| Project/Area Number |
12470085
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | Yamaguchi University |
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Principal Investigator |
HOBARA Tatsuya Yamaguchi University School of Medicine, Professor, 医学部, 教授 (10116501)
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| Co-Investigator(Kenkyū-buntansha) |
KUNITSUGU Ichiro Yamaguchi University School of Medicine, Research associate, 医学部, 助手 (80314798)
OKUDA Masayuki Yamaguchi University School of Medicine, Associate Professor, 医学部, 助教授 (50274171)
SHINODA Koh Yamaguchi University School of Medicine, Professor, 医学部, 教授 (40192108)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥11,900,000 (Direct Cost: ¥11,900,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥10,900,000 (Direct Cost: ¥10,900,000)
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| Keywords | intracellular calcium / trchloroethylene / trichloroethane / Fluo-3 / bromopropane / neuron / rat / caffeine / カルシウムチャンネル / 細胞内カルシウムイオン / パッチクランプ / 血管平滑筋 / 脊髄後根神経節細胞 / 有機溶剤 / ヘキサン / Fura-2 |
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| Research Abstract |
Calcium ion in neurons plays an important role in development of action potential, release of neurotransmitter and intracellular signaling with synapse formation and physiological functions. Intracellular calcium ion is derived from either influx from the extracellular fluid through the membrane calcium channels o* release out of the intracellular calcium store sites. Organic solvents used in the field of manufacturing and service industry cause clinical symptoms related to depression of central nervous system function.
We have dissociated dorsal root ganglion neurons from neonatal rat to measure the voltage-dependent calcium current in a whole cell mode of the patch clump. Trichloroethane and trichloroethyene decreased the calcium current. And intervals of the action potential in the current clump mode were shortened. We also found the involvement of other voltage-gated cation channels. Next we investigated the change of intracellular calcium ion concentration released from the intracelular calcium stores with a fluo-indicator Fluo-3. We used in this experiments caffeine which modifies ryanodine receptor of intracellular calcium store, and ATP which stimulates the nucleotide receptor of the DRG neurons. Caffeine induced the increasing of the intracellular calcium level, and trichloroethylene inhibited the increasing. The rise of the intracellular calcium level was also impaired with 1-bromopropane and 2-bromopropane. We also studied aortic smooth muscle and got the same results. These studies suggested that some of organic solvents could act on both the plasma membrane of the neurons and cytoplasmic organelles in entering though the plasma membrane and finally modify the action potential and neurotransmitter release.
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