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Role of human cytomegalovirus infection and the expression of immediate early gene products (CMV-IE) in the pathogenesis of vascular lesion formations

Research Project

Project/Area Number 12470057
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

YONEMITSU Yoshikazu  Kyushu Univ. Hospital, Assist. Prof., 医学部・附属病院, 助手 (40315065)

Co-Investigator(Kenkyū-buntansha) NAKASIMA Yutaka  Grad. School Med. Sci., Kyushu Univ., Associate Prof., 大学院・医学研究院, 助教授 (50135349)
TSUZUKI Teruhisa  Grad. School Med. Sci., Kyushu Univ., Prof., 大学院・医学研究院, 教授 (40155429)
SUEISHI Katsuo  Grad. School Med. Sci., Kyushu Univ., Prof., 大学院・医学研究院, 教授 (70108710)
NAKAGAWA Kazunori  Grad. School Med. Sci., Kyushu Univ., Senior Assist. Prof., 大学院・医学研究院, 講師 (50217668)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2001: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2000: ¥9,600,000 (Direct Cost: ¥9,600,000)
KeywordsHuman cytomegalovirus / Vasculitis / Angiogenesis / 動脈硬化 / 炎症性腹部大動脈瘤 / 前初期遺伝子
Research Abstract

We previously demonstrated that ;
a) CMV-IE is expressed dominantly and specifically in the aortic walls in patients with inflammatory abdominal aortic aneurysms (IAAA). (Lab Invest 1996a, Nature Med 1998)
b) CMV-IE gene transfer stimulates vascular smooth muscle cell proliferation in vitro and in vivo. (Biochem Biophys Res Common 1997)
c) Vascular endothelial growth factor (VEGF) is abundantly expressed in the aortic wall of IAAA, and VEGF gene transfer to rabbit carotid arteries stimulated not only neointimal formation, but also "leaky, and angioma-like" fragile vessels in the neointima. (Lab Invest 1996b), however, there has been no study assessing the exact roles of CMV-IE in the inflammatory and angiogenic events in vessel wall.
In the present study, we 1) tried to establish mouse lines expressing CMV-IE1 or CMV-IE2 specifically in vessel wall, and 2) assessed the molecular mechanisms of formation of "fragile" (alternatively, non-functional ) angiogenesis using recombinant viral vecto … More rs.
1) Establishment of CMV-IE transgenic mice (TG) :
To perform vessel specific expression of CMV-IE, we used murine preproendothelin-1 promoter. We obtained 2 lines of CMV-IE1 TG and 5 lines of CMV-IE2 in F1 generation, and gene expression assessments are now under evaluation. At this time, no apparent phenotype was found in each F1 animal. Establishment of F2 and generation of TG co-expressing CMV-IE1 and -IE2 are now assessed.
2) Molecular mechanisms of formation of "fragile" angiogenesis :
We tested effects of overexpression of VEGF165 using recombinant Sendai virus (SeV), as directly compared to FGF2 gene transfer. Intramuscular injection of SeV strongly boosted FGF2, resulting in significant therapeutic effects for limb salvage with increased blood perfusion associated with enhanced endogenous VEGF expression in murine models of critical limb ischemia. Incontrast, VEGF165-overexpression, 5-times higher than that of baseline on day 1, also strongly evoked endogenous VEGF in muscles, resulting in an accelerated limb amputation without recovery of blood perfusion. Interestingly, viable skeletal muscles of either VEGF165- or FGF2-treated ischemic limbs showed similar PCAM-1-positive vessel densities. Maturation of newly formed vessels suggested by smooth muscle cell actin-positive cell lining, however, was significantly disturbed in muscles with VEGF. Further, therapeutic effects of FGF2 were completely diminished by anti-VEGF neutralizing antibody in vivo, thus indicationg that endogenous VEGF does contribute to the effect of FGF2. These results suggest that VEGF is necessary, but should be delicately regulated to lower expression to treat ischemic limb. The therapeutic effect of FGF2, associated with the harmonized angiogenic effects seen with endogenous VEGF, provides important insights into therapeutic angiogenesis. Less

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (92 results)

All Other

All Publications (92 results)

