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Change in the host range specificity of herpesvirus by introducing other types of herpesvirus

Research Project

Project/Area Number 12460133
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

OTSUKA Haruki  THE UNIVERSITY OF TOKYO, Graduate School of Agricultural and Life Sciences, Professor (80261957)

Co-Investigator(Kenkyū-buntansha) MATSUMOTO Yasunobu  THE UNIVERSITY OF TOKYO, Graduate School of Agricultural and Life Sciences, Assistant (90251420)
TAKASHIMA Yasuhiro  THE UNIVERSITY OF TOKYO, Graduate School of Agricultural and Life Sciences, Assistant (20333552)
松本 芳嗣  東京大学, 大学院・農学生命科学研究科, 助教授 (00173922)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 2002: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥6,500,000 (Direct Cost: ¥6,500,000)
KeywordsBHV-1 / PRV / TF7-6 / Th1 / apoptosis / UsORF8 / D8 / RK13 / semi-permissive / non-permissive / GFP / Th2 / gB / gC / HmLu-1 / IE gene / green fluorescent protein / A31細胞
Research Abstract

Bovine herpesvirus 1 (BHV-1) has a relatively narrow host cell range in vitro and in vivo when compared to pseudorabies virus (PRV). The aim of this study was to elucidate whether homologous glycoproteins gC and gB from PrV can function in a heterologous BHV-1 background and whether the expression of these PRV glycoproteins influences the in vitro host cell specificity of BHV-1. We constructed BHV-1 recombinant, BHV-1/TF7-6 in which PRV gC and gB were expressed and examined their attachment and penetration properties in permissive, semi-permissive and non-permissive cells. It was found that the expression of PRV gC and gB in a BHV-1 environment facilitated virus attachment and penetration by BHV-1 to semi- or non-permissive cells. When BALB/c mice were inoculated with purified BHV-1/TF7-6 or the parental BHV-1, the former induced lower level of anti-BHV-1 IgG than the latter did. However, the IgG2a:IgG1 ratio was higher in BHV-1/TF7-6 inoculated mice than in the parental BHV-1 inoculated ones. These results indicate that BHV-1/TF7-6 induced type 1 predominant immunity to BALB/c mice.
BHV-1 Us ORF8 protein with homology to the aUs9 protein of other alphaherpesviruses induces apoptosis in rabbit kidney (RK13) cells without the presence of other BHV-1-encoded proteins. A mutant of BHV-1 that fails to express BHV-1 UsORF8 protein, BHV-1/D8 exhibited a reduced cyto-toxicity to RK13 cells when compared to the cyto-toxicity of the parental BHV-1. In RK13 cells, the onset of apoptosis was not observed during the infection with BHV-1/D8, and the virus replication of BHV-1/D8 was markedly greater than that of control virus. These date appeared to indicate that the Us ORF8 protein activates the apoptosis process and facilitates virus release from BHV-1 infected cells.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (18 results)

All 2002 2001 Other

All Journal Article (12 results) Publications (6 results)

  • [Journal Article] Pseudorabies virus (PRV) is protected from complement attack by cellular factors and glycoprotein C(gC).2002

    • Author(s)
      Maeda, K., Hayashi, S., Tanioka, Y., Matsumoto Y., Otsuka, H.
    • Journal Title

      Virus Res. 84

      Pages: 79-87

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Induction of apoptosis in rabbit kidney cell under high-level expression of bovine Herpesvirus 1 Us ORF8 product2002

    • Author(s)
      Nakamichi, K., Matsumoto, Y., Tohya, Y., Otsuka, H.
    • Journal Title

      Intervirology 45

      Pages: 85-93

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Bovine herpesvirus-1 (BHV-1) recombinant expressing pseudorabies viru (PRV) glycoproteins B and C induces Type 1 immune response in BALB/c mice.2002

    • Author(s)
      Takashima, Y., Nagane, N., Hushur, O., Matsu-Moto, Y., Otsuka, H
    • Journal Title

      J.Vet.Med.Sci. 64

      Pages: 589-596

    • NAID

      110003920920

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Bovine herpesvirus 1 Us ORF8 protein induces apoptosis in infected cells and Facilitates virus egress.2002

