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Role of apoptosis (programmed cell death) in fish sex differentiation

Research Project

Project/Area Number 12460085
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionTokyo University of Fisheries

Principal Investigator

STRUSSMANN Carlos a  Tokyo University of Fisheries, Faculty of Fisheries, Associate Professor, 水産学部, 助教授 (10231052)

Co-Investigator(Kenkyū-buntansha) SAKAMOTO Takashi  Tokyo University of Fisheries, Faculty of Fisheries, Assistant Professor, 水産学部, 助手 (40313390)
YOSHIZAKI Goro  Tokyo University of Fisheries, Faculty of Fisheries, Assistant Pr。fessor, 水産学部, 助手 (70281003)
YAMASHITA Hichiaki  National Research Institute of Fisheries Science, Senior Researcher Tokyo University of Fisheries, Faculty of Fisheries, Associate Professor, 水産学部, 助教授
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥9,000,000 (Direct Cost: ¥9,000,000)
Fiscal Year 2002: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2001: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥3,400,000 (Direct Cost: ¥3,400,000)
KeywordsSEX DIFFERENTITION / APOPTOSIS / GENETIC SEX MARKER / GERM CELL MARKER / FISH / STEROID HORMONE / PEJERRY / ZEBRAFISH / AFLP / GnRH / ニジマス
Research Abstract

Apoptosis, or programmed cell death, is a process of cell death which is implicated in the natural ontogenesis and differentiation of tissues as well as the elimination of cells that have been irreversibly damaged by environmental stress, including high temperatures. This research revealed that apoptosis is involved in the gonadal sex differentiation of some fish species, particularly those in which the gonads pass through an ambisexual (intersex) stage prior to actual differentiation into ovaries and testes. For example, in ie zebrafish, the degeneration and disappearance of female germinal and somatic elements from the gonads of the genetic males during testicular differentiation was shown to be through apoptosis. We also elucidated the ability of various environmental factors to induce apoptosis in fish embryos and characterized the molecular cascade leading to cellular apoptosis. Ln species with thermolabile sex differentiation (TSD) such as pejerrey, it was shown that apoptosis oc … More curs in the gonads of larvae subjected to heat-stress and, therefore, that this is a plausible mechanism to explain sex determination at different temperatures. Fish germ cells were found to be particularly sensitive to heat. Meanwhile, we examined other molecular, biochemical and cellular pathways that could act in concert with apoptosis to induce gonadal sex differentiation. These included the differential expression of P450c aromatase, a steroidogenic enzyme necessary for the production of estrogen, and the expression of estrogen receptors α and β genes at different temperatures in pejerrey. This study also revealed that brain (GnRH) and pituitary (GtH1 and GtH2) hormones are produced already before sex differentiation and show differential abundance at feminizing and masculinizing temperatures, suggesting a role in the regulation of gonadal steroidogenesis during TSD. Ln addition, we developed methods to distinguish germ cells form somatic cells using the vasa gene promoter in rainbow trout and gilthead sea bream as wd as sex linked markers to identify the genotypic sex in rainbow trout and pejerrey. Less

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (50 results)

All Other

All Publications (50 results)