  • [Publications] Masaki I, et al.: "Recombinant Sendai Virus-mediated Gene Transfer to Vasculature : a new class of efficient gene transfer vector to the vascular system"The FASEB Journal. 15. 1294-1296 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hasegawa H, et al.: "Preclinical and therapeutic utility of HVJ-liposomes as a gene transfer vector for hepatocellular carcinoma using herpes simplex thymidine kinase"Cancer Gene Therapy. 8. 252-258 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Teramoto N, et al.: "Dual action of ZD6169, a novel K+channel opener. on ATP-sensitive K+channels in pig urethral myocytes"British Journal of Pharmacology. 133. 154-164 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Komori K, et al.: "Non-penetrating vascular clips anasromosis inhibited intimal thickening under poor runoff conditions in canine autogenous vein grafts"European Journal of Vascular and Endovascular Surgery. 21. 241-247 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koga T, et al.: "Heterogenous distribution of p53 immunoreactivity in human lung adenocarcinoma correlates with MDM2 protein expression. rather than with p53 gene mutation"International Journal of Cancer. 95. 232-239 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koga T, et al.: "Clinico-pathological and molecular evidence indicationg the independency of bronchio-alveolar components from other subtypes of human lung"Clinical Cancer Research. 7. 1730-1738 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yonemitsu Y, et al.: "Efficient gene transfer to airway epithelium using recombinant Sendai virus"Nature Biotechnology. 18. 970-973 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakagawa K, et al.: "Angiogenesis and its regulation : roles of vascular endothelial cell growth factor"Seminars in Thrombosis and Hemostasis. 26. 61-66 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sugimachi K, Yonemitsu Y, et al.: "A conservative treatment for the removal of a central venous catheter with large thrombus : a case report"Nutrition in Clinical Ptactice. 15. 223-226 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Teramoto N, et al.: "The effects pf caffeine on ATP-sensitive K+currents in smooth muscle cells from pig urethra"British Journal of Pharmacology. 131. 515-523 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takeushi K, et al.: "A simultaneous resection of a concomitant abdominal aortic aneurysm and hepatocellular carcinoma : two cases"Int Surg. 85. 152-157 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ishida M, et al.: "Immunohistochemical phenotypic alterations of rabbitt autologous vein grafts implanted under arterial circulation with or without poor distal runoff-implications of vein graft remodeling"Atherosclerosis. 154. 345-354 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsumoto T, et al.: "Successful and optimized in vivo gene transfer to rabbit carotid artery mediated by electronic pulse"Gene Therapy. 8. 1174-1179 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakamoto T, et al.: "Gene targeting to the retina"Advanced Drug Delivery Reviews. 52. 93-102 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamashita A, Yonemitsu Y, et al.: "Fibroblast growth factor-2 determines severity of joint disease in adjuvant induced arthritis in rats"The Journal of Immunology. 168. 450-457 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yonemitsu Y, et al.: "Gene Therapy in Vascular Surgery Comes of Age"Surgery. 131. S261-S268 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kume M, et al.: "Administration of a decoy against the AR-1 binding site suppresses neointimal thickening in rabbit balloon injured arteries"Circulation. 105. 1226-1232 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikeda Y, Yonemitsu Y, et al.: "Recombinant Sendai virus-mediated gene transfer to the retinal tissue of adult rats : efficient gene transfer by brief exposure"Experimental Eye Research. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koga T, et al.: "Lung adenocarcinoma with bronchio-alveolar carcinoma frequently associates foci of high grade atypical adenomatous hyperplasia"American Journal of Clinical Pathology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 真崎一郎, 長谷川護, 米満吉和: "Trend Paper:組換えセンダイウイルスベクターによる遣伝子治療:純国産ペクターは遺伝子治療に新しい道を開くか?"バイオ・ベンチャー. 1. 85-88 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 米満吉和, 居石克夫: "わだい:サイトメガロウイルスと動脈硬化"Medical Technology. 29. 386-388 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 米満吉和, 他: "重症虚血肢に対する血管新生因子VEGF、FGF2遺伝子導入の効果とその意義"厚生省特定疾患難治性血管炎に関する調査研究班(橋本班)平成12年度総括研究報告書. 79-82 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 居石克夫, 米満吉和, 他: "血管壁細胞の遺伝子発現制御と治療への応用"脈管学. 41. 501-509 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 米満吉和, 居石克夫: "ウイルス感染と大型血管炎"脈管学. 41. 381-390 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 坂本泰三, 池田康博, 米満吉和: "網膜への遺伝子ターゲティング(Gene Targeting to Retina)"あたらしい眼科. 18. 41-46 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 庄司文裕, 長谷川 護, 米満吉和: "組換えセンダイウイルスベクターを用いた血管壁への遺伝子導入"脳と循環. 7. 53-56 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 米満吉和, 居石克夫: "特集「大動脈炎症候群をどう診るか亅10.ウイルス感染と大型血管炎"Heart View. 6. 88-93 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 米満吉和, 他: "血管新生遺伝子治療における「血管新生因子作用機構の階層性亅と「"integrated" Therapeutic Angiogenesis」の提唱"脈管学. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 米満吉和, 他(分担執箪): "『血管内科』 第2章血管生物学の基礎:血管壁の構成細胞"メディカルレビュー社. 678 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koga T, Hashimoto S, Yonemitsu Y, Sugio K, Matsuo Y, Mojtahedzadeh S, Yoshino I, Fujii H, Sugimachi K, Sueishi K.: "Lung adenocarcinoma with bronchio-alveolar carcinoma component frequently associates foci of high grade atypical adenomatous hyperplasia"American Journal of Clinical Pathology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikeda Y, Yonemitsu Y, Ishibashi T, Murata T, Ueno H, Kato A, Nagai Y, Fukumura M, Inomata H, Hasegawa M, Sueishi K.: "Recombinant Sendai virus-mediated gene transfer to the retinal tissue of adult rats : efficient gene transfer by brief exposure"Experimental Eye Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yonemitsu Y, Komori K, Sueishi K, Sugimachi K.: "Gene Therapy in Vascular Surgery Comes of Age"Surgery. 