥9,700,000)
Keywordslignification / immunocytochemistry / PAL / C4H / F5H / CAD / SAD / Laccase / 細胞壁 / 抗体 / 木質化 / キシラン / グルコマンナン / 免疫標識 / 分化中木部 / 付加的堆積 / 挿入的堆積
Research Abstract

Immunocytochemical localization of phenylalanine ammonia-lyase(PAL), cinnamate 4-hydroxylase(C4H), ferulate 5-hydroxylase(F5H), cinnamyl alcohol dehydrogenase(CAD), and sinapyl alcohol dehydrogenase(SAD) were investigated in poplar. In addition, localization of laocase(LAC) was observed. Antibodies against the above enzymes were raised using the peptides synthesized according to their amino acid sequences. PAL, F5H, CAD and SAD were localized in the cyto-sol during the secondary wall formation. However, C4H and F5H were localized on the Golgi apparatus and rough endoplasmic reticulum during the secondary wall formation. These results indicate that the hydroxylation of phenylpropane unit occurs in/on the Golgi apparatus and rough endoplasmic reticulum.
Immunolocalization of laccase(LAC) was localized in the later stage of secondary wall formation. Intense labeling was observed at the inner wall of ray parenchyma. LAC may be involved in not only the dehydrogenative polymerization of monolignols but also the protection against the wounding and the microbial attacks
Immunolacalization of xylan and glucomannan was investigated in Japanese beech and Japanese cypress. Xylan is deposited intussusceptionally to the secondary wall, though glucomannan is deposited appositionally.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] T.Awano, K.Takabe, M.Fujita, G.Daniel: "Deposition of glucuronoxylan on the secondary wall of Japanese Beech as observed by immuno-scanning electron microscopy"Protoplasma. 212. 72-79 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Maeda, T.Awano, K.Takabe, M.Fujita: "Immunolocalization of glucomannan in the cell wall of differentiating tracheids in Chamaecynaris obtusa"Protoplasma. 213. 148-156 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Takeuchi, K.Takabe, M.Fujita: "Immunolocalization of O-methyltransferase and peroxidase in the differentiating xylem of poplar"Holzforschung. 55. 146-150 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Takabe, M.Takeuchi, T.Sato, M.Ito, M.Fujita: "Immunocytochemical localization of enzymes involved in lignification of the cell wall"Journal of Plant Research. 114. 509-515 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] C.Tokoh, K.Takabe, J.Sugiyama, M.Fujita: "CP/MAS 13C NMR and electron diffraction study of bacterial cellulose structure affected by cell wall polysaccharides"Cellulose. 9. 351-360 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] I.Duchesne, K.Takabe, G.Daniel: "Ultrastructural Localisation of glucomannan in kraft pulp fibres"Holzforschung. 57. 62-68 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Takabe: "WOOD FORMATION IN TREES"TAYLOR & FRANCIS. 364 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Awano, K. Takabe, M. Fujita and G. Daniel: "Deposition of glucuronoxylan on the secondary wall of Japanese Beech as observed by immuno-scanning electron microscopy"Protoplasma. 212. 72-79 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y. Maeda, T. Awano, K. Takabe and M. Fujita: "Immunolocalization of glucomannan in the cell wall of differentiating tracheids in Chamaecyparis obtusa"Protoplasma. 213. 148-156 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Takeuchi, K. Takabe and M. Fujita: "Immunolocalization of O-methyltransferase and peroxidase in the differentiating xylem of poplar"Holzforschung. 55. 146-150 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K. Takabe, M. Takeuchi, T. Sato, M. Ito and M. Fujita: "Immunocytochemical localization of enzymes involved in lignification of the cell wall"Journal of Plant Research. 114. 509-515 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] C. Tokoh, K. Takabe, J. Sugiyama and M. Fujita: "CP/MAS 13C NMR and electron diffraction study of bacterial cellulose structure affected by cell wall polysaccharides"Cellulose. 9. 351-360 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] I. Duchesne, K. Takabe and G. Daniel: "Ultrastructural Localisation of glucomannan in kraft pulp fibres"Holzforschung. 57. 62-68 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K. Takabe: "WOOD FORMATION IN TREES"TAYLOR & FRANCIS. 364. (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] C.Tokoh, K.Takabe, J.Sugiyama, M.Fujita: "Cellulose synthesized by Acetobacter xylinum in the presence of plant cell wall nolyaccharides"Cellulose. 9. 65-74 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] C.Tokoh, K.Takabe, J.Sugiyama, M.Fujita: "CP/MAS 13C NMR and electron diffraction study of bacterial cellulose structure affected by cell wall nolysaccharides"Cellulose. 9. 351-360 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] C Zhang, M.Fujita, K.Takabe: "Extraction and analysis of the tangential arrangement of cambial cells in Japanese hardwood species"Journal of Wood Science. 48. 353-358 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] I.Duchesne, K.Takabe, G.Daniel: "Ultrastructural Localisation of glucomannan in kraft pulp fibres"Holzforschung. 57. 62-68 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 北川結希, 粟野達也, 高部圭司, 藤田稔: "高分解能走査電子顕微鏡による樹木細胞観察のための試料調整法"木材学会誌. 48・5. 387-391 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Miyuki Takeuchi, Keiji Takabe, Minoru Fujita: "Immunolocalization of O-Methyltransferase and Peroxidase in Differentiating Xylem of Poplar"Holzforschung. 55. 146-150 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Kato, Y.Sakuma, T.Azuma, A.Ando, K.Miura, K.Takabe, Y.Tamai: "Isolation and characterization of a novel anionic peroxidase cDNA found in poplar suspension cultured cells"Journal of Wood Science. 47. 165-170 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Takabe, M.Takeuchi, T.Sato, M.Ito, M.Fujita: "Immunocytochemical Localization of Enzymes Involved in Lignification of the Cell Wall"Journal of Plant Research. 114. 509-515 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] C.Tokoh, K.Takabe, J.Sugiyama, M.Fujita: "Cellulose synthesized by Acetobacter xylinum in the presence of plant cell wall polysaccharides"Cellulose. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Keiji TAKABE: "WOOD FORMATION IN TREES"Taylor & Francis Inc.. 364 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Awano,K.Takabe,M.Fujita and G.Daniel: "Deposition of glucuronoxylans on the secondary wall of Japanese beech as observed by immuno-scanning electron microscopy"Protoplasma. 212. 72-79 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Maeda,T.Awano,K.Takabe and M.Fujita: "Immunolocalization of glucomannans in the cell wall of differentiating tracheids in Chamaecyparis obtusa"Protoplasma. 213. 148-156 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] J.Nakashima,K.Takabe,M.Fujita and H.Fukuda: "Autolysis during in vitro tracheary element differentiation : Formation and location of perforation"Plant and Cell Physiology. 41・11. 1267-1271 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Takeuchi,K.Takabe and M.Fujita: "Immunolocalization of O-methyltransferase and peroxidase in defferentiating xylem of poplar"Holzforschung. (in press). (2001)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2000-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi