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Regulation of paramyxovirus gene expression and pathogenesis by host factors.

Research Project

Project/Area Number 12470071
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionHiroshima University

Principal Investigator

YOSHIDA Tetsuya  Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (00022905)

Co-Investigator(Kenkyū-buntansha) KIYOTANI Katsuhiro  Hiroshima University, Graduate School of Biomedical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 講師 (00106824)
SAKAGUCHI Takemasa  Hiroshima University, Graduate School of Biomedical Sciences, Associate Professor, 大学院・医歯薬学総合研究科, 助教授 (70196070)
Project Period (FY) 2000 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2001: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywordsparamyxovirus / Sendai virus / pathogenicity / gene expression / V protein / innate immunity / leader sequence / host factors / 弱毒性 / リバースジェネティクス / 浜松株 / 弱毒化 / リーダー変異 / ウイルス回収系 / V蛋白 / ゲノム複製 / 塩基配列 / ウイルス回收系 / Zn結合能
Research Abstract

Sendai virus (SeV) is a paramyxovirus causing bronchopneumonitis in rodents and possesses a nonsegmented single-stranded minus-sense RNA as a genome. In the present study we examined the function of SeV accessory V protein and the attenuation of SeV pathogenicity by egg passages to study the regulation of SeV gene expression and pathogenesis by host factors, and the following results were obtained.
(1) The V protein of the Hamamatsu strain, a highly virulent field SeV isolate, has been shown to have a luxury function to facilitate virus propagation in mice but not in cultured cells as well as that of the Z strain, an egg-adapted avirulent laboratory SeV strain, and to be prominent only if virus replication was restricted. (ref. 4, 8, 14, and 17)
(2) The luxuary function of the V protein has been shown to be dependent on the amino acid residues at the C terminus of the protein, which are highly conserved among paramyxoviruses, probably via protein conformation dependent on Zn binding. (ref. 1, 4, 8, and 13)
(3) The V protein function, probably by coping with the host innate immunity, antagonized neither interferon action nor NK cell activity. The cellular target of the V protein remains to be clarified. (ref. 17, unpublished data)
(4) Attenuation of SeV pathogenicity by egg passages has been shown to be caused at least by mutations of the nucleotides at position 20 and 24 in the leader sequence, suggesting that the leader mutaions affect virus pathogenesis by altering virus replication via interaction with host factors. (ref. 6, 12, and 14)

Report

(5 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (68 results)

All Other

All Publications (68 results)

  • [Publications] Huang, C.: "Involvement of the zinc-binding capacity of Sendai virus V protein in viral pathogenesis."Journal of Virology. 74. 7834-7841 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Eguchi, A.: "Identification and characterization of cell lines with. a defect in a post-adsorption stage of Sendai virus-mediated membrane fusion."Journal of Biological Chemistry. 275. 17549-17555 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 坂口剛正: "抗ウイルス薬を中心とした治療"呼吸と循環. 48. 1127-1131 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujii, Y.: "Conserved and non-conserved regions in the Sendai virus genome : evolution of a gene possessing overlapping reading frames."Virus Genes. 22. 47-52 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fan, X.-H.: "Emergence of anti-inflammatory monocytes in long-term surviving host of IL-10-transduced liver allografts."Cytokine. 13. 183-187 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kiyotani, K.: "Attenuation of a field Sendai virus isolate through egg-passages is associated with the impediment of viral genome amplification in mouse respiratory cells."Archieves of Virology. 146. 893-908 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 坂口剛正: "エンベロープウイルスのマトリックス蛋白とウイルスの出芽"ウイルス. 51. 11-19 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Noda, M.: "Molecular and epidemiological analyses of human adenovirus type 7 strains isolated from the 1995 nationalwide outbreak in Japan."Journal of Clinical Microbiology. 40. 140-145 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakaguchi, T.: "Alteration of Sendai virus morphogenesis and nucleocapsid incorporation by the mutation of cysteine residues of the matrix protein."Journal of Virology. 76. 1682-1690 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tanji, H.: "Augmentation of local antitumor immunity in liver by interleukin-2 gene transfer via portal vein."Cancer Gene Therapy. 9. 655-664 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujii, Y.: "Involvement of the leader sequence in Sendai virus pathogenesis revealed by recovery of a pathogenic field isolate from cDNA."Journal of Virology. 76. 8540-8547 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukuhara, N.: "Mutational analysis of the Sendai virus V protein: importance of the conserved residues for Zn binding, virus pathogenesis and efficient RNA editing."Virology. 299. 172-181 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujii, Y.: "Identification of mutations associated with attenuation of virulence of a field Sendai virus isolate by egg passage."Virus Genes. 25. 189-193 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 坂口剛正: "抗インフルエンザ薬"カレントテラピー. 20. 56-59 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujii, Y.: "Selective incorporation of influenza virus RNA segments into virions."Proceedings of the National Academy of Sciences of U.S.A.. 100. 2002-2007 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakaguchi, T.: "Masking of the contribution of V protein to Sendai virus pathogenesis in an infection model with a highly virulent field isolate."Virology. 313. 581-587 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 吉田哲也: "ウイルス感染症における臓器特異性"病理と臨床. 21. 45-56 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sugahara, F.: "Paramyxovirus Sendai virus-particle formation by expression of multiple viral proteins and acceleration of its release by C protein."Virology. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakaguchi, T.: "Functional divergence of the Paramyxovirinae accessory proteins : the V and C proteins. in 'Recent Research Developments in Virology Vol.3'"Transworld Network. 341-348 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Huang, C.: "Involvement of the zinc-binding capacity of Sendai virus V protein in viral pathogenesis."Journal of Virology. 74. 7834-7841 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Eguchi, A.: "Identification and characterization of cell lines with a defect in a post-adsorption stage of Sendai virus-mediated membrane fusion."Journal of Biological Chemistry. 275. 17549-17555 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakaguchi, T.: "Therapy by antiviral drugs. (in Japanese)"Kokyu to Junkan. 48. 1127-1131 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujii, Y.: "Conserved and non-conserved regions in the Sendai virus genome : evolution of a gene possessing overlapping reading frames."Virus Genes. 22. 1127-1131 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fan, X.-H.: "Emergence of anti-inflammatory monocytes in long-term surviving host of IL-10-transduced liver allografts."Cytokine. 13. 183-187 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kiyotani, K.: "Attenuation of a field Sendai virus isolate through egg-passages is associated with the impediment of viral genome amplification in mouse respiratory cells."Archieves of Virology. 146. 893-908 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakaguchi, T.: "The matrix protein of enveloped viruses and viral budding. (in Japanese)"Uirusu. 51. 11-19 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakaguchi, T: "Functional divergence of the Paramyxovirinae accessory proteins : the V and C proteins."Recent Research Developments in Virology (ISBN: 81-86846-99-9) (Transworld Network). 3. 341-348 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Noda, M.: "Molecular and epidemiological analyses of human adenovirus type 7 strains isolated from the 1995 nationalwide outbreak in Japan."Journal of Clinical Microbiology. 40. 140-145 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakaguchi, T.: "Alteration of Sendai virus morphogenesis and nucleocapsid incorporation by the mutation of cysteine residues of the matrix protein."Journal of Virology. 76. 1682-1690 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tanji, H.: "Augmentation of local antitumor immunity in liver by interleukin-2 gene transfer via portal vein."Cancer Gene Therapy. 9. 655-664 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujii, Y.: "Involvement of the leader sequence in Sendai virus pathogenesis revealed by recovery of a pathogenic field isolate from cDNA."Journal of Virology. 76. 8540-8547 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fukuhara, N.: "Mutational analysis of the Sendai virus V protein : importance of the conserved residues for Zn binding, virus pathogenesis and efficient RNA editing."Virology. 299. 172-181 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujii, Y.: "Identification of mutations associated with attenuation of virulence of a field Sendai virus isolate by egg passage."Virus Genes. 25. 189-193 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujii, Y.: "Selective incorporation of influenza virus RNA segments into virions."Proceedings of the National Academy of Sciences of U.S.A.. 100. 2002-2007 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakaguchi, T.: "Anti-influenza virus drugs. (in Japanese)"Current Therapy. 20. 56-59 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sakaguchi, T.: "Masking of the contribution of V protein to Sendai virus pathogenesis in an infection model with a highly virulent field isolate."Virology. 313. 581-587 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshida, T.: "Organ tropism in viral infectious diseases. (in Japanese)"Byori to Rinsho. 21. 45-56 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sugahara, F.: "Paramyxovirus Sendai virus-particle formation by expression of multiple viral proteins and acceleration of its release by C protein."Virology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Cheng Huang: "involvement of the zinc-binding capacity of Sendai virus V protein in viral pathogenesis"Journal of Virology. 71(17). 7834-7841 (2000)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yutaka Fujii: "Conserved and non-conserved regions in the Sendai virus genome : evolution of a gene possessing overlapping reading frame"Virus Genes. 22(1). 47-52 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Katsuhiro Kiyotani: "Attenuation of of a field Sendai virus isolate through egg-passages is associated with the impediment of viral genome replication in mouse respiratory cells"Archieves of Virology. 146. 893-908 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] 坂口剛正: "エンベロープウイルスのマトリックス蛋白とウイルスの出芽"ウイルス. 51. 11-19 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] 福原徳子: "センダイウイルスV蛋白に高度に保存されたアミノ酸のウイルス病原性とRNA editingにおける重要性"広島大学医学雑誌. 50(1). 1-12 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takemasa Sakaguchi: "Alteration of Sendai virus morphogenesis and nucleocapsid incorporation by the mutation cysteine residues of the matrix protein"Journal of Virology. 76(4). 1682-1690 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yutaka Fujii: "Involvement of the leader sequence in Sendai virus pathogenesis revealed by recovery of a pathogenic field isolate from cDNA"Journal of Virology. 76(17). 8540-8547 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Noriko Fukuhara: "Mutational analysis of the Sendai virus V protein : inportance of the conserved residues for Zn binding, virus pathogenesis and efficient RNA editing"Virology. 299. 172-181 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yutaka Fujii: "Identification of mutations associated with attenuation of virulence of a field Sendai virus isolate by egg passage"Virus Genes. 25. 189-193 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yutaka Fujii: "Selective incorporation of influenza virus RNA segments into virions"PNAS. 100(4). 2002-2007 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 吉田哲也: "ウイルス感染症における臓器特異性"病理と臨床. 21・臨時増刊. 45-56 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takemasa Sakaguchi: "Masking of the contribution of V protein to Sendai virus pathogenesis in an infection model with a highly virulent field isolate"Virology. 313. 581-587 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takemasa Sakaguchi: "Recent Research Developments in Virology, Vol.3"Functional divergence of the Paramyxovirus accessory proteins: the V and C proteins. 341-348 (2001)

