| Project/Area Number |
12470235
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Chiba University |
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Principal Investigator |
KENMOCHI Takashi Chiba University, University Hospital, Lecturer, 医学部・附属病院, 講師 (50215133)
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| Co-Investigator(Kenkyū-buntansha) |
SAIGO Kenichi Chiba University, University Hospital, Assistant, 医学部・附属病院, 助手 (60323424)
WADA Yuichi Chiba University, University Hospital, Assistant, 医学部・附属病院, 助手 (10282485)
ASANO Takehide Chiba University, Graduate School of Medicine, Lecturer, 大学院・医学研究院, 講師 (80143311)
AKUTU Naotake Chiba University, University Hospital, Medical Stuff, 医学部・附属病院, 医員
MARUYAMA Michihiro Chiba University, University Hospital, Medical Stuff, 医学部・附属病院, 医員
丸山 通広 千葉大学, 医学部・附属病院, 医員
宮内 英聡 千葉大学, 医学部・附属病院, 医員
松井 芳文 千葉大学, 医学部・附属病院, 医員
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥11,700,000 (Direct Cost: ¥11,700,000)
Fiscal Year 2001: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 2000: ¥6,300,000 (Direct Cost: ¥6,300,000)
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| Keywords | islet transplantation / islet isolation / Chondrocytes / Gene transfer / hydroxyethyl starch / 凍結保存 / 遺伝子銃 |
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| Research Abstract |
Experiment 1. Effectiveness of hydroxyethyl starch (HES) on purification of pancreatic islets. The yield, purity, and viability of islets isolated from Beagle pancreata were significantly improved by the use of HES-Collins solution as compared to widely used Euro-Ficoll solution. Islet purification using HES-Collins solution might be recommended for clinical islet transplantation. Experiment 2. Co-culture of rat islets with allogenic chondrocvtes. Islets isolated from DA rat were co-cultured with the chondrocytes isolated from Lewis rat. The islets were surrounded with chondrocytes at 10 days culture and insulin release into media was maintained. Chondrocyte was suggested to become a new material for Bio matrix islet graft. Experiment 3. Gene transfer into canine islets. PCMS-EGFP was transferred into the islats from Beagle pancreata using 1) Gene gun, 2) Electroporarion, and 3) Lipofection. Lipofection technique was simple and less harmful for the isles. Moreover, insulin release was well preserved with Lipofection technique.
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