| Project/Area Number |
12470060
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | Mie University |
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Principal Investigator |
YUDA Masao Mie University, Faculty of Medicine, Research Associate, 医学部, 助手 (90293779)
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| Co-Investigator(Kenkyū-buntansha) |
ANDO Kastuhiko Mie University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (90024710)
CHINZEI Yasuo Mie University, Faculty of Medicine, Professor, 医学部, 教授 (60024709)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥14,300,000 (Direct Cost: ¥14,300,000)
Fiscal Year 2002: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2001: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2000: ¥8,800,000 (Direct Cost: ¥8,800,000)
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| Keywords | Malaria / mosquite / ookinete / sporozoite / infection / molecular mechanisms / ネズミマラリア / CTRP / ノックアウト原虫 / ハマダラカ / 接着侵入 |
|---|
| Research Abstract |
To search for genes playing roles in malarial infection of the mosquito vector, we constructed EST databases of the vector stages of rodent malarial parasite. We selected some genes from these databases and prepared knock-out parasites of each gene. As criteria for selection, we adopted presence of the N-terminal signal peptide, because this indicates that the gene products may play a role in parasite interactions with the vector mosquito. In this study, we identified some genes that play a critical role in malarial parasite infection of mosquito organs. One of them encodes 21kDa protein with a secretory protein-lilke structure and is necessay for ookinete invasion into the midgut epithelium. We performed immuno-electeon microscopy and found that this protein is localized in the micronemes. Other one encodes an attachment protein-like molecule and is expressed by the sporozoite in the oocyst but not by the salivary gland sporozoite. By disruption of this gene, we demonstrated that it is essential for the malarial parasite to infect the mosquito salivary gland.
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