    • Author(s)
      Nakamichi, K., Matsumoto, Y., Otsuka, H.
    • Journal Title

      Virology 304

      Pages: 24-32

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Pseudorabies virus (PRV) is protected from complement attack by cellular factors and glycoprotein C (gC).2002

    • Author(s)
      Maeda, K., Hayashi, S., Tanioka, Y., Matsumoto, Y., Otsuka, H.
    • Journal Title

      Virus Res 84

      Pages: 79-78

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Induction of apoptosis in rabbit kidney cell under high-level expression of bovine herpesvirus 1 Us ORF8 product.2002

    • Author(s)
      Nakamichi, K., Matsumoto, Y., Tohya, Y., Otsuka, H.
    • Journal Title

      Intervirology 45

      Pages: 85-93

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Bovine herpesvirus-1(BHV-1) recombinant expressing pseudorabies viru (PRV) glycoproteins B and C in Type 1 immune response in BALB/c mice.2002

    • Author(s)
      Takashima, Y., Nagane, N., Hushur, O., Matsumoto, Y., Otsuka, H.
    • Journal Title

      J.Vet.Med.Sci. 64

      Pages: 589-596

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Bovine herpesvirus 1 Us ORF8 protein induces apoptosis in infected cells and facilitates virus egress.2002

    • Author(s)
      Nakamichi, K., Matsumoto, Y., Otsuka, H.
    • Journal Title

      Virology 304

      Pages: 24-32

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Bovine herpesvirus 1 glycoprotein G is required for prevention of apoptosis and Efficient viral growth in rabbit kidney cells.2001

    • Author(s)
      Nakamichi, K., Kuroki, D., Matsumoto, Y., Otsuka, H
    • Journal Title

      Virology 279

      Pages: 488-498

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Defective infection of bovine herpesvirus 1 in non-permissive murine cells.2001

    • Author(s)
      Nakamichi, K., Matsumoto, Y., Otsuka, H.
    • Journal Title

      J.Vet.Med.Sci. 63

      Pages: 1139-1143

    • NAID

      110003920762

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Bovine herpesvirus 1 glycoprotein G is required for prevention of apoptosis and Efficient viral growth in rabbit kidney cells.2001

    • Author(s)
      Nakamichi, K., Kuroki, D., Matsumoto, Y., Otsuka, H.
    • Journal Title

      Virology 279

      Pages: 488-498

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Defective infection of bovine herpesvirus 1 in non-permissive murine cells.2001

    • Author(s)
      Nakamichi, K., Matsumoto, Y., Otsuka, H.
    • Journal Title

      J.Vet. Med. Sci. 63

      Pages: 1139-1143

    • NAID

      110003920762

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takashima, Y., Nagane, N., Hushur, O., Matsu-Moto, Y., Otsuka, H.: "Bovine Herpesvirus-1 (BHV-1) Recombinant Expressing Pseudorabies Virus (PrV) Glycoproteins B and C Induces Type 1 Immune Response in BALB/c mice"J. Vet. Med. Sci. 64. 589-596 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakamichi, K., Matsumoto, Y., Otsuka, H.: "Bovine herpesvirus 1 Us orf8 protein induces apoptosis in infected cells and Facilitates virus egress"Virology. 304. 24-32 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakamichi, K., Matsumoto, Y., Tohya, Y., Otsuka, H.: "Induction of apoptosis in rabbit kidney cell under high-level expression of bovine Herpesvirus 1 Us ORF8 product"Intervirology. 45. 85-93 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakamichi, K., Matsumoto, Y, Otsuka, H: "Defective infection of bovine herpesvirus 1 in non-permissive muline cells."J.Vet.Med.Sci. 63. 1139-1142 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takashima, Y., Nagane, N., Hushur, O., MatsuMoto, Y., Otsuka, H: "Bovine Herpesvirus-1 (BHV-1) Recombinant Expressing Pseudorabies Virus (PrV) Glycoproteins B and C Induces Type 1 Immune Response in BALB/c mice"J.Vet.Med.Sci. (In press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakamichi K.: "Attachment and penetration properties of bovine herpesvirus 1 recombin pseudorabies virus glycoproteins gC and gB"Intervirology. 43. 87-94 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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