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Molecntan biological and pharmaco-physiological approach to pathophysiological roles of endothelin-1 in aggrarating heart failure

Research Project

Project/Area Number 12470147
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionUniversity of Tsukuba

Principal Investigator

MIYAUCHI Takashi  University of Tsukuba, Professon of Institute of Clinical Medicine, 教授 (60222329)

Co-Investigator(Kenkyū-buntansha) GOTO Katsutoshi  University of Tsukuba, Professon of Institute of Basic Medical Sciences, 教授 (30012660)
SAKURAI Takeshi  University of Tsukuba, Associate Professon of Institute of Basic Medical Sciences, 助教授 (60251055)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2002: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2000: ¥5,700,000 (Direct Cost: ¥5,700,000)
Keywordsheart failare / endothelin / antagonist / pharmacology / phtsiology / mclesular biology / gene expression / cardiac hypeitrsphy / 分子循環器学 / 薬理学 / 病態生理学 / エネルギー代謝 / 循環薬理学 / 病状悪化
Research Abstract

In the present study, we studied the pathophysiological roles of endothelin-1 (ET-1) in heart failure by molecular biological and pharmaco-physiological approach. ET-1 induces myocardial hypertrophy and causes cellular injury in cardiac myocytes. The production of ET-1 is markedly increased in the failing heart and chronic treatment with an ET receptor antagonist greatly improves the survival rate of animals with CHF. An ET antagonist improves the alteration in the expression of various cardiac genes of classic molecular markers (eg, mRNA in ANP and 13 -myosin heavy chain) and of functional molecular markers (eg, mRNA levels of ryanodine receptor, sarcoplasmic reticulum calcium-ATPase) in the failing heart, suggesting that the great improvement of survival in CHF animals by an ET antagonist is partly attributed to the prevention of molecular changes in the failing heart. Several molecular mechanisms are considered to be involved in the marked increase in ET-1 expression in the failing heart. The transcription of the ET-1 gene is regulated through the phorbol-ester-sensitive c-fos and c-jun complexes, binding sites for nuclear factor-1, AP-1, and GATA proteins. In the failing heart, principal ATP generation by mitochondrial β-oxidation is impaired and adaptively switched to glycolysis. Hypoxia-inducible factor (HIF)-1α is an important transcriptional factor which activates gene expression of glycolytic enzymes. We find a HIF-1α binding site in 5'-regulatory region of ET-1 gene. Our data revealed a novel molecular mechanism of up-regulation of cardiac ET-1 in heart failure that the impairment of cardiac energy metabolism is involved in a marked increase in ET-1 expression through a transcriptional activation by HIF-1α. We also revealed that ET-1 induced cardiac hypertrophy is inhibited by activation of PPAR a partly via c-Jun NH2-terminal-kinase pathway. The present study showed that ET-1 plays important roles in worsening heart failure.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (35 results)

All 2002 2001 Other

All Journal Article (13 results) Book (1 results) Publications (21 results)

  • [Journal Article] A combination of oral endothelin-A receptor antagonist and oral prostacyclin analogue is superior to each drug alone in ameliorating pulmonary hypertension in rats2002

    • Author(s)
      Ueno M, Miyauchi T, et al.
    • Journal Title

      Journal of the American College of Cardiology vol 40

      Pages: 175-181

    • NAID

      10010226459

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Vascular endothelin-B recepton system in vivo plays a favahable inhibitory role in vascular remodeling after injury revealed by endothelin-B receptor Knockoutmice2002

    • Author(s)
      Murakoshi N, Miyauchi T, et al.
    • Journal Title

      Circulation vol.106

      Pages: 1991-1998

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Involvement of endogenour endothelin-1 in exercise-induced redistribution of tissue blood flow : An endothelin receptor antagonist reduces the redistribatier2002

    • Author(s)
      Maeda S, Miyauchi T, et al.
    • Journal Title

      Circulation vol.106

      Pages: 2188-2193

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] A combination of oral endothelin-A receptor antagonist and oral prostacyclin analogue is superior to each drug alone in ameliorating pulmonary hypertension in rats2002

