HOMEOTIC TRANSCRIPTION FACTORS THAT BIND TO FOLLICLE-STIMULATING HORMONE SUBUNITE GENE

• Project/Area Number: 12470217
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Endocrinology
• Research Institution: Meiji University
• Principal Investigator: KATO Yukio MEIJI UNIVERSITY, SCHOOL OF AGLICULTURE, PROFESSOR, 農学部, 教授 (30114177)
• Project Period (FY): 2000 – 2002
• Project Status: Completed (Fiscal Year 2002)
• Budget Amount: ¥14,200,000 (Direct Cost: ¥14,200,000); Fiscal Year 2002: ¥2,700,000 (Direct Cost: ¥2,700,000); Fiscal Year 2001: ¥4,000,000 (Direct Cost: ¥4,000,000); Fiscal Year 2000: ¥7,500,000 (Direct Cost: ¥7,500,000)
• Keywords: PITUITARY / RECOMBINANT DNA / GONADOTROPIN / CLONING / TRANSCRIPTION FACTOR / GENE / HOMEOBOX / YEST ONE HYBRID / 生殖腺刺激ホルモン / 遺伝子

Research Abstract

(1) FOUR TRANSCRIPTION FACTORS THAT BIND TO THE UPSTREAM REGION OF PORCINE FOLLICLE STIMULATING HORMONE SUBUNIT GENE HAVE BEEN CLONED USING YEAST ONE-HYBRID SYSTEM.
(2) THE NUCEOTIDE SEQUENCES OF CLONES HAVE HOMEOTIC REGION, WHICH RECOGNIZES SPECIFIC DNA SEQUENCES.
(3) PRODUCTION OF RECOMBINANT TRANSCRIPTION FACTOR HAVE BEEN SUCCEEDED AND THE GEL MOBILITY SHIFT ASSAY DEMONSTRATED SPECIFIC BINDING FOR FSH β GENE
(4) EXPRESSION VECTORS CONTAINING CLONED TRANSCRIPTION FACTORS WERE TRANSFECTED IN THE CHO CELLS TOGETHER WITH A REPORTER VECTOR FUSED WITH THE 5'-UPSTREAM REGION OF THE FSHβ GENE AND SOME STIMULATION OF THE GENE EXPRESSIO WAS OBSERVED.

