| Project/Area Number |
12470113
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YAMAMOTO Kazuhiko University of Tokyo, Faculty of Medicine, Professor, 医学部・附属病院, 教授 (80191394)
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| Co-Investigator(Kenkyū-buntansha) |
DOHI Makoto University of Tokyo, Faculty of Medicine, Assistant, 医学部・附属病院, 助手 (60222155)
MISAKI Yoshikata University of Tokyo, Faculty of Medicine, Lecturer, 医学部・附属病院, 講師 (60219615)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥15,500,000 (Direct Cost: ¥15,500,000)
Fiscal Year 2001: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 2000: ¥9,500,000 (Direct Cost: ¥9,500,000)
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| Keywords | Oral tolerance / T cells / IL-10 / Peyer's patch / dendritic cells |
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| Research Abstract |
Oral tolerance is one of the promising methods for immunotherapy. Regulatory T cells generated by the orally administered antigens would be the most responsible cell population for the suppressive action. In order to establish regulatory T cell lines, it is important to know the mechanisms of the regulation as well as the conditions to generate these T cells. We thus establish an assay system to monitor the suppressive activity to the ongoing immune-response by mixing the candidate regulatory cells with antigen-specific splenocytes. We next focused on the Th3/Tr1 cells which secrete TGF-beta because this cells is one of the most well characterized regulatory cells. We have thus characterize the several culture conditions to generate Th3/Tr1 cells. Spleen cells from OVA specific TCR transgenic mice were cultured in different conditions and resultant OVA specific cells were assayed for the secretion of TGF-beta or the above established evaluation system. We are now narrowing down suitable conditions for the generation of regulatory cells.
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