    • Author(s)
      Ueno M, Miyauchi T, Sakai S, Yamauchi-Kohno R, Goto K, Yamaguchi I
    • Journal Title

      Journal of the American College of Cardiology 40(1)

      Pages: 175-181

    • NAID

      10010226459

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Vascular endothelin-B receptor system in vivo plays a favorable inhibitory role in vascular remodeling after injury revealed by endothelin-B receptor-knockout mice.2002

    • Author(s)
      Murakoshi N, Miyauchi T, Kakinuma Y, Ohuchi T, Goto K, Yanagisawa M, Yamaguchi I
    • Journal Title

      Circulation 106(15)

      Pages: 1991-1998

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Involvement of endogenous endothelin-1 in exercise-induced redistribution of tissue blood flow An endothelin receptor antagonist reduces the redistribution.2002

    • Author(s)
      Maeda S, Miyauchi T, Iemitsu M, Tanabe T, Irukayama-Tomobe Y, Goto K, Yamaguchi I, Matsuda M
    • Journal Title

      Circulation 106(17)

      Pages: 2188-2193

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Aging-induced decrease in the PPAR-α level in hearts is improved by exercise training.2002

    • Author(s)
      Iemitsu M, Miyauchi T, Maeda S, Tanabe T, Takanashi M, Irukayama-Tomobe Y, Sakai S, Ohmori H, Matsuda M, Yamaguchi I
    • Journal Title

      American Journal of Physiology 283(5)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] A novel pharmacological action of ET-1 to prevent the cytotoxicity of doxorubicin in cardiom yocytes2001

    • Author(s)
      Suzuki T, Miyauchi T.
    • Journal Title

      American Journal of Physiology vol280

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Novel molecular mechanism of increased myocardial endothelin-1 expression in the failing heart involving the transcriptional factor hypoxia-indacible factor-1α induced for impaired myocardical enargy metabolism2001

    • Author(s)
      Kakinuma Y, Miyanchi T, et al.
    • Journal Title

      Circulation vol 103

      Pages: 2387-2394

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Physiological and pathological cardiac hypertrophy induce different molecular phenotypes in the rat2001

    • Author(s)
      Iemitsu M, Miyauchi T, et. al.
    • Journal Title

      American Journal of Physiology vol. 281

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] A novel pharmacological action of ET-1 to prevent the cytotoxicity of doxorubicin in cardiomyocytes.2001

    • Author(s)
      Suzuki T, Miyauchi T
    • Journal Title

      American Journal of Physiology 280

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Novel molecular mechanism of increased myocardial endothelin-1 expression in the failing heart involving the transcriptional factor hypoxia-inducible factor-1α induced for impaired myocardial energy metabolism.2001

    • Author(s)
      Kakinuma Y, Miyauchi T, Yuki K, Murakoshi N, Goto K, Yamguchi I
    • Journal Title

      Circulation 103(19)

      Pages: 2387-2394

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Physiological and pathological cardiac hypertrophy induce different nalecular phenotypes in the rat.2001

    • Author(s)
      Iemitsu M, Miyauchi T, Maeda S, Sakai S, Kobayashi T, Fujii N, Miyazaki H, Matsuda M, Yamaguchi I
    • Journal Title

      American Journal of Physiology 281(6)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Book] Annual Review 2002循環器2002

