| Project/Area Number |
12470011
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Kyushu University |
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Principal Investigator |
KATAFUCHI Toshihiko Kyushu Univ. Dept. of Integrative Physiol. Assistant Professor, 大学院・医学研究院, 講師 (80177401)
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| Co-Investigator(Kenkyū-buntansha) |
TAKE Sachiko Kyushu Univ. Dept. of Integrative Physiol. Assistant Professor, 大学院・医学研究院, 助手 (80253425)
八坂 敏一 九州大学, 大学院・医学研究院, 特別研究員
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2001: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2000: ¥7,200,000 (Direct Cost: ¥7,200,000)
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| Keywords | c-fos mRNA / heat / cold exposure / poly I:C / IFN-α / serotonin transporter / lipopolysaccharide / in vivo microdialysis / stem cell factor / セロトニン再取り込み阻害剤 / 二発刺激増強 / シナプス可塑性 / ストレス / mRNA / IL-1β / COX-2 / bacterial translocation / 環境ストレス / リアルタイムPCR / アンチセンスオリゴDNA / c-fos / 視索前野 / 暑熱暴露 |
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| Research Abstract |
(1) Amount of c-fos mRNA increased in the medial preoptic area (MPO), lateral hypothalamic area (LHA), and ventromedial hypothalamus (VMH) during cold exposure (4 ℃, 1 hr), while CRF mRNA increased in the MPO and paraventricular hypothalamus (PVN) during heat exposure (33 ℃, 1 hr) and in the MPO and LHA during cold. (2) Intraperitoneal injection of poly I:C (mg/kg) in rats induced suppression of spontaneous running wheel activity for more than one week. During this period IFN-α and serotonin transporter (5-HTT) mRNA increased in the cortex and hypothalamus. (3) Heat exposure induced increase in IL-1β, COX-2 and inhibitor of nuclear factor (NF)-κB-β mRNAs, as well as lipopolysaccharide (LPS) content in the cortex of aged rats. Since pretreatment with an endotoxin inhibitor attenuated the increases in these mRNAs and LPS levels, it was suggested that bacterial translocation induced by heat exposure was one of the mechanisms of the increased expression of IL-1β and its related substances mRNAs in the brain. (4) In vivo brain microdialysis revealed that the extracellular concentration of 5-HT in the prefrontal cortex decreased after poly I:C injection. In addition, the poly I:C-induced suppression of the running wheel activity was attenuated by 5-HT1A receptor agonist, 8-hydroxy-2-(di-n-propylamino) tetraline (8-OH-DPAT). (5) Application of a hematopoietic cytokine, stem cell factor (SCF), modulated paired-pulse facilitation in the mossy fiber-CA3 pathway in the mouse hippocampal slices, suggesting that SCF plays a role in the synaptic plasticity in the hippocampus.
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