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Study to prepare safe educational bacterial strains for medical education

Research Project

Project/Area Number 12470062
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionGifu University

Principal Investigator

EZAKI Takayuki  Gifu Univ., Microbiology, Professor, 医学部, 教授 (90151977)

Co-Investigator(Kenkyū-buntansha) KURAZONO Hisaso  Okayama Univ., Microbiology, Professor, 医学部, 教授 (90186487)
LIDA Tetsuya  Osaka Univ., Microbiology, Assist. Professor, 微生物病研究所, 助教授 (90221746)
MAKINO Souichi  Obihiro Chikusan Univ., Microbiology, Assist. Professor, 畜産学部, 助教授 (30181621)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥12,300,000 (Direct Cost: ¥12,300,000)
Fiscal Year 2001: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 2000: ¥6,900,000 (Direct Cost: ¥6,900,000)
KeywordsLebel 2 / Lebel 3 / Education / Biosafty / Pathogen / Medicine / 感染症 / 病原細菌 / 危険度レベル / 教育用菌株
Research Abstract

Through this study, we constructed safe bacterial strains for educational purpose. Because level 3 and potent toxigenic level 2 bacterial pathogens are requested to use for medical education. However, most of university does not have appropriate facility for education. Class 3 facility is not prepared for student teaching room at most of medical universities. We collected information to decrease the level of pathogenicity for level 3 pathogens and selected candidate strains for educational purpose.
Most candidate strains are knocked out their pathogenic factors. We propose to decrease biosafty level of these knocked out strains ofpathogenic factors. These strains should be widely distributed among researchers to evaluate their safety level in future

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Zhao, L., et al.: "Construction of virulent defective mutants …"Microbiol. Cult. Coll. 17. 13-21 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Zhao, L., et al.: "Vi-suppressed wild stoain Salmonella typhi …"Microbiol. Immunol. 45. 149-158 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Iida, T., et al.: "Filamentous phage hogociated with recent …"Emerg. Infect. Dis.. 7. 477-478 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takahashi, A., et al.: "Chloride secretion induced by thermostoble …"J. Med. Microbiol. 50. 870-878 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakano, M., et al.: "Structural and sequence diversity of the"FEMS Microbiol. Lett. 205. 71-76 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamamoto, S., et al.: "The presence of virulence island containing"J. Urol. 165. 1347-1351 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Zhao,L.1, Ezaki,T., Li,Z.Y., Kawamura,Y., Hirose,K., Watanabe: "Vi-suppressed wild strain Salmonella typhi cultured in high osmolarity is hyperinvasive to epithelial cells and destructive of Peyer's patches"Microbio. Immunol. 45. 149-158 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Licheng,Zhao, Kawamura,Yoshiaki and Ezaki,Takayuki: "Construction of virulent defective mutants of Salmonella typhi and their phenotypic description as candidates for educational purposes"Microbio.Cult. Coll. 17. 13-21 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] lida,T., Hattori,A., Tagomori,K., Nasu,H., Naim,R, Honda,T.: "Filamentous phage associated with recent pandemic strains of Vibrio parahaemolyticus"Emerg Infect Dis.. 7 ( 3 ). 477-478 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takahashi,A., lida,T., Naim,R, Naykaya,Y., Honda,T.: "Chloride secretion induced by thermostable direct haemolysin of Vibrio parahaemolyticus depends on colonic cell maturation"J Med Microbiol. 50 ( 10 ). 870-878 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakano,M., Yamamoto,S., Terai,A., Ogawa,O., Makino,SI., Hayashi,H., Nair,GB., Kurazono,H.: "Structural and sequence diversity of the pathogenicity island of uropathogenic Escherichia coli which encodes the USP protein"FEMS Microbiol Lett. 205 ( 1 ). 71-76 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamamoto,S., Nakano,M., Terai,K., Nakata,K., Nair GB., Kurazono,H., Ogawa,O.: "The presence of the virulence island contains usp gene in uropathogenic Escherichia coli is associated with tract infection in an experimental mouse"J. Urol. 165. 1347-1351 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 江崎 孝行: "Vi-suppressed wild strain Salmonella typhi cultured in high osmolarity is hyperinvasive to epithelial cells and destructive of Peyers patches"Microbiol. Immunol.. 45. 149-158 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 江崎 孝行: "Construction of virulent defective mutants of Salmonella typhi and their phenotypic description as candidates for educational purposes"Microbiol. Calt. Cell.. 17. 13-21 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 江崎 孝行: "Selective amplification of tyr(rfbE), prt(rfbs), riaB and fliC genes by murtiplex PCR for identification of Salmonella enterica serorar typhi, and paratyphi A"J. Clin. Microbiol.. 140. (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] "DNAマイクロアレイを用いた細菌の検出および同定"臨床検査. 45. 776-778 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 江崎 孝行: "医学細菌の分類命名の情報"感染症学雑誌. 75. 833-836 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 江崎 孝行: "ゲノム解析と臨床微生物への応用"臨床と微生物. 28. 735-743 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Licheng Zhao: "Vi Suppressed Wild Strain Salmonella Typhi Cultured"Microbiology Immunol. 45・2. 149-158 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Licheng Zhao: "Construction of Virulent defective mutants of …"日本微生物資原学会誌. (In.Press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 江崎孝行: "病原体の取り抜い及び同定のための手引き"病原細菌研究会. 129 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 江崎孝行: "日本の微生物分類-この20年"微生物分類研究会. 158 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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