    • Author(s)
      酒井俊, 宮内卓
    • Total Pages
      282
    • Publisher
      中外医学社(編集者:杉下靖郎,門間和夫,矢崎義雄,高本眞一)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Murakoshi N, Miyauchi T, et al.: "Vascular endothelin-B receptor system in vivo plays a favorable inhibitory role in vascular remodeling after injury revealed by endothelin-B receptor knockout mice"Circulation. Vol.106 No.15. 1991-1998 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Maeda S, Miyauchi T, et al.: "Involvement of endogenaus endothelin-1 in exercise-induced redistribution of tissue blood flow : An endothelin receptor antagonist reduces the redistributio"Circulation. Vol.106 No.17. 2188-2193 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ueno M, Miyauchi T, et al.: "A combination of oral endothlin-A receptor antagonist and oral prostacyclin andogue is superior to each dung alone in ameliorating pulmorary hypertension in rats"Journal of the American College of Cardiology. Vol.40 No.1. 175-181 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Iemitsu M, Miyauchi T, et al.: "Aging-induced decrease in the PPAR-α level in hearts is improved by exercise training"American Journal of Physiology. Vol.283 No.5. H1750-H1760 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ogata T, Miyauchi T, et al.: "Stimulation of peroxisome-prdiferotor-activated receptor α(PPARα)attenuates cardiac fibrosis and endothelin-1 production in pressure-overlooded rat hearts"Clinical Science. Vol.103 Suppl.48. 284S-288S (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Miyauchi T.et al.: "Exercise causes a tissue-specific change of NO production in the kidney and lung"Journal of Applied Physiology. Vol.94. 60-68 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kahinuma Y., Miyauchi T., et al.: "Novel molecular mechanism of increased myocardial endothelin-1 exression in the failing heart involving the transcriptional factor hypoxia-inducible factor-2α inducel for myocardial energy metabolism"Circulation. vol103. 2387-2394 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yuki K., Miyauchi T., et al.: "Endothelin-1 production is enhanced by a rotenon,a mitochondrial complex I inhibitor,in cultured rat cardiomyocytes"Journal of Cardiovascular Pharmacology. vol38. 850-858 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Suzuki T., Miyauchi T.: "A novel pharmacological action of ET-1 to prereut the cyto toxicity of boxorubicin in cardiomyocytes"American Journal of Physiology. vol280. R1399-R1406 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kumagai Y., Hayashi T., Miyauchi T., et al.: "Phenanthra quivovl inhibits eNOS activity and suppresses vasorelaxation"American Journal of Physiology. vol281. R25-R30 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Maeda S., Miyauchi T., et al.: "Effects of exercise training of 8 weeks and detraining on plasma levels of endothelium- derirel factors,endotheliu-1 and nitric oxide in healthy young humans"Life Sciences. vol69. 1005-1016 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Iemitsu M., Miyauchi T., et al.: "Physiological and pathological cardiac hypertrophy induce different molecular phenotypes in the rat"American Journal of Physiology. vol281. R2029-R2036 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 前田清司, 酒井俊, 宮内卓: "心臓における生命現象の分子生物学(小室一成 編)"メディカルレビュー社. 242 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Miyauchi T et al.: "Endothelin -1 and right -sided heart failure in rats."Journal of Cardiovascular Pharmacology. Vol.36. S327-S330 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sakai S,Miyauchi T et al.: "Long -term enaothetin receptor antagonist administration improves alterations in expression of various cardiac gene in failing myocardium of rats with heart failure."Circulation. Vol.101 No.24. 2849-2853 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Murakoshi N,Miyauchi T et al.: "Impairment of cardiac energy metabolism in vivo causes hemodynamic abnormality and increases cardiac expression of preproendothelin -1 mRNA"Journal of Cardiovascular Pharmacology. Vol.36. S128-S131 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kakinuma Y,Miyauchi T et al.: "Mitochondrial dysfunction of cardiomyocytes causing impairment of cellular energy metabolism induces apoptosis, and concomitant increase in cardiac and otheline -1 expression."Journal of Cardiovascular Pharmacology. Vol.36. S201-S204 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Imeitsu M,Miyauchi T et al.: "Intense exercise causes decrease in expression of both enaothelial NO synthase and tissue NOx level in hearts."American Journal of Physiology. Vol.279. R951-R959 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yuki K,Miyauchi T et al.: "Mitochondrial dysfunction increases expression of endothelin -1 and induces apoptosis through caspase -3 activation in rat cardiomyocy in vitro"Journal of Cardiovascular Pharmacology. Vol.36. S205-S208 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Miyauchi T,and Sugishita Y.: "Heart failure ;Frontiers in Cardiology"Springer -Verlag (Edited by A. kitabatake, S. Sasayama, G. S. Francis). 261 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 横田倫子,宮内卓: "高血圧ナビゲーター"メディカルレビュー社(荻原後男編). 286 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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