131. S261-S268 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kume M, Komori K, Matsumoto T, Onohara T, Takeuchi K, Yonemitsu Y, Sugimachi K.: "Administration of a decoy against the AP-1 binding site suppresses neointimal thickening in rabbit balloon injured arteries"Circulation. 105. 1226-1232 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamashita A, Yonemitsu Y, Okano S, Nakagawa K, Nakashima Y, Iwamoto Y, Nagai Y, Hasegawa M, Sueishi K.: "Fibroblast growth factor-2 determines severity of joint disease in adjuvant-induced arthritis in rats"The Journal of Immunology. 168. 450-457 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakamoto T, Ikeda Y. Yonemitsu Y.: "Gene targeting to the retina"Advanced Drug Delivery Reviews. 52. 93-102 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsumoto T, Komori K, Shouji T, Kuma S, Kume M, Yamaoka T, Mori E, Furuyama T, Yonemitsu Y, Sugimachi K.: "Successful and optimized in vivo gene transfer to rabbit carotid artery mediated by the electronic pulse"Gene Therapy. 8. 1174-1179 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koga T, Hashimoto S, Sugio K, Yoshino I, Mojtahedzadeh S, Matsuo Y, Yonemitsu Y, Sugimachi K, Sueishi K.: "Clinico-pathological and molecular evidence indicating the independency of bronchio-alveolar components from other subtypes of human lung adenocarcinoma"Clinical Cancer Research. 7. 1730-1738 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koga T, Hashimoto S, Sugio K, Yoshino I, Nakagawa K, Yonemitsu Y, Sugimachi K, Sueishi K.: "Heterogenous distribution of p53 immunoreactivity in human lung adenocarcinoma correlates with MDM2 protein expression, rather than with p53 gene mutation"International Journal of Cancer. 95. 232-239 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakamoto T, Ito S, Yoshikawa H, Hata Y, Ishibashi T, Sueishi K, Inomata H.: "Tissue factor increases in the aqueous humor of proliferative diabetic retinopathy"Graefe's Archives of Clinical and Experimental Ophthalmology. 239. 865-871 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hasegawa H, Shimada M, Yonemitsu Y, Utsunomiya T, Gion T, Kaneda Y, Sugimachi K.: "Preclinical and therapeutic utility of HVJ-liposomes as a gene transfer vector for hepatocellular carcinoma using herpes simplex thymidine kinase"Cancer Gene Therapy. 8. 252-258 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Komori K, Shoji T, Furuyama T, Yonemitsu Y, Mori E, Yamaoka T, Matsumoto T, Sugimachi K.: "Non-penetrating vascular clips anastomosis inhibited intimal thickening under poor runoff conditions in canine autogenous vein grafts"European Journal of Vascular and Endovascular Surgery. 21. 241-247 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Teramoto N, Yunoki T, Takano M, Yonemitsu Y, Masaki I, Sueishi K, Brading AF, Ito Y.: "Dual action of ZD6169, a novel K+ channel opener, on ATP-sensitive K+ channels in pig urethral myocytes"British Journal of Pharmacology. 133. 154-164 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masaki I, Yonemitsu Y, Komori K, Ueno H, Nakashima Y, Nakaoawa K, Fukumura M, Kato A, Hasan MK, Nagai Y, Sugimachi K, Hasegawa M, Sueishi K.: "Recombinant Sendai Virus-mediated Gene Transfer to Vasculature : a new class of efficient gene transfer vector to the vascular system"The FASEB Journal 10.1096/fj.00-0460fje (online version), 2001. The FASEB Journal. 15 (print version). 1294-1296 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ishibashi H, Shiratuchi T, Nakagawa K, Onimaru M, Sugimachi T, Sueishi K, Shirasuna K.: "Hypoxia-induced angiogenesis of cultured human salivary gland carcinoma cells enhances vascular endothelial growth factor production and basie fibroblast growth factor release"Oral Oncology. 37. 77-83 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Imakita M, Yutani C, Strong JP, Sakurai I, Sumiyoshi A, Watanabe T, Mitsumata M, Kusumi Y, Katayama S, Mano M, Baba S, Mannami T, Masuda J, Sueishi K, Tanaka K.: "Second nation-wide study of atherosclerosis in infants, children and young adults in Japan"Atherosclerosis. 155. 487-497 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shimizu I, Tomita Y, Iwai T, Zhang QW, Nakashima Y, Sueishi K, Yasui H.: "Heart allogeraft tolerance without developement of posttransplant cardiac allograft vasculopathy in cyclophosphamide-induced tolerance"Transplant Proc.. 33. 121-126 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ishida M, Komori K, Yonemitsu Y, Matsumoto T, Onohara T, Sugimachi K.: "Immunohistochemical phenotypic alterations of rabbitt autologous vein grafts implanted under arterial circulation with or without poor distal runoff -implications of vein graft remodeling"Atherosclerosis. 154. 345-354 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Teramoto N, Yunoki T, Tanaka K, Takano M, Masaki I, Yonemitsu Y, Sueishi K, Ito Y.: "The effects of caffein on ATP-sensitive K+currents in smooth muscle cells form pig urethra"Br J Pharmacol. 131. 515-523 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kitamoto S, Egashira K, Kataoka C, Koyanagi M, Katoh M, Shimokawa H, Morishita R, Kaneda Y, Sueishi K, Takeshita A.: "increased activity of nuclear factor-kappaB participates in cardiovascular remodeling induced by chronici inhibition of nitric oxide synthesis in rats"Circulation. 102. 806-812 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takeuchi K, Komori K, Ohta S, Shimada M, Yonemitsu Y, Sugimachi K.: "A simultaneous resection of a concomitant abdominal"International Surgery. 85. 152-157 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshikawa H, Ishibashi T, Hata Y, Inomata H, Sueishi K.: "The Suppressive effect of tecogalan sodium on in vitro angiogenesis via the periendothelial preteolytic activities"Ophthalmic Research. 32. 261-269

