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Development of the gene therapy technologies using adeno-associated virus (AAV)

Research Project

Project/Area Number 12470203
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionJichi Medical School

Principal Investigator

OZAWA Keiya  Jichi Medical School, Faculty of Medicine, Professor, 医学部, 教授 (30137707)

Co-Investigator(Kenkyū-buntansha) MIZUKAMI Hiroaki  Jichi Med. Sch., Faculty of Medicine, Research Associate, 医学部, 助手 (20311938)
KUME Akihiro  Jichi Med. Sch., Faculty of Medicine, Assistant Professor, 医学部, 助教授 (10264293)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 2001: ¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 2000: ¥8,200,000 (Direct Cost: ¥8,200,000)
KeywordsGene therapy / Gene transfer / AAV vector / packaging cell line / AAVS1 locus / Rep / ITR / TVI method
Research Abstract

We studied the gene transfer methods using adeno-associated virus (AAV).
1. Development of the method for AAV vector production: We developed novel packaging cell lines for AAV vector production by regulating the expression of cytotoxic AAV proteins through Cre/loxP system. First, we applied combined Cre/loxP system using variant loxP and wild-type loxP to the simultaneous regulation of Rep and Cap expressions. Second, we developed a novel 293-derived prepackaging cell line which constitutively expresses the antisense rep/cap driven by a loxP-flanked CMV promoter. This cell line was converted into a packaging cell line expressing Rep/Cap through the introduction of a Cre recombinase gene.
2. Establishment and application of highly sensitive detection method for AAV vector-mediated transgenes : Long PCR (sometimes combined with nested PCR) was conducted with appropriate primer sets located on the D region of ITR. We also collected many samples from the experimental animals which received intramuscular injections of AAV vectors.
3. Development of the method for targeted vector integration (TVI) into a defined locus on chromosome 19 using AAV-derived components (ITR and Rep gene) : 293 and K562 cells were transfected with the neoγ gene using the TVI method. We amplified junctional regions between cellular and transgene sequences by Alu-PCR. As a result, no cellular sequences regarded as a common recognition motif of the Rep proteins was found. We also developed mutant Rep-expression vectors with reduced cytotoxicity.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (92 results)

All Other

All Publications (92 results)