  • [Publications] L.S.Ito et al.: "Histological process and dynamics of germ cell degeneration in pejerrey Odontesthes bonariensis larvae and juveniles during exposure to warm water"Journal of Experimental Zoology. 293. 492-499 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 山下倫明: "ゼブラフィッシュ胚におけるアポトーシス(トランスジェニック体を用いて発生・分化でのアポトーシスの分子機構を探る)"化学と生物. 41. 143-145 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] L.A.Miranda et al.: "Gonadotropin-releasing hormone neuronal development during the sensitive period of temperature sex determination in the pejerrey fish, Odontesthes bonariensis"General and Comparative Endocrinology. (In press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] C.A.Strussmann, M.Nakamura: "Morphology, endocrinology, and environmental modulation of gonadal sex differentiation in teleost fishes"Fish Physiology and Biochemistry. 26. 13-29 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] G.Yoshizaki et al.: "Visualization of primordial germ cells in transgenic rainbow trout carrying green fluorescent protein gene driven by vasa promoter"Fisheries Science. 68S. 1067-1070 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] L.S.Ito et al.: "Dynamics of heat-induced germ cell loss in pejerrey, Odontesthes bonariensis"Fisheries Science. 68S. 1013-1014 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] D.Uchida et al.: "Oocyte apoptosis during the transition from ovary-like tissue to testis during sex differentiation of juvenile zebrafish"Journal of Experimental Biology. 205. 710-718 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Takeuchi et al.: "Mass isolation of primordial germ cells from transgenic rainbow trout carrying the green fluorescent protein gene driven by the vasa gene promoter"Biology of Reproduction. 67. 1087-1092 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Yabu et al.: "Stress-inducted apoptosis by heat shock, UV and gamma-ray irradiation in zebrafish embryos detected by increased caspase activity and whole-mount TUNEL staining"Fisheries Science. 67. 333-340 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] L.A.Miranda et al.: "Immunocytochemical identification of GtH1 and GtH2 cells during the temperature-sensitive period for sex determination in pejerrey, Odontesthes bonariensis"General and Comparative Endocrinology. 124. 45-52 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Yabu et al.: "Characterization of zebrafish caspase-3 and induction of apoptosis through ceramide generation in fish fathead minnow tailbud cells and Zebrafish embryos"Biochemical Journal. 360. 39-47 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] G.Yoshizaki et al.: "Cloning and characterization of a vasa-like gene in rainbow trout and its expression in the germ cell lineage"Molecular Reproduction and Development. 55. 364-371 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Sakamoto et al.: "A microsatellite linkage map of rainbow trout (Oncorhynchus mykiss) characterized by large sex-specific differences in recombination rates"Genetics. 155. 1331-1345 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] G.Yoshizaki et al.: "Germ cell-specific expression of green fluorescent protein in transgenic rainbow trout under control of the rainbow trout vasa-like gene promoter"International Journal of Developmental Biology. 44. 323-326 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Yamashita et al.: "Stress response and apoptosis in zebrafish embryos"Aquatic Genomics -Steps toward a great future (N. Shimizu, T. Aoki, I. Hirono, F. Takashima, eds.) Springer Verlag, Tokyo. 195-206 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] C.A.Strussmann et al.: "Methods of sex control in fishes and an overview of novel hypotheses concerning the mechanisms of sex differentiation"Fish Genetics and Aquaculture Biotechnology (T.J. Pandian, C.A. Strussmann, M.P. Marian, eds.) Oxford & IBH Publishing (New Delhi, India) & Science Publishers (Enfield, New Hampshire, USA). (In press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Cardinali et al.: "Cloning, characterization and expression of vasa-like gene in the gilthead sea bream"Perspective in comparative endocrinology : unity and diversity (H.J.Th Goos, R.K. Rastogi, H. Vaudry, R. Pierantoni, eds) Monduzzi Editore, Bologna (Italy). 197-203 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] L.S.Ito, M.Yamashita and C.A.Strussman: "Historical process and dynamics of germ cell degeneration in pejerrey Odontesthes bonariensis lavae and juveniles during exposure to warm water"Journal of Experimental Zoology. 293. 