Research Products

• [Publications] Chen, L.: "Cytological characterization of a pituitary folliclo-stellate-like cell line, Tpit/F1, with special reference to adenosine triphosphate-mediated neural nitric oxide synthetase expression and nitric oxide secretion"Endocrinology. 141. 3603-3610 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nishida, N.: "Critical role of nitric oxide in expression of porcine LH receptor at transcription and post-transcription levels"Experimental and Clinical Endocrinology and Diabetis. 108. 424-429 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nishida, N.: "Transcription factor genes, c-fos and c-jun, in developing porcine granulosa cells in vitro"Journal of Reproduction and Development. 46. 167-175 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hattori M.: "FSH suppression of nitric oxide synthesis in porcine oocytes"Journal of Molecular Endocrinology. 24. 65-73 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 加藤幸雄: "成長ホルモン遺伝子とプロラクチン遺伝子の発現スイッチングのリアルタイム測定系の開発"成科学協会研究年報. 23. 167-172 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Takesue, K.: "Stimulation of nitric oxide synthetase in porcine granulosa cells by phorbol ester"Journal of Reproduction and Development. 47. 1-6 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hattori MA: "Retinoic acid suppression of endothelial nitric oxide synthase in porcine oocyte"Can J Physiol Pharmacol. 80. 777-782 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hattori, M-A.: "Preferential expression of ADP-ribosylation factor gene in the chick embryonic gonads"Life Science. 70. 2031-2037 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hattori, M-A.: "Prominent expression of transforming growth factor β2 gene in the chicken embryonic gonad as revealed by suppressive subtraction cloning"Gen Comp Endocrinol 2002;125:311-316. 125. 311-316 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Iwasawa A: "Changes in thyrotropin messenger RNA and circulating hormone levels during induced molting of the hen (Gallus domesticus)"J Reprod Dev 2002;48:489-496. 48. 489-496 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Iwasawa A: "Specific anti-peptide antibody to β subunit of chicken thyrotropin : production and characterization"Endocr J. 48. 197-204 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tomizawa K: "Production and Characterization of an Antiserum against Recombinant Porcine Follicle Stimulating Hormone"J Reprod Dev 2002;48:131-136. 48. 131-136 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nishida, N.: "Expression of endothelial nitric oxide synthase gene in cultured porcine granulosa cells after FSH stimulation"Jounal of Molecular Endocrinology. 26. 259-265 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hattori, M-A.: "Expression of endothelial nitric oxide synthetase in the porcine oocyte and its possible function"Molecular and Cellular Biology. 219. 121-126 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hattori, M-A.: "Synthesis of nitric oxide in the porcine ovary and its possible function"Reproductive Biotechnology. 99-103 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kato, Y.: "Presence of an 85 kDa pituitary protein (Pit-G) in porcine GH-producing cells"Journa of Reproduction and Development. 48. 87-95 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 本比較内分泌学会編: "講談社"ブルーバックス「生命をあやつるホルモン」(共著). 20 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Chen L, Maniyama D, Sugiyama M, Sakai T, Mogi C, Kato M, Kurotani R, Shirasawa N, Takaki A, Renner U, Kato Y and Inoue K: "Cytological characterization of a pituitary folliclo-stellate-like cell line, Tpit/F1, with special reference to adenosine triphosphate-mediated neural nitric oxide synthetase expression and nitric oxide secretrion."Endocrinology. 141. 3603-3310 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hattori M-A, Nishida N, Takesue K, Kato Y and Fujihara N.: "Follicle-stimulating hormone suppression of nitric oxide synthesis in the porcine oocytes"J Mol Endocrinol. 24. 65-73 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nishida N, Takesue K, Hattori M-A, Kato Y and Fujihara N.: "Critical role of nitric oxide in expression of porcine LH receptor at transcription and post-transcription levels."Exp Clin Endocrinol Diabetis. 108. 424-429 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nishida N, Takesue K, Hattori M-A, Kato Y, Wakabayashi K and Fujihara N.: "Modulatory action of nitric oxide on the expression of transcription factor genes, c-fos and c-jun, in developing porcine granulosa cells."J Reprod Dev. 46. 167-175 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shimizu H, Ohtani K, Kato Y and Mori M.: "Interleukin-6 increases insulin secretion and preproinsulin mRNA expression via Ca2+-dependent mechanism"J Endocrinol. 166. 121-126 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hattori M-A, Takesue K, Kato Y and Fujihara N.: "Expression of endothelial nitric oxide synthetase in the porcine oocyte and its possible function."Mol Cell Biochem. 219. 121-126 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Takesue K, Nishida N, Hattori M-A, Kato Y and Fujihara N.: "Stimulation of nitric oxide synthetase in porcine granulosa cells by phorbol ester."J Reprod Dev. 47. 1-6 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hattori MA, Kato Y and Fujihara N.: "Retinoic acid suppression of endothelial nitric oxide synfliase in porcine oocyte."Can J Physiol Pharmacol. 80. 