  • [Publications] Hasumi, Y.: "Soluble Flt-1 expression suppresses carcinomatous ascites in nude mice bearing ovarian cancer"Cancer Res.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wang, L.: "Delayed delivery of AAV-GDNF prevents nigral neurodegeneration and promotes functional recovery in a rat model of Parkinson's disease"Gene Ther.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shimpo, M.: "AAV-mediated VEGF gene transfer into skeletal muscle stimulates angiogenesis and improves blood flow in a rat hindlimb ischemia model"Cardiovasc. Res.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Muramatsu, S.: "Behavioral recovery in a primate model of Parkinson's disease by triple transduction of striatal cells with adeno-associated viral vectors expressing dopamine-synthesizing enzymes"Hum. Gene Ther.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Okada, T.: "Adeno-associated viral vector-mediated gene therapy of ischemia-induced neuronal death"Method. Enzymol.. 346. 378-393 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kawada, T.: "Rescue of hereditary form of dilated cardiomyopathy by rAAV-mediated somatic gene therapy : Amelioration of morphological findings, sarcolemmal permeability, cardiac performances, and the prognosis of TO-2 hamsters"Proc. Natl. Acad. Sci. U.S.A.. 99. 901-906 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Okada, T.: "Development and characterization of an antisense-mediated regulation system for adeno-associated virus vector production with introduction of Cre recombinase"Biochem. Biophys. Res. Commun.. 288. 62-68 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Saga, Y.: "Expression of HGF/NK4 in ovarian cancer cells suppresses intraperitoneal dissemination and extends host survival"Gene Ther.. 8. 1450-1455 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Muramatsu, M.: "Reversible integration of the dominant negative retinoid receptor gene for ex vivo expansion of hematopoietic stem/progenitor cells"Biochem. Biophys. Res.. Commun.. 27. 891-896 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maeda, Y.: "Adeno-associated virus-mediated transfer of endothelial nitric oxide synthase gene inhibits protein synthesis of rat ventricular cardiomyocytes"Cardiovas. Drug Ther.. 15. 19-24 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kogure, K.: "Targeted integration of foreign DNA into a defined locus on chromosome 19 in K562 cells using AAV-derived components"Int. J. Hematol.. 73. 469-475 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Xin, K.-Q.: "A novel recombinant adeno-associated virus vaccine induces a long-term humoral immune response to human immunodeficiency virus"Hum. Gene Ther.. 12. 1047-1061 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maeda, Y.: "Adeno-associated virus-mediated transfer of endothelial nitric oxide synthase gene reduces vasoconstrictive response"Exp. Clin. Cardiol.. 6. 50-55 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kawada, T.: "Morphological and physiological restorations of hereditary form of dilated cardiomyopathy by somatic gene therapy"Biochem. Biophys. Res. Commun.. 284. 431-435 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kanazawa, T.: "γ-Rays enhance rAAV-mediated transgene expression and cytocidal effect of AAV-HSVtk/ganciclovir on cancer cells"Cancer Gene Ther.. 8. 99-106 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fan, D.: "Adeno-associated virus vector-mediated triple gene transfer of dopamine synthetic enzymes"Chin. Med. J.. 114. 1276-1279 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shinjyo, T.: "Downregulation of Bim, a proapoptotic relative of Bcl-2, is a pivotal step in cytokine-initiated survival signaling in murine hematopoietic progenitors"Mol. Cell. Biol.. 21. 854-864 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ozawa, K.: "Gene therapy or Parkinson's disease using adeno-associated virus (AAV) vectors"J. Neural Transm. [suppl]. 58. 181-191 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maeda, Y.: "Adeno-associated virus-mediated vascular endothelial growth factor gene transfer into cardiac myocytes"J. Cardiovasc. Pharm.. 36. 438-443 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Urabe, M.: "Self amplification system for recombinant adeno-associated virus production"Biochem. Biophys. Res. Commun.. 276. 559-563 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shen, Y.: "Triple transduction with adeno-associated virus vectors expressing tyrosine hydroxylase, aromatic l-amino acid decarboxylase, and GTP cyctohydrolase l for gene therapy of Parkinson's disease"Hum. Gene Ther.. 11. 1509-1519 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shimpo, M.: "Gene transfer into rat renal cells using adeno-associated virus vectors"Am. J. Nephrol.. 20. 242-247 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shimazaki, K.: "Adeno-associated virus vector-mediated bcl-2 gene transfer into post-ischemic gerbil brain in vivo : prospects for gene therapy of ischemia-induced neuronal death"Gene Ther.. 7. 1244-1249 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kume, A.: "Long-term tracking of murine hematopoietic cells transduced with a bicisronic retrovirus containing CD24 and EGFP genes"Gene Ther.. 7. 1193-1199 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsuda, K.M.: "A novel strategy for the tumor angiogenesis-targeted gene therapy : Generation of angiostatin endogenous plasminogen by protease gene transfer"Cancer Gene They.. 7. 589-596 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Muroi, K.: "Autologous peripheral blood stem cell transplantation for adults with B-lineage acute lymphoblastic leukemia : a pilot study"Leukemia Lymphoma. 38. 