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Gene expression profiling of biliary epithelial cells in primary biliary cirrhosis : laser microdissection and microarray analysis

Research Project

Project/Area Number 12470122
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionOkayama University

Principal Investigator

YAMAMOTO Kazuhide  Okayama University Hospital, Lecturer, 医学部附属病院, 講師 (90140491)

Co-Investigator(Kenkyū-buntansha) NINOMIYA Yoshifumi  Okayama University Graduate School of Medicine and Dentistry, Professer, 大学院・医歯学総合研究科, 教授 (70126241)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥14,500,000 (Direct Cost: ¥14,500,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥12,000,000 (Direct Cost: ¥12,000,000)
KeywordsPrimary biliary cirrhosis / Laser microdissection / Biliary epithelial cells / DNA microarray / DNA マイクロアレイ
Research Abstract

Although primary biliary cirrhosis (PBC) is an organ-specific autoimmune disease, target molecules remains unidentified. We attempted to identify target molecules in biliary epithelial cells of PBC by laser capture microdissection (LCM) and micro-array technology.
Preliminary experiments showed successful collection of pure biliary epithelial cells and amplification of messages by a T7 RNA polymerase based method.
Gene expression profiles of biliary epithelial cells were compared among normal, chronic hepatitis C and PBC patients. Biliary epithelial cells from PBC patients demonstrated increased expression of some genes compared with normal biliary cells.
However, some of the genes showing increased expression were also increased in biliary epithelial cells from chronic hepatitis C patients, suggesting that the increased expression might be induced by portal inflammation. Analysis of PBC specific gene expressions is now under study.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Okamoto R, et al.: "T Cell Repertoire in Primary Biliary Cirrhosis : A Common T Cell Clone and Repertoire Change After Treatment"J Clin Immunol. 21. 278-285 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] matsumura S, et al.: "Contribution to antimitochondrial antibody production : cleavage of pyruvate dehydrogenase complex-E2 by apoptosis-related prot"Hepatology. 35. 14-22 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsumura S, et al.: "Comprehensive mapping of HLA-A0201-restricted CD8 T-cell epitopes on PDC-E2 in primary biliary cirrhosis"Hepatology. 36. 1125-1134 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Terada R, et al.: "Stromal cell derived factor-1 from biliary epithelial cells recruits CXCR4-positive cells"Lab Invest. (In press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamamoto K, et al.: "A scoring system for primary biliary cirrhosis and its application for variant forms of autoimmune liver disease"J Gastroenterol. 38. 52-59 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okamoto R, et al.: "T cell repertoire in primary biliary cirrhosis"J Clin Immunol.. 21. 278-285 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsumura S, et al.: "Contribution to antimitochondrial antibody production"Hepatology. 35. 14-22 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsumura S, et al.: "Comprehensive mapping of HLA-A0201-restricted CD8 T-cell epitopes"Hepatology. 36. 1125-1134 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Terada R, et. Al.: "Stromal cell derived factor-1 from biliary epithelial cells"(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamamoto K, et al.: "A scoring system for primary biliary cirrhosis and its application"J Gastroenterol. 38. 52-59 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsumura S, et al.: "Comprehensive mapping of HLA-A0201-restricted CD8 T-cell epitopes on PDC-E2 in primary biliary cirrhosis"Hepatology. 36(5). 1125-1134 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamamoto K, et al.: "A scoring system for primary biliary cirrhosis and its application for variant forms of autoimmune liver disease"J Gastroenterol. 38(1). 52-59 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Terada R, et al.: "Stromal cell-derived factor-1 from biliary epithelial cells recruits CXCR4-positive cells : implications for inflammatory liver diseases"Lab Invest. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Matsumura S, et al.: "Contribution to antimitochondrial antibody production : cleavage of pyru vate dehydrogenase complex-E2 by apoptosis-related proteases"Hapatology. 35・1. 14-22 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ibuki N, et al.: "In situ expression of granzyme B and Fas-lignad in the liver of viral hepatitis"Liver. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Okamoto R,Yamamoto K, et al.: "T Cell Repertoire in Primary Biliary Cirrhosis : A Common T Cell Clone and Repertoire Change After Treatment"J Clin Immunol. (In press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Matsumura S,Yamamoto K, et al.: "High Frequency of Circulating HBcAg-Specific CD8 T cells in Hepatitis B Infection : A Flow Cytometric Analysis"Clin and Exp Immunol. (In press).

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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