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Construction of a new method for Molecular epidemiology of tuberculosis by using DNA micro aray

Research Project

Project/Area Number 12470105
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Public health/Health science
Research InstitutionTottori University Faculty of medicine (2003)
Osaka Prefectural Institute of Public Health (2000-2002)

Principal Investigator

SUZUKI Yashihiko  Tottori University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (90206540)

Co-Investigator(Kenkyū-buntansha) TAMARU Aki  Osaka Prefectural Institute of Public Health, Researcher, 感染症部, 研究員 (70270767)
MATSUBA Takashi  Tottori University, Faculty of medicine, Lecturer, 医学部, 講師 (20304206)
織田 肇  大阪府立公衆衛生研究所, 労働衛生部, 部長兼副所長 (00132845)
Project Period (FY) 2000 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥12,900,000 (Direct Cost: ¥12,900,000)
Fiscal Year 2003: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2002: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2001: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥4,400,000 (Direct Cost: ¥4,400,000)
KeywordsMycobacterium tuberculosis / molecular epidemiology / inter IS6110 PCR / ipl / DNAジャイレース / 直位反復配列 / スペーサーオリゴヌクレオチド / IS6110優先挿入領域 / PCR / 挿入配列 / セルフライゲーションPCR / ポリカルボジイミド樹脂 / DNAチップ / ダイレクトリピート / ハイブリダイゼーション / 疫学的解析法
Research Abstract

We have investigated followings
1)A new method for the rapid typing of clinically isolated Mycobacterium tuberculosis strains was constructed and termed as self-ligation mediated polymerase chain reaction (SL-PCR)
2)Extended spacer oligonucleotide DNA micro array was established and the performance was compared with conventional spoligotyping method. Extended spacer oligonucleotide DNA micro array could discriminate the strains that can not be discriminated by spoligotyping method.
3)The IS6110 preferential locus (ipl) of clinically isolated M.tuberculosis strains in Japan were analyzed to lack the diversity. However, the analysis of this locus turned out to be utilized for the molecular epidemiology of tuberculosis.
4)The gyrA amd gyrB that are coding for DNA gyrase A subunit and B subunit, respectively of clinically isolated M.tuberculosis strains in Japan were analyzed. The Japanese clinical isolates could be divided into two groups by the polymorphysm of these genes.
5)By amplifying the intervening sequence between IS6110 with inter IS6110 PCR, about 80 % of clinically Isolated M.tuberculosis strains in Japan were classified. This turned out to be applied for the construction of new DNA micro array for molecular epidemiology of tuberculosis.