103-111 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Urabe, M.: "DNA/calcium phosphate precipitates mixed with medium are stable and maintain high transduction efficiency"Anal. Biochem.. 278. 91-92 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maeda, Y.: "Endogenously generated nitric oxide by nitric-oxide synthase gene transfer inhibits cellular proliferation"J. Phamacol. Exp. Therapeut.. 292. 387-393 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ozawa, K.: "Progress in Gene Therapy-Basic and Clinical Frontiers"VSP BV, Netherlands (ed. by Bertolotti, R., Parvez, S.H., and Nagatsu, T.). 18 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hanazono, Y.: "Cell Therapy"Springer-Verlag, Tokyo(ed. by Ikeda, Y., Hata, J., Koyasu, S., Kawakami, Y, Hattori, Y.). 11 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hasumi Y., Mizukami H., Urabe M., Kohno T., Takeuchi K., Kume A., Momoeda M., Yoshikawa H., Tsuruo T., Shibuya M., Taketani, Y., and Ozawa K.: "Soluble Flt-1 expression suppresses carcinomatous ascites in nude mice bearing ovarian cancer"Cancer Res.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wang L., Muramatsu S., Lu, Y., Ikeguchi K., Fujimoto K., Okada T., Mizukami H., Hanazono Y., Kume A., Urano F., Ichinose H., Nagatsu T., Nakano I., and Ozawa K.: "Delayed delivery of AAV-GDNF prevents nigral neurodegeneration and promotes functional recovery in a rat model of Parkinson's disease"Gene Ther.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shimpo M., Ikeda U., Maeda Y., Takahashi M., Miyashita H., Mizukami H., Urabe M., Kume A., Takizawa T., Shibuya M., Ozawa K., and Shimada K.: "AAV-mediated VEGF gene transfer into skeletal muscle stimulates angiogenesis and improves blood flow in a rat hindlimb ischemia model"Cardiovasc. Res.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Muramatsu S., Fujimoto K., Ikeguchi K., Shizuma N., Kawasaki K., Ono, F., Shen Y., Wang L., Mizukami H., Kume K., Matsumura M., Nagatsu N., Urano F., Ichinose H., Nagatsu T., Terao, T., Nakano I., and Ozawa K.: "Behavioral recovery in a primate model of Parkinson's disease by triple transduction of striatal cells with adeno-associated viral vectors expressing dopamine-synthesizing enzymes"Hum. Gene Ther.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Okada T., Shimazaki K., Nomoto T., Matsushita T., Mizukami H., Urabe, M, Hanazono Y., Kume A., Tobita K., Ozawa K., and Kawai N.: "Adeno-associated viral vector-mediated gene therapy of ischemia-induced neuronal death"Method. Enzymol.. 346. 378-393 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kawada T., Nakazawa M., Nakauchi S., Yamazaki K., Shimamoto R., Urabe, M., Nakata J., Hemmi C., Masui F., Nakajima T., Suzuki J. I., Monahan J., Sato H., Masaki T., Ozawa K., and Toyo-Oka T.: "Rescue of hereditary form of dilated cardiomyopathy by rAAV -mediated somatic gene therapy : Amelioration of morphological findings, sarcolemmal permeability, cardiac performances, and the prognosis of TO-2 hamsters"Proc. Natl. Acad. Sci. U.S.A.. 99. 901-906 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Okada T., Mizukami H., Urabe M., Nomoto T., Matsushita T., Hanazono Y., Kume A., Tobita K., and Ozawa K.: "Development and characterization of an antisense-mediated regulation system for adeno-associated virus vector production with introduction of Cre recombinase"Biochem. Biophys. Res. Coramun.. 288. 62-68 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Saga Y., Mizukami H., Suzuki M., Urabe M., Kume A., Nakamura T., Sato I., and Ozawa K.: "Expression of HGF/NK4 in ovarian cancer cells suppresses intraperitoneal dissemination and extends host survival"Gene Ther.. 8. 1450-1455 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Muramatsu M., Hanazono Y., Ogasawara Y., Okada T., Mizukami H., Kume A., Mizoguchi H., and Ozawa K.: "Reversible integration of the dominant negative retinoid receptor gene for ex vivo expansion of hematopoietic stem/progenitor cells"Biochem. Biophys. Res. Commun.. 27. 891-896 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maeda Y., Ikeda U., Oya K., Shimpo M., Ueno S., Urabe M., Kume A., Monahan J., Ozawa K., and Shimada K.: "Adeno-associated virus-mediated transfer of endothelial nitric oxide synthase gene inhibits protein synthesis of rat ventricular cardiomyocytes"Cardiovas. Drug Ther.. 15. 19-24 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kogure K., Urabe M., Mizukami H., Kume A., Sato Y., Monahan J., and Ozawa K.: "Targeted integration of foreign DNA into a defined locus on chromosome 19 in K562 cells using AAV-derived components"Int. J. Hematol.. 73. 469-475 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Xin K.-Q., Urabe M., Yang J., Nomiyama K., Mizukami H., Hamajima K., Nomiyama H., Saito T., Imai M., Monahan J., Okuda K., Ozawa K. and Okuda K.: "A novel recombinant adeno-associated virus vaccine induces a long-term humoral immune response to human immunodeficiency virus"Hum. Gene Ther.. 12. 1047-1061 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maeda Y., Ikeda U., Shimpo M., Ishibashi S., Takizawa T., Monahan J., Ozawa K. and Shimada K.: "Adeno-associated virus-mediated transfer of endothelial nitric oxide synthase gene reduces vasoconstrictive response"Exp. Clin. Cardiol.. 6. 50-55 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kawada T., Sakamoto A., Nakazawa M., Urabe M., Masuda F., Hemmi C., Wang Y., Soo Shin W., Nakatsuru Y., Sato H., Ozawa K., and Toyo-oka T.: "Morphological and physiological restorations of hereditary form of dilated cardiomyopathy by somatic gene therapy"Biochem. Biophys. Res. Commun.. 284. 