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Functional analysis of the cells embedded in bone and cartilage matrices

Research Project

Project/Area Number 12470056
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionOsaka University

Principal Investigator

NOMURA Shintaro  Graduate School, Osaka University Medical School, Associate Professor, 医学系研究科, 助教授 (80159087)

Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥12,900,000 (Direct Cost: ¥12,900,000)
Fiscal Year 2001: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2000: ¥10,100,000 (Direct Cost: ¥10,100,000)
Keywordsosteocyte / osteoblast / osteopostin / Bone remodeling / Osteoclast / Bone Matrix / Mechanical stress / Transgenic Mice / メカニカルストレス / プロモーター / GFP / 石灰化
Research Abstract

Head Investigator found that osteocytes, the cell types that embedded in bone matrix, produce osteopontin abundantly when mechanical stress is loaded to bone tissue. Particularly, osteopontin expressing cells located in the pressure side. This result suggest the possibility that osteocyte play important role for bone resorption by producing osteopontin. To test this possibility, chemoattractant activity of osteopontin was determined by chamber experiment. The result obtained clearly demonstrated that osteopontin act as chemoattractant to osteoclast by interaction with integrin α_vβ_5. Furthermore, we tested the bone remodeling activity of osteopontin-deficient mice with the loading of mechanical stress. The absence of bone resorption to the loading of pressure force was observed in osteopontin nockout mice also demonstrate that osteopontin is a key molecule of bone remodeling. In addition, we identified the promoter region which sensitize mechanical stress by producing transgenic mice. Promoter region of osteopontin was joined with reporter gene and injected the DNA fragment into mouse fertilized egg. We detected GFP signal in osteocytes when mechanical stress loaded to bone. The result revealed the function of osteocytes as mechanosensor cells. In addition, we identified the promoter region of osteopontin which required for the expression in hypertrophic chondrocytes. Taken together, knockout mice and transgenic mice generated provide us a new insight for the functional analysis of the cells embedded in bone and cartilage matrix.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Y.Yoshimura. et al.: "Colocalization of Noggin and bone morplugenetic protein during fracture liealing"J. Bone and Mineral. Res. 16. 876-884 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] E.Morii et al.: "Inhibitory effect of MITF eucoded by mutant alleles on GABP-wed : ceted truscript in wouse mast cells"Blood. 97. 3032-3039 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Yasui et al.: "Effects of citrate on renal stone formation and osteopontin expression in a rat urolitlasasis model"Uvol. Res. 29. 151-154 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Himeno et al.: "Impared vasualur in Vakion of Cbfu-1 deficient cartilage auguafted in spleeir"J. Bane and Mimerul Res. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Takakuwa et al.: "Expression of interlaukin-7 and its veceptor in tnyloid lympluner"Lab. Inest. 80. 1483-1490 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y-W.Zhag et al.: "PEBPLαA/cBFA1 mutations in Japanese creidcranial dyspluria patients"Gene. 244. 21-28 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y. Yoshimura et al.: "Colocalization of Noggin and bone morphogenetic protein-4 during fracture healing"J. Bone and Mineral Research. 16. 876-884 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] E. Morii et al.: "Inhibitory effect of MITF encoded by mutant mi allele on GABP-mediated transcript in mouse mast cells"Blood. 97. 3032-3039 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Yasui et al.: "Effects of citrate on renal stone formation and osteopontin expression in a rat urolithiasis model"Urological Res.. 29. 50-56 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Himeno et al.: "Impared vascular invasion of Cbfa1-dificient cartilage engrafted in spleen"J. Bone and Mineral Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Takakuwa et al.: "Expression of interleukin-7 and its receptor in thyroid lymphoma"Lab Invest.. 80. 1483-1490 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y-W.Zhang et al.: "PEBP2αA/CBFA1 mutations in Japanese cleidocrania dysplasia patients"Gene. 244. 21-28 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Yoshimura et al.: "Colocalization of Noggin and bone morphogenetic protein 4 during fracture healing"J.Bone and Mineral Res.. 16. 876-884 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] F.Morii et al.: "Inhibitory effect of MITF encoded by mutant allele on GABP-mediated transcript in mousemart cells"Blood. 97. 3032-3039 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Yasui et al.: "Effect of citrate on rancel stone formation and osteoporosis expression in a rat urobilin air model"Urol. Res.. 29. 151-154 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] H.Himeno, et al.: "Impaired Vascular Invasion of Cbfa-1 Deficient Cartilage Engrafted in the Spleen"J. Bone and Mineral Res.. (印刷中).

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Takakuwa et al.: "Expression of interleukin -7 and its receptor in thyroid lymphouria"Lab. Invest.. 80. 1483-1490 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y-W.-Zhang et al.: "PEBP2αA/CBFA1/ unitations in Japanese creidocrarial dysphasia patients"Gene.. 244. 21-28 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Nomura et al: "Molecular events caused by mechanical stress in bone"Matrix Biol. 19. 91-96 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Fnomoto,S.Nomura et al: "Cbfa-1 is a positive regulatory factor in chondrocyte formation"J.Biol.Chem.. 275. 8695-8702 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.W.Zhang,S.Nomura et al: "PEBP2αA/CBFA1 mutations in Japanese cleidocranial dysplasia patients."Gene. 244. 21-28 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Takauwa,S.Nomura et al.: "Expression of interleukin-7 and its receptor in thyroid lymphemia"Lab.Invest.. 80. 1483-1490 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] S.Nomura et al.: "Molecular Case of the severe functional deficiency in * by an arginine-deletion in the basic domain of * facts."J.Bone and Mines Metal. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Yasui,S.Nomura et al.: "Effects of Allopurinol on Renal Stone Formation and Osteopontin Expression in a Rat Urolithiasis Model"Nephron. 87. 170-176 (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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