492-499 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] L.A.Miranda: "Strobl-Mazzulla, P.H., C.A. Strussmann, I. Parhar and G.M. Somoza Gonadotropin-releasing hormone neuronal development during the sensitive period of temperature sex determination in the pejerrey fish, Odontesthes bonoriensis"General and Comparative Endocrinology. (in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] C.A. Strussmann and M Nakamura: "Morphology, endocrinology, and environmental modulation of gonadal sex differentiation in teleost fishes"Fish Physiology and Biochemistry. 26. 13-29 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] G. Yoshizaki, Y. Takeuchi, H. Tominaga, T. Kobayashi and T. Takeuchi: "Visualization of primordial germ cels in transgenic rainbow trout carrying green fluorescent protein gene driven by vasa promoter"Fisheries Science. 68S. 1067-1070 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] L.S. Ito, M. Yamashita and C.A. Strussmann: "Dynamics of heat-induced germ cell loss in pejerrey, Odontesthes bonariensis"Fisheries Science. 68S. 1013-1014 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Uchida, M. Yamashita, T. Kitano and T. Iguchi: "Oocyte apoptosis during the transition from ovary-like tissue to testis during sex differentiation of juvenile zebrafish"Journal of Experimental Biology. 205. 710-718 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y. Takeuchi, G. Yoshizaki, T. Kobayashi and T. Takeuchi: "Mass isolation of primordial germ cells from transgenic rainbow trout carrying the green fluorescent protein gene driven by the vasa gene promoter"Biology of Reproduction. 67. 1087-1092 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Yabu, S. Todoriki and M. Yamashita: "Stress-inducted apoptosis by heat shock, UV and gamma-ray irradiation in zebrafish embryos detected by increased caspase activity and whole-mount TUNEL staining"Fisheries Science. 67. 333-340 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] L.A. Miranda, C.A. Strussmann and G.M. Somoza: "Immunocytochemical identification of GtH1 and GtH2 cells during the temperature-sensitive period for sex determination in prejerry,Odontesthes bonariensis"General and Comparative Endocrinology. 124. 45-52 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Yabu, S. Kishi, T. Okazaki and M. Yamashita: "Characterization of zebrafish caspase-3 and induction of apoptosis through ceramide generation in fish fathead minnow tailbud cells and zebrafish embryos"Biochemical Journal. 360. 39-47 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] G. Yoshizaki, S. Sakatani, H. Tominaga and T. Takeuchi: "Cloning and characterization of a vasa-like gene in rainbow trout and its expression in the germ cell lineage"Molecular Reproduction and Development. 55. 364-371 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Sakamoto, R.G. Danzmann, K. Gharbi, P. Howard, A. Ozaki, S.-K. Khoo, R.A. Woram, N. Okamoto, M.F. Ferguson, L.-E.Holm,R. Guyomard and B. Hoyheim: "A microsatellite linkage map of rainbow trout (Oncorhynchus mykiss) characterized by large sex-specific differences in recombination rates"Genetics. 155. 1331-1345 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] G. Yoshizaki, Y. Takeuchi, S. Sakatani and T. Takeuchi: "Germ cell-specific expression of green fluorescent protein in transgenic rainbow trout under control of the rainbow trout vasa-like gene promoter"International Journal of Developmental Biology. 44. 323-326 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Yamashita, K. Uchino, Y. Taguchi, S. Imamura, D. Uchida, T. Yabu, M. Hojo and N. Ojima , eds. by N. Shimizu, T. Aoki, I. Hirono,and F, Takashima: "Stress response and apoptosis in zebrafish embryos In : Aquatic Genomics - Steps toward a great future"Springer Verlag. 195-206 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] C.A. Strussmann, M. Karube, L.A. Miranda, R. Patifto, G.M. Somoza, D. Uchida and M. Yamashita , eds. by T.J. Pandian, C.A. Strussmann and M.P. Marian: "Methods of sex control in fishes and an overview of novel hypotheses concerning the mechanisms of sex differentiation In : Fish Genetics and Aquaculture Biotechnology"Oxford & IBM Publishing (New Delhi, India) & Science Publishers (Enfield, New Hampshire, USA)(in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Cardinali, M. Mancini, A.M Polzonetti-Magni, G. Yoshizaki and O. Carnevali , eds. by H.J.Th Goos, R.K. Rastogi, H. Vaudry and R. Pierantoni: "Cloning, characterization and expression of vasa-like gene in the gilthead sea bream In : Perspectives in comparative endocrinology: unity and diversity"Monduzzi Editore. 197-203 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] C.A.Strussmann, M Nakamura: "Morphology, endocrinology, and environmental modulation of gonadal sex differentiation in teleost fishes"Fish Physiol.Biochem.. (In press).