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Egashira K, Koyanagi M, Kitamoto S, Ni W, Kataoka C, Morishita R, Kaneda Y, Akiyama C, Nisida K, Sueishi K, Takeshita A.: "Anti-monocyte chemoattractant protein-1 gene therapy inhibits vascular remodeling in rats : blockade of MCP-1 activity after intramuscular transfer of a mutant gene inihibitis vascular remodeling induced by chroni blockade of NO synthesis"The FASEB Journal. 14. 1974-1978 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ishibashi H, Nakagawa K, Onimaru M, Castellanous EJ, Chen YX, Shirasuna K, Sueishi K.: "Novel anti-angio-genic gene therapy by cis-trans element decoy system against TNF-a induced Sp-1 transactivation"Cancer Research. 60. 6531-6536 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakagawa K, Chen Y-X, Ishibashi H, Yonemitsu Y, Murata T, Hata Y, Nakashima Y, Sueishi K.: "Angiogenesis and its regulation : roles of vascular endothelial cell growth factor"Seminars in Thrombosis and Hemostasis. 26. 61-66 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Teramoto N, Yunoki T, Tanaka K, Takano M, Masaki I, Yonemitsu Y, Sueishi K, Ito, Y.: "The effects of caffeine on ATP-sensitive K+ currents in smooth muscle cells from pig urethra"The British Journal of Pharmacology. 131. 515-523 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yonemitsu Y, Kitson C. Ferrari S, Farley R. Griesenbach U, Dian J, Steel R, Phillipe S, Zhu J, Jeffery PK, Kato A, Hasan MK, Masaki I, Nagai Y, Fukumura M, Hasegawa M, Geddes DM, Alton EWFW.: "Efficient gene transfer to the airway epithelium using recombinant Sendai virus"Nature Biotechnology. 18. 970-973 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sugimachi Keishi, Yonemitsu Y, Komori K, Sugimachi Keizo.: "A conservative treatment for the removal of a central venous catheter with large thrombus : a case report"Nutrition in Clinical Practice. 15. 223-226 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yonemitsu Y, et al.: "Efficient gene transfer to airway epithelium using recombinant Sendai virus"Nature Biotechnology. 18. 970-973 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakagawa K, et al.: "Angiogenesis and its regulation ; roles of vascular endothelial cell growth factor"Seminars in Thrombosis and Hemostasis. 26. 61-66 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sugimachi K, Yonemitsu Y et al.: "A conservative treatment for the removal of a central venous catheter with large thrombus : a case report"Nutrition in Clinical Ptactice. 15. 223-226 (2000)

