• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study on the protein digestion and nitrogen metabolism of horses fed high-roughage diets

Research Project

Project/Area Number 12460116
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

TANAKA Keiichi  Hokkaido Univ., Grad. School of Agr., Prof., 大学院・農学研究科, 教授 (30021710)

Co-Investigator(Kenkyū-buntansha) TANIGUCHI Kozo  Hiroshima Univ., Fac. Of Biol. Prod., Prof., 生物生産学部, 教授 (30093777)
KOBAYASHI Yasuo  Hokkaido Univ., Grad. School of Agr., Asso. Prof., 大学院・農学研究科, 助教授 (50153648)
HATA Hiroshi  Hokkaido Univ., Field Scie. Center North. Bio Asso. Prof., 北方生物圏フィールド科学センター, 助教授 (30250492)
OBITSU Takehito  Hiroshima Univ., Fac. Of Biol. Prod., Asso. Prof., 生物生産学部, 助教授 (30194632)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2001: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2000: ¥9,100,000 (Direct Cost: ¥9,100,000)
Keywordshorse / ileum cannulae / dietary protein contents / nitrogen accumulation / ferimentation in hindgut / ^<15>N ammonia nitrogen / free amino acids in plasma / essential amino acids / 回腸末端カニューレ / 飼料中粗タンパク質含量 / 窒素出納 / 窒素蓄積量 / [^<15>N]塩化アンモニウム / 飼料中タンパク質水準 / 尿素代謝 / 粗飼料主体飼養 / タンパク質消化 / 窒素代謝 / 窒素蓄積率 / 血漿中尿素態窒素 / 繊維分解菌
Research Abstract

Hokkaido native horses fitted with the cannulae in the endo of ileum were used in this study.
1) Effect of dietary protein contents on nitrogen metabolism of horses. Urea synthesis in the body and its transfer to digestive tract from the body, and urinary excretion of urea were increased with increasing protein intake. When protein intake was decreaed, most of nitrogen that transferred to digestive tract from the body were utilized for urea re-synthesis. This suggested that urea transfer to digestive tract made a contribution to urea utilization of horses, when protein intake was decreased.
2) Effect of dietary protein contents on feed utilization and fermentation of hindgut of horses. Crude protein digestibility and the rate of protein accumulation was increased with increasing protein intake. Total VFA and NH_3-N concentrations in the hindgut was also increased, whereas pH tended to be decreased. When dietary protein level was increased, protein digestibility in the small intestine was increased. Then, nitrogen flow into the hindgut was increased. NDF and ADF digestibilities, as well as DAPA concentration in urine were not affected by dietary protein levels.
3) Transfer into plasma amino acids from NH_3-N infused into the ileum of horses Ammonia labelled ^<15>N was infused into the ileum of hoses in order to investigate the fate of endogeneous ammonia translated into digestive tract. All bacterial amino acid-N in the colon contained about 1% of ^<15>N-nitrogen. Free essential amino acids in plasma, such as leu, ile, val, his, lys, phe, had higher ^<15>N. This suggested that a part of plasma amino acids originated from amino acids synthesized by bacteria in the colon of horses.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] 小林泰男, 小池聡, 秦寛, 新宮裕子, 田中桂一, 大久保正彦: "ウマ後腸微生物組成における品種および季節間での比較・検討"ルーメン研究会誌. 12. 79-80 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koike, S., Y.Kobayashi, H.Hata, K.Tanaka: "Fecal bacteria in Hokkaido native horses as characterized by microscopic enumeration and competitive PCR assays"Journal of Equine Science. 11. 45-50 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Taniguchi, K.Tanaka 他: "Use of a rigid-type polyethylene T-cannula, silicone tubing, and adhesion netting for surgical cannulation of ileum of the horses"J. Vet. Med. Sci.. (印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koike, Sy., Y. Kobayashi, H. Hata, K. Tanaka, et al.: "Fecal bacteria of Hokkaido native horses as charecterized by microscopic enumerstion and competitive PCR assays."J. Equine Sci.. 11-2. 45-50 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K. Taniguchi, K. Tanaka, et al.: "Use of a rigid type polyethylen T-cannula, silicone tubing, and adhesion nettiny for surgical cannulation of ileum of the horse"J. Vet. Med. Sci.. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Taniguchi, K.Tanaka他: "Use of A Rigid-Type Polyethylene T-Cannula, Silicone Tubing, and Adhesion Netting for Surgical Cannulation of Ileum of the Horse"J.Vet.Med.Sci.. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小林泰雄, 小池聡, 秦寛, 新宮裕子, 田中桂一, 大久保正彦: "ウマ後腸微生物組成における品種および季節間での比較検討"ルーメン研究会. 12・(2). 79-80 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Koike, S., Y. Kobayashi, H. Hata, K. Tananaka他: "Fecal bactenia of Hokkaido native horses as characterized by microscopic enumeration and competitive RCR assays"Journal of Equine Science. 11・(2). 45-50 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Koike, S., Y.Kobayashi, H.Hata, K. Tanaka 他: "Fecal bacteria of Hokkaido mative horses as characterized by microscopic enumeration and Competitive PCR assays"Journal of Equine Science. (印刷中).

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2000-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi