| Project/Area Number |
12470130
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
SAKAMOTO Choitsu Nippon Medical School, Third Department of Internal Medicine, Professor, 医学部, 教授 (30196092)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMORI Takahiro Dokkyo University School of Medicine, Department of Pathology, Professor, 医学部, 教授 (30095385)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥14,300,000 (Direct Cost: ¥14,300,000)
Fiscal Year 2001: ¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 2000: ¥8,800,000 (Direct Cost: ¥8,800,000)
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| Keywords | EGF / follistatin / stomach fibroblast / colon cancer / tomoregulin / erbB2 / erbB4 / fomoregulin / fomokegulin |
|---|
| Research Abstract |
We have isolated a gene from stomach fibroblasts encoding novel proteins containing two follistatin modules and a single EGF-like domain. We designated the protein products of a gene tomoregulin (TR). Highly purified TR by using affinity chromatography with a monoclonal antibody from Sf9 cells stimulated tyrosine phosphorylation of erbB4, but not erbB2, erbB3, and EGF receptors in MKN28 gastric cancer cells, while TR inhibited MKN-1 gastric cancer cell growth without affecting erbB4 phosphorylation. TR-EGF domain also inhibited MKN-1 growth, suggesting that EGF domain of TR, but not follistatin modules, plays a role for the inhibition. TR or TR-EGF domain stimulated phosphorylation of MAP kinase p44 and p42 in MKN-1 gastric cancer cells without affecting erbB4 phosphorylation, suggesting that TR binds to another erbB receptor not yet known, exerting its effect on cell growth inhibition and MAP-kinase phosphorylation.
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