    • Related Report
      2002 Annual Research Report
  • [Publications] L.S.Ito et al.: "Histological process and dynamics of germ cell degeneration in pejerrey Odontesthes bonariensis larvae and juveniles during exposure to warm water"J.Exp.Zool.. (In press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Miranda, L.A. et al.: "Gonadotropin-releasing hormone neuronal development during the sensitive period of temperature sex determination in the pejerrey fish, Odontesthes bonariensis"Gen.Comp.Endocrinol. (In press).

    • Related Report
      2002 Annual Research Report
  • [Publications] D.Uchida et al.: "Oocyte apoptosis during the transition from ovary-like tissue to testis during sex differentiation of juvenile zebrafish"J.Exp.Biol.. 205. 710-718 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Yabu et al.: "Stress-inducted apoptosis by heat shock, UV and gamma-ray irradiation in zebrafish embryos detected by increased caspase activity and whole-mount TUNEL staining"Fisheries Science. 67. 333-240 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Yabu et al.: "Characterization of zebrafish caspase-3 and induction of apoptosis through ceramide generation in fish fathead minnow tailbud cells and zebrafish embryos"Biochem.J.. 360. 39-47 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Yamashita et al.: "Aquatic Genomics (ed. by Aoki et al.) (Chapter title : Stress response and apoptosis in zebrafish embryos)"Springer Verlag(In press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Ito, L.S. et al.: "Dynamics of heat-induced germ cell loss in pejerrey, Odontesthes bonariensis"Proceedings of the International Commemorative Symposium of the 70^<th> Anniversary of the Japanese Society of Fisheries Science, Yokohama, Japan. (In press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takeuchi, Y. et al.: "Production of germ-line chimeras in rainbow trout by blastomere transplantation"Molecular Reproduction and Development. 59. 380-389 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoshizaki, G. et al.: "Visualization of primordial germ cells in transgenic rainbow trout carrying green fluorescent protein gene driven by vasa promoter"Proceedings of the International Commemorative Symposium of the 70^<th> Anniversary of the Japanese Society of Fisheries Science, Yokohama, Japan. (In press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Miranda, L.A. et al.: "Immunocytochemical identification of GtH1 and GtH2 cells during the temperature-sensitive period for sex determination in pejerrey, Odontesthes bonariensis"General and Comparative Endocrinology. (In press). (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Cardinali M. et al.: "Perspective in comparative endocrinology : unity and diversity (Ed. by Goos H.J.Th, Rastogi, R.K., Vaudry H., and Pierantoni R.)"Cloning, characterization and expression of vasa-like gene in the gilthead sea bream. 197-203 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takeuchi, Y. et al.: "Aquatic Genomics(Springer)"Visualization and isolation of live primordial germ cells aimed at cell-mediated gene transfer in rainbow trout (In press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sakamoto,T.: "A microsatellite linkage map of rainbow trout (Oncorhynchus mykiss) characterized by large sex-specific differences in recombination rates"Genetics. 155. 1331-1345 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshizaki,G.: "Cloning and characterization of a vasa-like gene in rainbow trout and its expression in the germ cell lineage"Molecular Reproduction and Development. 55. 364-371 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshizaki,G.: "Germ cell-specific expression of green fluorescent protein in transgenic rainbow trout under control of the rainbow trout vasa-like gene promoter"International Journal of Developmental Biology. 44. 323-326 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Strussmann,C.A.: "Proceedings of the First International Conference on Advanced Technologies in Fisheries and Marine Sciences,Trivandrum,India"Methods of sex control in fishes and an overview of novel hypotheses concerning the mechanisms of sex differentiation(In press). (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2021-04-07  

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