777-782 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hattori M-A, Fujioka T, Kato Y and Fujiwara N.: "Preferential expression of ADP-ribosylation factor gene in the chick embryonic gonads."LF. 70. 2031-2037 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hattori M-A, Furuta H, Hiyama Y, Kato Y and Fujihara N.: "Prominent expression of transforming growth factor b2 gene in the chicken embryonic gonad as revealed by suppressive subtraction cloning."Gen Comp Endocrinol. 125. 311-316 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Iwasawa A, Kato Y, Hishikawa T, Doi O, Kamiyoshi M and Nakamura T.: "Changes in thyrotropin messenger RNA and circulating hormone levels during induced molting of the hen (Gallus domesticus)."J Reprod Dev. 48. 489-496 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Iwasawa A, Nakayama H, Yamamoto Y, Doi O, Nakamura T and Kato Y.: "Specific anti-peptide antibody to b subunit of chicken throtoropin: production and characterization."Entdocr J. 48. 197-204 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kato Y, Kato T, Tomizawa K, Ogawa S, Hattori M-A and Inoue K.: "Presence of an 85 kDa pituitary protein (Pit-G) in porcine GH-producing cells."J Reprod Dev. 48. 87-95 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tomizawa K, Ogawa S, Kato T, Watanabe M, Sato I, Sakai T, Hattori M-A, Inoue K, Ueda S, Kikuchi M, Mori J and Kato Y.: "Production and Characterization of an Antiserum against Recombinant Porcine Follicle Stimulating Hormone"J Reprod Dev. 48. 131-136 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Aikawa S, Kato T and Kato Y.: "Molecular Cloning of Porcine Neuronatin and Analysis of Its Exprssion during Pituitary Ontogeny."Exp. Clinic. Endocrinol. Diabetes. in press.
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hattori M-A, Nishida N, Takesue K, Kato Y and Fujihara N.: "Synthesis of nitric oxide in the porcine ovary and its possible function., Reproductive Biotechnology, ed. Miyamoto H N Manabe."Hokuto Printing Company. 99-103 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hattori MA: "Retinoic acid suppression of endothelial nitric oxide synthase in porcine oocyte"Can J Physiol Pharmacol. 80. 777-782 (2002)
• [Publications] Hattori, M-A.: "Preferential expression of ADP-ribosylation factor gene in the chick embryonic gonads"Life Science. 70. 2031-2037 (2002)
• [Publications] Hattori, M-A.: "Prominent expression of transforming growth factor β2 gene in the chicken embryonic gonad as revealed by suppressive subtraction clonin"Gen Camp Endocrinol 2002 ; 125 : 311-316. 125. 311-316 (2002)
• [Publications] Iwasa A: "Changes in thyrotropin messenger RNA and circulating hormone levels during induced molting of the hen (Gallus domesticus)"J Reprod Dev 2002 ; 48: 489-496. 48. 489-496 (2002)
• [Publications] Iwasa A: "Specific anti-peptide antibody to β subunit of chicken thyrotropin : production and characterization"Endocr J. 48. 197-204 (2002)
• [Publications] Tomizawa K: "Production and Characterization of an Antiserum against Recombinant Porcine Follicle Stimulating Hormone"J Reprod Dev 2002 ; 48: 131-136. 48. 131-136 (2002)
• [Publications] Soh T: "Utilization of central disk of blastoderm and germinal crescent region for production of interspecific germline chimera between chicken and quail"Asian J Andorol 2002 ; 4 : 83-6. 4. 83-86 (2002)
• [Publications] Kato Y: "Presence of an 85 kDa pituitary protein (Pit-G) in porcine GH-producing cells"J Reprod Dev 2002 ; 48 : 87-95. 48. 87-95 (2002)
• [Publications] Aikawa S: "Molecular Cloning of Porcine Neuronatin and Analysis of Its Expression during Pituitary Ontogeny"Endocrinol. Diabetes. (印刷中).
• [Publications] 本比較内分泌学会編: "ブルーバックス「生命をあやつるホルモン」(共著)"講談社. 20 (2003)
• [Publications] Nishida, N.: "Expression of endothelial nitric oxide synthase gene in cultured porcine granulosa cells after FSFTJ stimulation"Journal of Molecular Endocrinology. 26. 259-265 (2001)
• [Publications] Hattori, M-A.: "Expression of endothelial nitric oxide synthetase in the porcine oocyte and its possible function"Molecular and Cellular Biology. 219. 121-126 (2001)
• [Publications] Hattori, M-A.: "Synthesis of nitric oxide in the porcine ovary and its possible function"Reproductive Biotechnology. 1. 99-103 (2001)
• [Publications] Kato, Y.: "Presence of an 85 kDa pituitary protein (Pit-G) in porcine GH-producing cells"Journal of Reproduction and Development. 48. 87-95 (2002)
• [Publications] Chen,L.: "Cytological characterization of a pituitary folliclo-stellate-like cell line. Tpit/F1, with special reference to adenosine triphosphate-mediated neuronal nitric oxide synthase expression and nitric oxide secretion"Endocrinology. 141. 3603-3610 (2000)
• [Publications] Nishida,N.: "Critical role of nitric oxide in expression of porcine LH receptor at transcription and post-transcription levels"Experimental and Clinical Endocrinology and Diabetes. 108. 424-429 (2000)
• [Publications] Nishida,N.: "Transcription facgtor genes, c-fos and c-jun, in developing porcine granulosa cells in vitro"Journa of Reproduction and Development. 46. 167-175 (2000)
• [Publications] Hattori M.: "FSH suppression of nitric oxide synthesis in porcine oocytes"Journa of Molecular Endocrinology. 24. 65-73 (2000)
• [Publications] 加藤幸雄: "成長ホルモン遺伝子とプロラクチン遺伝子の発現スイッチングのリアルタイム測定系の開発"成科学協会研究年報. 23. 167-172 (2000)
• [Publications] Takesue,K.: "Stimulation of nitric oxide synthesis in porcine granulosa cells by phorbol ester"Journa of Reproduction and Development. 47. 1-6 (2001)