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Study on molecular structures of synapses and epithelial junctions

Research Project

Project/Area Number 12470023
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionTokyo Medical & Dental University

Principal Investigator

HATA Yutaka  Tokyo Medical & Dental University, Graduate School of Med., Professeor, 大学院・医歯学総合研究科, 教授 (80313237)

Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥12,200,000 (Direct Cost: ¥12,200,000)
Fiscal Year 2001: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 2000: ¥6,800,000 (Direct Cost: ¥6,800,000)
KeywordsSynapse / Epithelial junction / Cell adhesion / Cell polarity / PDZ domain / Catenin / 細胞間結合 / 細胞膜裏打ち蛋白質 / 受容体
Research Abstract

We have investigated the molecular structures of synapses and epithelial junctions. Through this research, we have revealed the following points ;
1. Synaptic scaffolding molecule (S-SCAM) binds p-catenin. This interaction mediates the synaptic targeting of S-SCAM. We have propose the model that b-catenin is first localized at synapses by binding to cadherin, and then recruits to synapses S-SCAM, which provides scaffolds for various components.
2. Brain-enriched guanylate kinase-interacting protein (BEGAIN) is localized in the nucleus as well as at synapses. The synaptic targeting of BEGAIN is mediated by its C-terminal region, possibly by the interaction with PSD-95 and depends on NMDA receptor activity.
3. Membrane-associated guanylate kinase-interacting protein (MAGUIN) is a mammalian homolog of Drosophila Cnk, and binds to PSD-95 and S-SCAM. The synaptic targeting of MAGUIN is mediated by the pleckstrin homology domain and is independent of the interaction with PSD-95 or S-SCAM.
4. Epithelial isoform of S-SCAM named MAGI-1/BAP1 is localized on lateral membranes by its C-terminal PDZ domain.
5. A novel PDZ protein, PAPIN, binds p0071, a member of catenin family. However, the subcellular localization of PAPIN does not depend on the interaction with p0071. PAPIN is localized not only on lateral membranes but also on apical membranes. PAPIN interacts with ERBIN, which binds ErbB2 receptor, via p0071, and may be implicated in the EGF-mediated signaling pathway.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Iida, J. et al.: "Synaptic localization of membrane-associated guanylate kinase-interacting protein mediated by the pleckstrin homology domain"European Journal of Neuroscience. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirabayashi, S. et al.: "C2PA is a nuclear protein implicated in heat shock response"Journal of Cellular Biochemistry. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishimura, W. et al.: "Interaction of synaptic scaffolding molecule and β-catenin"Journal of Neuroscience.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishimura, W. et al.: "Localization of BAI-associated protein1/membrane-associated guanylate kinase-1 at adherens junctions in normal rat kidney cells"Journal of Cellular Physiology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Deguchi, M. et al.: "PAPIN : a novel multiple PSD-95/Dlg-A/ZO-1 protein interacting with neural plakophilin-related armadillo repeat protein/δ-catenin and p0071"Journal of Biological Chemistry.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yao, I. et al.: "Association of membrane-associated guanylate kinase-interacting protein-1 with Raf-1"Biochem. Biophys. Res. Commun..

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Iida, J. et al.: "Synaptic localization of membrane-associated guanylate kinaseinteracting protein mediated by the pleckstrin homology domain"European Journal of Neuroscience. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirabayashi, S. et al.: "C2PA is a nuclear protein implicated in heat shock response"Journal of Cellular Biochemistry. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishimura, W. et al.: "Interaction of synaptic scaffolding molecule and β-catenin"Journal of Neuroscience. 22. 757-765 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishimura, W. et al.: "Localization of BAI-associated protein1/membrane-associated guanylate kinase-1 at adherens junctions in normal rat kidney cells"Journal of Cellular Physiology. 185. 358-365 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Deguchi, M. et al.: "PAPIN : a novel multiple PSD-95/Dig-A/ZO-1 protein interacting with neural plakophilin-related armadillo repeat protein/δ-catenin and p0071"Journal of Biological Chemistry. 275. 29875-29880 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yao, I. et al.: "Association of membrane-associated guanylate kinase-interacting protein-1 with Raf-1"Biochem. Biophys. Res. Commun.. 270. 538-542 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Iida, J. et al.: "Synaptic localization of membrane-associated guanylate kinase-interacting protein mediated by the pleckstrin homology domain"European Journal of Neuroscience. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirabayashi, S. et al.: "C2PA is a nuclear protein implicated in heat shock response"Journal of Cellular Biochemistry. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishimura, W. et al.: "Interaction of synaptic scaffolding molecule and β-catenin"Journal of Neuroscience. 22(3). 757-765 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishimura, W. et al.: "Localization of BAI-associated protein 1/membrane-associated guanylate kinase-1 at adherens junctions in normal rat kidney cells"Journal of Cellular Physiology. 185(3). 358-365 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Deguchi, M. et al.: "PAPIN : a novel multiple PSD-95/Dlg-A/ZO-1 protein interacting with neural plakophilin-related armadillo repeat protein/δ-catenin and p0071"Journal of Biological Chemistry. 275(38). 29875-29880 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yao, I. et al.: "Association of membrane-associated guanylate kinase-interacting protein-1 with Raf-1"Biochem. Biophys. Res. Commun.. 270(2). 538-542 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 西村渉: "Localization of BAP1/MAGI-1 at adherens junctions in NRK cells : the polarized targeting mediated by the carboxyl-terminal PDZ domains."J.Cell. Physiol.. 185巻3号. 358-365 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 出口真紀: "PAPIN : a novel multiple PDZ protein interacting with neural plakophilin-related armadillo-repeat protein/δ-catenin and p0071."J.Biol.Chem.. 275巻38号. 29875-29880 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 平尾和世: "Three isoforms of synaptic scaffolding molecule and their characterization."J.Biol.Chem.. 275巻4号. 2972-2966 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 矢尾育子: "Association of membrane-associated guanylate kinase-interacting protein-1 with Raf-1."Biophys.Biochem.Res.Commun.. 270巻2号. 538-542 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 平尾和世: "Association of synapse-associated protein 90/postsynaptic density-95-associated protein (SAPAP) with neurofilaments."Genes to Cells. 5巻3号. 2966-2972 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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