    • Related Report
      2003 Annual Research Report
  • [Publications] Cheng Huang: "Involvement of the zinc-binding capacity of Sendai virus V protein in viral pathogenesis"Journal of Virology. 71(17). 7834-7841 (2000)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yutaka Fujii: "Conserved and non-conserved regions in the Sendai virus genome : evolution of a gene possessing overlapping reading frames"Virus Genes. 22(1). 47-52 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Katsuhiro Kiyotani: "Attenuation of a field Sendai virus isolate through egg-passages is associated with the impediment of viral genome replication in mouse respiratory cells"Archieves of Virology. 146. 893-908 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 坂口剛正: "エンベロープウイルスのマトリックス蛋白とウイルスの出芽"ウイルス. 51. 11-19 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 福原徳子: "センダイウイルスV蛋白に高度に保存されたアミノ酸のウイルス病原性とRNA editingにおける重要性"広島大学医学雑誌. 50(1). 1-12 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takemasa Sakaguchi: "Alteration of Sendai virus morphogenesis and nucleocapsid incorporation by the mutation of cysteine residues of the matrix protein"Journal of Virology. 76(4). 1682-1690 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yutaka Fujii: "Involvement of the leader sequence in Sendai virus pathogenesis revealed by recovery of a pathogenic field isolate from cDNA"Journal of Virology. 76(17). 8540-8547 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Noriko Fukuhara: "Mutational analysis of the Sendai virus V protein : importance of the conserved residues for Zn binding, virus pathogenesis and efficient RNA editing"Virology. 299. 172-181 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yutaka Fujii: "Identification of mutations associated with attenuation of virulence of a field Sendai virus isolate by egg passage"Virus Genes. 25. 189-193 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takemasa Sakaguchi: "Recent Research Developments in Virology Vol. 3"Functinal divergence of the Paramyxovirinae accessory proteins : the V and C proteins. 341-348 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Cheng Huang: "Involvement of the zinc-binding capacity of Sendai virus V protein in viral pathogenesis"Journal of Virology. 74(17). 7834-7841 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yutaka Fujii: "Conseved and non-conserved regions in the Sendai virus genome : evolution of a gene possessing overlapping reading frames"Virus Genes. 22(1). 47-52 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Katsuhiro Kiyotani: "Attenuation of a field Sendai virus isolate through egg-passages is associated with the impediment of viral genome replication in mouse respiratory cells"Archieves of Virology. 146. 893-908 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 福原徳子: "センダイウイルスV蛋白に高度に保存されたアミノ酸のウイルス病原性とRNA editingにおける重要性"広島大学医学雑誌. 50(1). 1-12 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Cheng Huang: "Involvement of the zinc-binding capacity of Sendai virus V protein in viral pathogenesis."Journal of Virology. 74(17). 7834-7841 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yutaka Fujii: "Conserved and non-conserved regions in the Sendai virus genome : evolution of a gene possessing overlapping reading frames."Virus Genes. 22(1). 47-52 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Katsuhiro Kiyotani: "Attenuation of a field Sendai virus isolate through egg-passages is associated with the impediment of viral genome replication in mouse respiratory cells."Archives of Virology. (in press). (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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