431-435 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanaka M., Kirito K., Kashii Y., Uchida M., Watanabe T., Endo H., Endoh T., Sawada K., Ozawa K. and Komatsu N.: "Forkhead family transcription factor FKHRL1 is expressed in human megakaryocytes and regulates cell cycling as a downstream molecule of TPO signaling"J. Biol. Chem.. 276. 15082-15089 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kanazawa T., Urabe M., Mizukami H., Okada T., Kume A., Nishino H., Monahan J., Kitamura K., Ichimura K., and Ozawa K.: "γ-Rays enhance rAAV-mediated transgene expression and cytocidal effect of AAV-HSVtk/ganciclovir on cancer cells"Cancer Gene Ther.. 8. 99-106 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fan D., Shen Y., Kang D., Nakano I., and Ozawa K.: "Adeno-associated virus vector-mediated triple gene transfer of dopamine synthetic enzymes"Chinese Med. J.. 114. 1276-1279 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shinjyo T., Kuribara R., Inukai T., Hosoi H., Kinoshita T., Miyajima A., Houghton P.J., Look A.T., Ozawa K., and Inaba T.: "Downregulation of Bim, a proapoptotic relative of Bcl-2, is a pivotal step in cytokine-initiated survival signaling in murine hematopoietic progenitors"Mol. Cell. Biol.. 21. 854-864 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ozawa K., Fan, D.-S., Shen Y., Muramatsu S., Fujimoto K., Ikeguchi K., Ogawa M, Urabe M., Kume A., and Nakano I.: "Gene therapy of Parkinson's disease using adeno-associated virus (AAV) vectors"J. Neural Transm. [suppl] 58. 181-191 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maeda Y., Ikeda U., Shimpo M., Shibuya M., Monahan J., Urabe M., Ozawa K., and Shimada K.: "Adeno-associated virus-mediated vascular endothelial growth factor gene transfer into cardiac myocytes"J. Cardiovasc. Pharm.. 36. 438-443 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Urabe M., Shimazaki K., Saga Y., Okada T., Kume A., Tobita K. and Ozawa K.: "Self-amplification system for recombinant adeno-associated virus production"Biochem. Biophys. Res. Commun.. 276. 559-563 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ozawa K., Muramatsu S., Ikeguchi K., Fujimoto K., Shen Y., Fan D.-S., Mizukami H., Urabe M., Hanazono Y., Kume A., Ogawa M, and Nakano I.: "Adeno-associated virus (AAV) vectors and gene therapy of Parkinson's disease"Progress in Gene Therapy-Basic and Clinical Frontiers (ed. by Bertolotti, R., Parvez, S.H., and Nagatsu, T.). 289-306 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shen Y., Muramatsu S., Ikeguchi K., Fujimoto K., Fan D., Ogawa M., Mizukami H., Urabe M., Kume A., Nagatsu I., Urano F., Suzuki T., Ichinose H., Nagatsu T., Monahan J., Nakano I. and Ozawa K.: "Triple transduction with adeno-associated virus vectors expressing tyrosine hydroxylase, aromatic L-amino acid decarboxylase, and GTP cyclohydrolase I for gene therapy of Parkinson's disease"Hum. Gene Ther.. 11. 1509-1519 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shimpo M., Ikeda U., Maeda Y., Ueno S., Ikeda M., Minota S., Takizawa T., Urabe M., Kume A., Monahan J., Ozawa K. and Shimada K.: "Gene transfer into rat renal cells using adeno-associated virus vectors"Am. J. Nephrol.. 20. 242-247 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shimazaki K., Urabe M., Monahan J, Ozawa K., and Kawai N.: "Adeno-associated virus vector-mediated bcl-2 gene transfer into post-ischemic gerbil brain in vivo: prospects for gene therapy of ischemia-induced neuronal death"Gene Ther.. 7. 1244-1249 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kume A., Xu. R., Ueda Y., Urabe M. and Ozawa K.: "Long-term tracking of murine hematopoietic cells transduced with a bicistronic retrovirus containing CD24 and EGFP genes"Gene Ther.. 7. 1193-1199 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hanazono Y., Dunbar C.E., Donahue R.E., Kato I., Ueda Y., Hasegawa M., Urabe M., Kume A., Terao, K. and Ozawa K.: "Basic studies toward hematopoietic stem cell gene therapy"Cell Therapy (ed. by Ikeda, Y., Hata, J., Koyasu, S., Kawakami, Y., Hattori, Y.). 159-169 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsuda K.M., Madoiwa S., Hasumi Y., Kanazawa T., Saga Y., Kume A., Mano H., Ozawa K. and Matsuda M.: "A novel strategy for the tumor angiogenesis-targeted gene therapy: Generation of angiostatin endogenous plasminogen by protease gene transfer"Cancer Gene Ther.. 7. 589-596 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Muroi K., Suzuki T., Amemiya Y., Yoshida M., Kawano C., Kuribara R., Otsuki T., Izumi T., Tomizuka H., Komatsu N., Imagawa S., Hatake K., Miura Y. and Ozawa, K.: "Autologous peripheral blood stem cell transplantation for adults with B-lineage acute lymphoblastic leukemia: a pilot study"Leukemia Lymphoma. 38. 103-111 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Urabe M., Kume A., Tobita K. and Ozawa K.: "DNA/calcium phosphate precipitates mixed with medium are stable and maintain high transduction efficiency"Anal. Biochem.. 278. 91-92 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maeda Y., Ikeda U., Oya K., Shimpo M., Ueno S., Okada K., Saito T., Mano H., Ozawa K. and Shimada K.: "Endogenously generated nitric oxide by nitric-oxide synthase gene transfer inhibits cellular proliferation"J. Phamacol. Exp. Therapeut.. 292. 387-393 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hasumi, Y.: "Soluble Flt-1 expression suppresses carcinomatous ascites in nude mice bearing ovarian cancer"Cancer Res.. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Wang, L.: "Delayed delivery of AAV-GDNF prevents nigral neurodegeneration and promotes functional recovery in a rat model of Parkinson's disease"Gene Ther.. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Shimpo, M.: "AAV-mediated VEGF gene transfer into skeletal muscle stimulates angiogenesis and improves blood flow in a rat hindlimb ischemia model"Cardiovasc. Res.. (in press).

