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Taxonomic, phylogenic and breeding studies of Pleurotus mushrooms and their application to bioremediation

Research Project

Project/Area Number 12460079
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 林産学
Research InstitutionTOTTORI UNIVERSITY

Principal Investigator

KITAMOTO Yutaka  Tottori Univ., Fac. of Agr., Prof., 農学部, 教授 (10032294)

Co-Investigator(Kenkyū-buntansha) MORINAGA Tsutomu  Hiroshima Pref. Univ., Fac. of Biosci., Prof., 生物資源学部, 教授 (40034417)
HIGAKI Miyato  Tokyo Agr. Univ., Fac. of Environ., Prof., 地域環境学部, 教授 (50078143)
KUWAHARA Masaaki  Kyoto Univ., Inst. Wood Sci., Prof., 木質研, 教授 (40035978)
OHGA Shoji  Kyushu Univ., Fac. of Agr., Assoc. Prof., 農学部, 助教授 (60117075)
WARIISHI Hiroyuki  Kyushu Univ., Fac. of Agr., Assoc. Prof., 農学部, 助教授 (50253513)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 2001: ¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 2000: ¥7,800,000 (Direct Cost: ¥7,800,000)
Keywordsbasidiomycetes / Pleurotus spp. / taxonomy / phylogeny / bioremediation / manganese peroxidase / Protoplast / genetic engineering / ラッカーゼ
Research Abstract