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      2001 Annual Research Report
  • [Publications] Teramoto N, et al.: "The effects pf caffeine on ATP-sensitive K+currents in smooth muscle cells from pig urethra"British Journal of Pharmacology. 131. 515-523 (2000)

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      2001 Annual Research Report
  • [Publications] Takeushi K, et al.: "A simultaneous resection of a concomitant abdominal aortic aneurysm and hepatocellular carcinoma : two cases"Int Surg. 85. 152-157 (2000)

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      2001 Annual Research Report
  • [Publications] Ishida M, et al.: "Immunohistochemical phenotypic alterations of rabbitt autologous vein grafts implanted under arterial circulation with or without poor distal runoff-implications of vein graft remodeling"Atherosclerosis. 154. 345-354 (2001)

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      2001 Annual Research Report
  • [Publications] Masaki I, et al.: "Recombinant Sendai Virus-mediated Gene Transfer to Vasculature : a new class of efficient gene transfer vector to the vascular system"The FASEB Journal. 15. 1294-1296 (2001)

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      2001 Annual Research Report
  • [Publications] Hasegawa H, et al.: "Preclinical and therapeutic utility of HVJ-liposomes as a gene transfer vector for hepatocellular carcinoma using herpes simplex thymidine kinase"Cancer Gene Therapy. 8. 252-258 (2001)

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      2001 Annual Research Report
  • [Publications] Teramoto N, et al.: "Dual action of ZD6169, a novel K+channel opener, on ATP-sensitive K+channels in pig urethral myocytes"British Journal of Pharmacology. 133. 154-164 (2001)

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      2001 Annual Research Report
  • [Publications] Komori K, et al.: "Non-penetrating vascular clips anasromosis inhibited intimal thickening under poor runoff conditions in canine autogenous vein grafts"European Journal of Vascular and Endovascular Surgery. 21. 241-247 (2001)

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      2001 Annual Research Report
  • [Publications] Koga T, et al.: "Heterogenous distribution of p53 immunoreactivity in human lung adenocarcinoma correlates with MDM2 protein expression, rather than with p53 gene mutation"International Journal of Cancer. 95. 232-239 (2001)

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      2001 Annual Research Report
  • [Publications] Koga T, et al.: "Clinico-pathological and molecular evidence indicationg the independency of bronchio-alveolar components from other subtypes of human lung"Clinical Cancer Research. 7. 1730-1738 (2001)

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  • [Publications] Matsumoto T, et al.: "Successful and optimized in vivo gene transfer to rabbit carotid artery mediated by electronic pulse"Gene Therapy. 8. 1174-1179 (2001)

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      2001 Annual Research Report
  • [Publications] Sakamoto T. er al.: "Gene targeting to the retina"Advanced Drug Delivery Reviews. 52. 93-102 (2001)