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  • [Publications] Muramatsu, S.: "Behavioral recovery in a primate model of Parkinson's disease by triple transduction of striatal cells with adeno-associated viral vectors expressing dopamine-synthesizing enzymes"Hum.Gene Ther.. (in press).

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  • [Publications] Okada, T.: "Adeno-associated viral vector-mediated gene therapy of ischemia-induced neuronal death"Method.Enzymol.. 346. 378-393 (2002)

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  • [Publications] Kawada, T.: "Rescue of hereditary form dilated cardiomyopathy by rAAV-mediated somaticgene therapy : Amelioration of morphological findings, sarcolemmal permeability, cardiac performances, and the prognosis of TO-2 hamsters"Proc.Natl.Acad.Sci.U.S.A.. 99. 901-906 (2002)

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  • [Publications] Okada, T.: "Development and characterization of an antisense-mediated regulation system for adeno-associated virus vector production with introduction of Cre recombinase"Biochem.Biophys.Res.Commun.. 288. 62-68 (2001)

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  • [Publications] Saga, Y.: "Expression of HGF/NK4 in ovarian cancer cells suppresses intraperitoneal dissemination and extends host survival"Gene Ther.. 8. 1450-1455 (2001)

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  • [Publications] Muramatsu, M.: "Reversible integration of the dominant negative retinoid receptor gene for ex vivo expansion of hematopoietic stem/progenitor cells"Biochem.Biophys.Res.Commun.. 27. 891-896 (2001)

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  • [Publications] Maeda, Y.: "Adeno-associated virus-mediated transfer of endothelial nitric oxide synthase gene inhibits protein synthesis of rat ventricular cardiomyocytes"Cardiovas. Drug Ther.. 15. 19-24 (2001)

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  • [Publications] Kogure, K.: "Targeted integration of foreign DNA into a defined locus on chromosome 19 in K562 cells using AAV-derived components"Int.J.Hematol.. 73. 469-475 (2001)

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  • [Publications] Xin, K.-Q.: "A novel recombinant adeno-associated virus vaccine induces a long-term humoral immune response to human immunodeficiency virus"Hum.Gene Ther.. 12. 1047-1061 (2001)