Taxonomic, phylogenic and genetic studies of Pleurotus species were carried out with use of 24 strains collected worldwide. They were divided into 5 subspecies groups and 9 independent species by the mating test. The strains belonging to each subspecies showed the same results by the RFLP analysis of 26S rDNA, and the phylogenic tree by the DNA analysis was prepared for the strains tested. We demonstrated that the sequence analysis for a region of ITS1-5.8S-ITS2 of rDNA was useful for identification of species of Pleurotus mushrooms. We established a pluralistic method for determination of species of Pleurotus mushrooms with use of the mating test and the DNA analysis. The linkage of P. ostreatus 9 chromosomes has been prepared.
We produced several superior commercial strains of Pleurotus mushrooms by crossing among the strains belonging to each subspecies group as described above. We selected a protoclone that showed very high activity for lignin biodegradation. We established a molecular breeding method for Pleurotus mushrooms by developing the transformation system with use of Carboxin and Hygromycin B resistance as selective markers. New substrates and improved methods for cultivation of Pleurotus mushrooms have been also accomplished.
Bioremediation studies revealed that manganese peroxidase which was contained in Pleurotus mushrooms could degrade bis-pohenol A (BSA). We demonstrated the metabolic pathways for the degradation of herbicides (CNP and NIP) by Coriolus versicolor, and showed the existence of a similar pathway in P. ostreatus. The cytochrome P450-cooperated biodegradation activity for diphenyl ether was found in wood rotting basidiomycetes.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Irie, T., Honda, Y., Ha, H-C., Watanabe, T., Kuwahara, M.: "Isolation of cDNA and genomic fragment eacoding the major manganase peroxidase isozyme from the white rot basidiomycete Pleurotus ostreatus"J.Wood Sci. 46. 230-233 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Honda, Y., Matsuyama, T., Irie, T., Watanabe, T., Kuwahara, M.: "Carboxin resistance transformation of the homobasidiomycete fungus Pleurot us ostreatus"Curr. Genet.. 37. 209-212 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirano, T., Honda, Y., Watanabe, T., Kuwahara.M.: "Degradation of bisphenol A (BPA) by the lignin-degrading enzyme, manganase peroxidase, produced by the white-rot basidiomycete, Pleurotus ostreatus"Biosci.Biotechnol.Biochem.. 64. 1958-1962 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Irie, T., Honda, Y., Watanabe, T., Kuwahara.M.: "Efficient transformation of filamentous fungus Pleurotus ostreatus using single-strand carrier DNA."Appl. Microbiol. Biotechnol.. 55. 563-565 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Irie, T., Honda, Y., Watanabe, T., Kuwahara.M.: "Homologous expression of recombinant manganese peroxidase genes in ligno-lytic fungus Pleurotus ostreatus"Appl. Microbiol. Biotechnol.. 55. 566-570 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Irie, T., Honda, Y., Hirano, T., Sato.T., Enei, H., Watanabe.T., Kuwahara, M.: "Stable transformation of basidiomycete Pleurotus ostreatus to hygromycin B resistance using Lentinus edodes GPD expression signals"Appl. Microbiol. Biotechnol.. 56. 707-709 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiratsuka, N., Wariishi, H., Tanaka, H.: "Degradation of diphenyl ether herbicides by the lignin-degradinig basidiomycete Coriolus versicolor"Appl. Microbiol. Biotechnol.. 57. 563-571 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ichinose, H., Wariishi, H., Tanaka, H.: "Identification and characterization of novel cytochrome P450 genes from white-rot basidiomoycete Coriolus versicolor"Appl. Microbiol. Biotechnol.. 58. 97-105 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ichinose, H., Nakamizo, M., Wariishi, H., Tanaka, H.: "Metabolic response against sulfur-containing heterocyclic compounds by lignin-degrading basidiomycete Coriolus versicolor"Appl. Microbiol. Biotechnol.. (印刷中). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiratsuka, N., Wariishi, H., Tanaka, H.: "Degradation of diphenyl ether herbicides by the lignin-degrading basidiomycete Coriolus versicolor"Appl. Microbiol. Biotechnol.. 57. 563-571 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ichinose, H., Wariishi, H., Tanaka, H.: "Identification and characterization of novel cytochrome P450 genes from white-rot basidiomycete Coriolus versicolor"Appl. Microbiol. Biotechnol.. 58. 97-105 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Honda, Y., Irie, T., Watanabe, T., Kuwahara, M.: "Molecular breeding of the oyster mushroom using a homologous DNA-mediated transformation"Science and Cultivation of Edible Fungi (ed.L.J.L.D. Griensven) Balkeima, Rotterdam. 151-156 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Irie, T. et al.: "Isolation of cDNA and genomic fragment encoding the major manganase peroxidase isozyme from the white rot basidiomycete Pleurotus ostreatus"J. Wood Sci.. 46. 230-233 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Honda, Y. et al.: "Carboxin resistance transformation of the homobasidiomycete fungus Pleurotus ostreatus"Curr. Genet.. 37. 209-212 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirano, T. et al.: "Degradation of bisphenol A (BPA) by the lignin-degrading enzyme, manganase peroxidase, produced by the white-rot basidiomycete, Pleurotus ostreatus"Biosci. Biotechnol. Biochem. 64. 1958-1962 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Irie, T. et al.: "Efficient transformation of filamentous fungus Pleurotus ostreatus using single-strand carrier DNA"Appl. Microbiol. Biotechnol.. 55. 563-565 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Irie, T. et al.: "Homologous expression of recombinant manganese peroxidase genes in ligno-lytic fungus Pleurotus ostreatus"Appl. Microbiol. Biotechnol.. 55. 566-570 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Irie, T. et al.: "Stable transformation of basidiomycete Pleurotus ostreatus to hygromysin B resistance using Lentinus edodes GPD expression signals"Appl. Microbiol. Biotechnol.. 56. 707-709 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiratsuka, N. et al.: "Degradation of diphenyl ether herbicides by the lignin-degrading basidio-mycete Coriolus versicolor"Appl. Microbiol. Biotechnol.. 57. 563-571 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ichinose, H. et al.: "Identification and characterization of novel cytochrome P450 genes from white-rot basidiomycete Coriolus versicolor"Appl. Microbiol. Biotechnol.. 58. 97・1051 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ichinose, H. et al.: "Metabolic response against sulfur-containing heterocyclic compounds by lignin-degrading bacidiomycete Coriolus versicolor"Appl. Microbiol. Biotechnol.. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Honda, Y. et al.: "Molecular breeding of the oyster mushroom using a homologous DNA-mediated transformation. In Science and Cultivation of Edible Fungi (ed. L.J.L.D. Griensven)"Balkema, Rotterdam. 151-156 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Irie, T., Honda, Y., Watanabe, T., Kuwahara, M.: "Efficient transformation of filamentous fungus Pleurotus ostreatus using single-strand carrier DNA"Appl. Microbiol. Biotechnol.. 55. 563-565 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Irie, T., Honda, Y., Watanabe, T., Kuwahara, M.: "Homologous expression of recombinant manganese peroxidase genes in lignolytic fungus Pleurotus ostreatus"Appl. Microbiol. Biotechnol.. 55. 566-570 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Irie, T., Honda, Y., Hirano, T., Sato, T., Enei, IH., Watanabe, T., Kuwahara, M.: "Stable transformation of basidiomycete Pleurotus ostreatus to hygromycin B resistance using Lentinus edodes GPD expression signals"Appl. Microbiol. Biotechnol.. 56. 707-709 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hiratsuka, N., Wariishi, H., Tanaka, H.: "Degradation of diphenyl ether herbicides by the lignin-degrading basidiomycete Coriolus versicolor"Appl. Microbiol. Biotechnol.. 57. 563-571 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ichinose, H., Wariishi, H., Tanaka, H.: "Identification and characterization of novel cytochrome P450 genes from white-rot basidiomycete Coriolus versicolor"Appl. Microbiol. Biotechnol.. 58. 97-105 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ichinose.H., Nakamizo, M., Wariishi, H., Tanaka, H.: "Metabolic response against sulfur-containing heterocyclic compounds by lignin-degrading basidiomycete Coriolus versicolor"Appl. Microbiol. Biotechnol.. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Honda, Y., Irie, T., Watanabe, T., Kuwahara, M.: "Science and Cultivation of Edible Fungi (ed. L.J.L.D. Griensven) Balkerma, Rotterdam"Molecular breeding of the oyster mushroom using a homologous DNA-Mediated transformation. 151-156 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Irie,T.,Honda,Y.,Ha.H-C.,Watanbe,T.and Kuwahara,M.: "Isolation of cDNA and genomic fragments encoding the major manganase peroxidase isozyme from the white rot basidiomycete, Pleurotus ostreatus"J.Wood Sci.. 46. 230-233 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Honda,Y.,Matsuyama,T.,Irie,T.,Watanabe,T.and Kuwahara,M.: "Carboxin resistance transformation of the homobasidiomycete fungus Pleurotus ostreatus"Curr.Genet.. 37. 209-212 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Olesnicky,N.S.,Brown,A.J.,Honda,Y.,Dyps,S.L.,Dowell,S.J.and Casselton,L.A.: "Self-compatible B mutants in Coprinus with altered pheromone-receptor specificities."Genetics. 156. 1025-1033 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nogami,T.,Yamasaki,Y.,Suzuki,A.and Kitamoto,Y.: "Physiological characteristics on the germination of sexual and asexual spores from various wood rotting basidiomycetous mushrooms"日本応用きのこ学会誌. 9. (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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