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  • [Publications] Yamashita A, Tonemitsu Y, et al.: "Fibroblast growth factor-2 determines severity of joint disease in adjuvant induced arthritis in rats"The Journal of Immunology. 168. 450-457 (2002)

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  • [Publications] Yonemitsu Y, et al.: "Gene Therapy in Vascular Surgery Comes of Age"Surgery. 131. S261-S268 (2002)

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  • [Publications] Kume M, et al.: "Administration of a decoy against the AP-1 binding site suppresses neointimal thickening in rabbit balloon injured arteries"Circulation. 105. 1226-1232 (2002)

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  • [Publications] Ikeda Y, Yonemitsu Y, et al.: "Recombinant Sendai virus-mediated gene transfer to the retinal tissue of adult rats : efficient gene transfer by brief exposure"Experimental Eye Research. (in press). (2002)

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  • [Publications] Koga T, et al.: "Lung adenocarcinoma with bronchio-alveolar carcinoma frequently associates foci of high grade atypical adenomatous hyperplasia"American Journal of Clinical Pathology. (in press).

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  • [Publications] 真崎一郎, 長谷川護, 米満吉和: "Trend Paper :組換えセンダイウイルスベクターによる遺伝子治療:純国産ベクターは遺伝子治療に新しい道を開くか?"バイオ・ベンチャー. 1. 85-88 (2001)

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  • [Publications] 米満吉和, 居石克夫: "わだい:サイトメガロウイルスと動脈硬化"Medical Technology. 29. 386-388 (2001)

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  • [Publications] 米満吉和 他: "重症虚血肢に対する血管新生因子VEGF、FGF2遺伝子導入の効果とその意義"厚生省特定疾患難治性血管炎に関する調査研究班(橋本班) 平成12年度総括研究報告書. 79-82 (2001)

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  • [Publications] 居石克夫, 米満吉和 他: "血管壁細胞の遺伝子発現制御と治療への応用"脈管学. 41. 501-509 (2001)

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  • [Publications] 米満吉和, 居石克夫: "ウイルス感染と大型血管炎"脈管学. 41. 381-390 (2001)

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  • [Publications] 坂本泰二, 池田康博, 米満吉和: "網膜への遺伝子ターゲティング(Gene Targeting to Retina)"あたらしい眼科. 18. 41-46 (2001)

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  • [Publications] 庄司文裕, 長谷川 護, 米満吉和: "組換えセンダイウイルスベクターを用いた血管壁への遺伝子導入"脳と循環. 7. 53-56 (2002)

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  • [Publications] 米満吉和, 居石克夫: "特集「大動脈炎症候群をどう診るか」 10. ウイルス感染と大型血管炎"Heart View. 6. 88-93 (2002)

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  • [Publications] 米満吉和 他: "血管新生遺伝子治療における「血管新生因子作用機構の階層性」と「"Integrated" Therapeutic Angiogenesis」の提唱"脈管学. (印刷中).

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  • [Publications] 米満吉和 他(分担執筆): "『血管内科』 第2章血管生物学の基礎:血管壁の構成細胞"メディカルレビュー社. 678 (2001)

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  • [Publications] Masaki I,Yonemitsu Y, et al.: "Recombinant Sendai Virus-mediated Gene Transfer to Vasculature : a new class of efficient gene transfer vector to the vascular system."The FASEB Journal. 15(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hasegawa H, et al.: "Preclinical and therapeutic utility of HVJ-liposomes as a gene transfer vector for hepatocellular carcinoma using herpes simplex thymidine kinase"Cancer Gene Therapy. 8(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Teramoto N, et al.: "Dual action of ZD6169, a novel K+ channel opener, on ATP-sensitive K+ channels in pig urethral myocytes."The British Journal of Pharmacology. 132(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ishida M, et al.: "Immunohistochemical phenotypic afterations of rabbit autologous vein grafts implanted under arterial circulation with or without poor distal runoff : implications of vein graft remodeling."Atherosclerosis. 154. 345-354 (2001)

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      2000 Annual Research Report
  • [Publications] Yonemitsu Y, et al.: "Efficient gene transfer to the airway epithelium using recombinant"Nature Biotechnology. 18. 970-973 (2000)

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      2000 Annual Research Report
  • [Publications] Teramoto N, et al.: "The effect of caffeine on ATP-sensitive K+ currents in smooth muscle cells from pig urethra."The British Journal of Pharmacology. 131. 515-523 (2000)

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      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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