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  • [Publications] Maeda, Y.: "Adeno-associated virus-mediated transfer of endothelial nitric oxide synthase gene reduces vasoconstrictive response"Exp.Clin.Cardiol.. 6. 50-55 (2001)

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  • [Publications] Kawada, T.: "Morphological and physiological restorations of hereditary form of dilated cardiomyopathy by somatic gene therapy"Biochem.Biophys.Res.Commun.. 284. 431-435 (2001)

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  • [Publications] Kanazawa, T.: "γ-Rays enhance rAAV-mediated transgene expression and cytocidal effect of AAV-HSVtk/ganciclovir on cancer cells"Cancer Gene Ther.. 8. 99-106 (2001)

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  • [Publications] Fan, D.: "Adeno-associated virus vector-mediated triple gene transfer of dopamine synthetic enzymes"Chin.Med.J.. 114. 1276-1279 (2001)

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  • [Publications] Kanazawa,T.: "Gamma rays enhance rAAV-mediated transgene expression and cytocidal effect of AAV-HSVtk/ganciclovir on cancer cells."Cancer Gene Ther.. (in press).

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  • [Publications] Shinjyo,T.: "Downregulation of Bim,a proapoptotic relative of Bcl-2, is a pivotal step in cytokine-inititated survival signaling in murine hematopoietic progenitors."Mol.Cell.Biol.. 21. 854-864 (2001)

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  • [Publications] Ozawa,K.: "Gene therapy of Parkinson's disease using adeno-associated virus (AAV) vectors."J.Neural Transm. [suppl]. 58. 181-191 (2000)

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  • [Publications] Maeda,Y.: "Adeno-associated virus-mediated vascular endothelial growth factor gene transfer into cardiac myocytes."J.Cardiovasc.Pharm.. 36. 438-443 (2000)

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  • [Publications] Urabe,M. : "Self amplification system for recombinant adeno-associated virus production."Biochem.Biophys.Res.Commun.. 276. 559-563 (2000)

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  • [Publications] Shen,Y.: "Triple transduction with adeno-associated virus vectors expressing tyrosine hydroxylase,aromatic I-amino acid decarboxylase, and GTP cyclohydrolase 1 for gene therapy of Parkinson's disease."Hum.Gene Ther.. 11. 1509-1519 (2000)

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  • [Publications] Shimpo,M.: "Gene transfer into rat renal cells using adeno-associated virus vectors."Am.J.Nephrol.. 20. 242-247 (2000)

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  • [Publications] Shimazaki,K.: "Adeno-associated virus vector-mediated bcl-2 gene transfer into post-ischemic gerbil brain in vivo : prospects for gene therapy of ischemia-induced neuronal death."Gene Ther.. 7. 1244-1249 (2000)

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  • [Publications] Kume,A.: "Long-term tracking of murine hematopoietic cells transduced with a bicistronic retrovirus containign CD24 and EGFP genes."Gene Ther.. 7. 1193-1199 (2000)

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  • [Publications] Matsuda,K.M.: "A novel strategy for the tumor angiogenesis-targeted gene therapy : Generation of angiostatin endogenous plasminogen by protease gene transfer."Cancer Gene Ther.. 7. 589-596 (2000)

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  • [Publications] Muroi,K.: "Autologous periphcral blood stem cell transplantation for adults with B-lineage acute lymphoblastic leukemia : a pilot study."Leukemia Lumphoma. 38. 103-111 (2000)

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  • [Publications] Urabe,M.: "DNA/calcium phosphate precipitates mixed with medium are stable and maintain high transduction efficiency."Anal.Biochem.. 278. 91-92 (2000)

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  • [Publications] Maeda,Y.: "Endogenously generated nitric oxide by nitric-oxide synthase gene transfer inhibits cellular proliferation."J.Phamacol.Exp.Therapeut.. 292. 387-393 (2000)

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  • [Publications] Ozawa,K.: "Progress in Gene Therapy - Basic and Clinical Frontiers."VSP BV, Netherlands (ed. by Bertolotti,R.,Parvez,S.H.,and Nagatsu,T.) . 18 (2000)

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  • [Publications] Hanazono,Y.: "Cell Therapy"Springer-Verlag,Tokyo (ed. by Ikeda,Y.,Hata,J.,Koyasu,S.,Kawakami,Y.,Hattori,Y.). 11 (2000)

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Published: 2000-04-01   Modified: 2016-04-21  

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