Report

(5 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Yoda T., Suzuki Y., Terano Y.et al.: "Precise Characterization of Norovirus (Norwalk-Like Virus)-Specific Monoclonal Antibodies with Broad Reactivity."J.Glin.Microbiol.. 41. 2367-2371 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzuki K, Suzuki Y, Kitamura S.: "Cloning and expression of a UDP-glucuronic acid decarboxylase gene in rice."J Exp Bot.. 54. 1997-1999 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Agdamag DM, Kageyama S, Suzuki Y. et al.: "Characterization of clinical isolates of Mycobacterium tuberculosis resistant to drugs and detection of RpoB mutation in multidrug-resistant tuberculosis in the Philippines."Int J Tuberc Lung Dis.. 7. 1104-1108 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoda T., Suzuki Y., Terano Y.et al.: "Precise Characterization of Norovirus (Norwalk-Like Virus)-Speciflc Monoclonal Antibodies with Broad Reactivity"J.Clin.Microbiol. 41. 2367-2371 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Suzuki K, Suzuki Y, Kitamura S.: "Cloning and expression of a UDP-glucuronic acid decarboxylase gene in rice."J Exp Bot.. 54. 1997-1999 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Agdamag DM, Kageyama S, Suzuki Y.et al.: "Characterization of clinical isolates of Mycobacterium tuberculosis resistant to drugs and detection of RpoB mutation in multidrug-resistant tuberculosis in the Philippines."Int J Tuberc Lung Dis.. 7. 1104-1108 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoda T., Suzuki Y., Terano Y., et al.: "Precise Characterization of Norovirus (Norwalk-Like Virus)-Specific Monoclonal Antibodies with Broad Reactivity"J.Clin.Microbiol.. 41. 2367-2371 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suzuki K, Suzuki Y, Kitamura S.: "Cloning and expression of a UDP-glucuronic acid decarboxylase gene in rice"J Exp Bot.. 54. 1997-1999 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Agdamag DM, Kageyama S., Suzuki Y., et al.: "Characterization of clinical isolates of Mycobacterium tuberculosis resistant to drugs and detection of RpoB mutation in multidrug-resistant tuberculosis in the Philippines"Int J Tuberc Lung Dis.. 7. 1104-1108 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suzuki Y., Suzuki A., Tamaru A., et al.: "Rapid detection of pyrazinamide resistant Mycobacterium tuberculosis by PCR based in vitro system"J.Clin.Microbiol.. 40. 501-507 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ahao H., Wakamiya N., Suzuki Y., et al.: "Identification of human monnose binding lectin (MBL) recognition sites for novel inhibitory antibodies"Hybridoma and Hybridomics. 21. 25-36 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kawai T., Suzuki Y., Yamashita A., et al.: "Isolation of a novel mouse variant of the drs tumor suppressor gene"Cancer Lett.. 183. 79-86 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kawai T., Suzuki Y., Eda S., et al.: "Molecular cloning of mouse Collectin Liver 1"Biosci.Biotechnol.Biochem.. 66. 2134-2145 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shimada T., Inoue K., Suzuki Y., et al.: "Arylhydrocarbon receptor-dependent induction of liver and lung cytochromes P450 1A1, 1A2, and 1B1 by polycyclic aromatic....."Carcinogenesis. 23. 1199-1207 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 若宮伸隆, 鈴木定彦: "MBLその機能と生理学的意義"臨床病理中四会誌. 17. 2-12 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suzuki Y., Suzuki A., Tamaru A., et al.: "Rapid detection of pyrazinamide resistant Mycobacterium tuberculosis by PCR based in vitro system"J. Clin. Microblol.. 40. 501-507 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ohtani K, Suzuki Y, Eda S et al.: "The membrane type collectin CL-P1 is a scavenger receptor on vascular endothelial cells"J. Biol. Chem.. 276. 44222-44228 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoda T., Terano Y., Suzuki Y.et al.: "Characterization of Norwalk virus GI specific monoclonal antibodies generated against Escherichia coli expressed capsid protein and reactivity of two …"BMC Micobiol.. 1. 24 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tsuji S, Uehori J, Matsumoto M, Suzuki Y et al.: "Hunan intelectin is a novel soluble lectin that recognizes galactofuranose in carbohydrate chains of bacterial cell-wall"J Biol. Chem.. 276. 23456-23463 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 鈴木定彦, 田丸亜貴, 鈴木文, 勝川千尋: "結核菌の薬剤耐性"感染症. 31. 75-83 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 鈴水定彦, 若宮伸隆: "感染防御とコレクチンファミリー"Annual Review 免疫2002. 217-225 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ruhul,A.,Suzuki,Y.,Takatorige,T.,Tatara,K.,Shirakura,R.: "Usefulness of self ligation mediated polymerase chain reaction : A rapid method for fingerprinting in molecular epidemiology of tuberculosis"Kekkaku. 76(1). 9-18 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 鈴木定彦,市原竜生,田丸亜貴,Amin Ruhul,勝川千尋 他: "DNAチップによる結核菌の耐性診断"Bioindustry. 17(5). 36-41 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 鈴木定彦,田丸亜貴,Amin Ruhul,勝川千尋: "結核菌の薬剤耐性獲得機序の解析と迅速診断への展開"防菌防徽. 28(9). 561-573 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 長谷川好規,阿部千代治,飯沼山嗣,鈴木定彦,高橋光良,水口康雄: "結核-分子遺伝学からのアプローチ"結核. 75(12). 725-728 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 鈴木定彦,田丸亜貴,鈴木文,勝川千尋: "細菌の化学療法剤と薬剤耐性(結核菌)"臨床と微生物. 28(1). 35-41 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Suzuki Y,Yoda T,Ruhul A,Sugiura W: "Molecular cloning and characterization of the gene coding for azoreductae from Bacillus sp.OY 1-2 isolated from soil."J.Biol.Chem. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 鈴木定彦,市原竜生,田丸亜貴,Amin Ruhul,勝川千尋 他: "DNAチップ応用技術、第15章 結核菌の薬剤耐性"監修 松永 是,発行者 島 健太郎,第15章執筆. 12(225) (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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