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A study on the function of cell-cycle specific inhibitor, CDT

Research Project

Project/Area Number 12470064
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

SUGAI Motoyuki  Faculty of Dentistry, HIROSHIMA UNIVERSITY, Professor, 歯学部, 教授 (10201568)

Co-Investigator(Kenkyū-buntansha) OHARA Masaru  Faculty of Dentistry, HIROSHIMA UNIVERSITY, Research Assistant, 歯学部, 助手 (80253095)
FUJIWARA Tamaki  Faculty of Dentistry, HIROSHIMA UNIVERSITY, Research Assistant, 歯学部, 助手 (90274092)
KOMATSUZAWA Hitoshi  Faculty of Dentistry, HIROSHIMA UNIVERSITY, Associate Professor, 歯学部, 助教授 (90253088)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥14,300,000 (Direct Cost: ¥14,300,000)
Fiscal Year 2001: ¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 2000: ¥7,600,000 (Direct Cost: ¥7,600,000)
KeywordsCytolethal distending toxin / ctinibacillus actinomycetemcomitams / CDTABC complex / lipoptrotein / apoptosis / 細胞膨化致死毒素 / G2 / Mブロック / Actinobacillus actinomycetemcomitans / CDT / cell cycle / 毒素 / 歯周病 / 細胞周期
Research Abstract

CDT from Actinobacillus actinomycetemcomitans (A.a) is composed of CDTA, B, and C encoded as cdtA, cdtB and cdtC genes tandemly-located on the chromosomal cdt locus. Immunoprecipitation study indicates that CDT forms complex. N-terminal sequencing of purified CDT revealed that 36-43 amino acids of CDTA are further processed at N-terminus probably after lipidmodification of CDTA. Sucrose density gradient ultracentrifuge showed the localization of CDT complex at the outer membrane. Taken together, we propose the hypothetical pathway of CDT secretion from periplasmic space to culture supernatant.
CDT-induced death in T cell lines was accompanied with the biochemical features of apoptosis including membrane conformational change, intranucleosomal DNA cleavage, and increase of caspase activity in the cells. Inhibitors for caspase-2 and -7 showed significant inhibitory effect on CDT-induced apoptosis of Jurkat cells suggesting that CDT possesses an ability to induce human T cell apoptosis through activation of caspase-2 and -7.
Among 45 A.a clinical isolates, CDT activity was found in cell lysate and culture supernatant of all tested strains, but the titer of the toxin in culture supernatant considerably varied among strains. PCR experiments indicated the presence of Y4-type cdt sequences in forty strains, suggesting that CDT production is prevalent in A.a strains.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Ryousuke Yamano: "Prevalence of cytolethal distending toxin production in periodontopathogenic bacteria"J. Clin. Microbiol. 41(In press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 小原勝: "細菌毒素ハンドブック"桜井純, 本田武司, 小熊恵二. 7 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ryousuke Yamano et al.: "Prevalence of cytolethal distending toxin production in period on topathogenic bacteria"J. Clin. Microbiol. Vol.41(In press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masaru Ohara et al.: "Handbook on bacterial toxin Jun Sakurai, Takeshi Honda, Keiji Koguma eds"23-29 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary

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Published: 2000-04-01   Modified: 2016-04-21  

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