| Project/Area Number |
12470255
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
ANDO Hisami Graduate School of Medicine, Nagoya University Professor, 大学院・医学研究科, 教授 (60184321)
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| Co-Investigator(Kenkyū-buntansha) |
MARUI Yuji University Hospital, Nagoya University Medical Staff, 医学部・附属病院, 医員
KANEKO Kenichirou University Hospital, Nagoya University Assistant Professor, 医学部・附属病院, 助手 (90335042)
WATANABE Yoshio Graduate School of Medicine, Nagoya University Associate Professor, 大学院・医学研究科, 助教授 (80201242)
KATSUNO Shinsuke University Hospital, Nagoya University Medical Staff, 医学部・附属病院, 医員 (90324409)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥9,900,000 (Direct Cost: ¥9,900,000)
Fiscal Year 2001: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥6,400,000 (Direct Cost: ¥6,400,000)
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| Keywords | choledochal cyst / pancreaticobiliary malfunction / bile duct / congenital anomaly / 神経ネットワーク / 免疫組織染色 / hypoganglionosis / ヒルミュミュプルング病 |
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| Research Abstract |
1. This study analyzed the bile duct walls of patients with choledochal cyst as to whether the nerve fibers and nerve cells in the wall are abnormal or not. Bile ducts obtained from eight patients with choledochal cyst and three normal bile ducts without dilatation were studied Whole-mount preparations displaying the myenteric plexus running beneath the outer surface of the bile duct were made by removing the mucosa, submucosa, fibromuscular layers under a stereomicroscope. Whole-mount preparations were immersed in 0.3% Triton X-phosphate buffer solution for 2 to 3 days. They were immunohistochemically stained using the streptavidin-biotin-peroxidase method after 6- to 7-day incubation with anti-protein gene product (PGP) 9.5 antibody as the primary antibody.
2. Developed networks of the myenteric plexus were observed in the fibromuscular layer near the outer surface. Distribution of the ganglia was clearly observed. Nerve strands of the patients were not significantly different from those of controls, but the number of ganglia of the patients decreased to one tenth of that of controls.
3. This distribution of nerve strands and ganglia in the bile duct wall of choledochal cyst is similar to that in the intestinal wall of hypoganglionosis, which implies the mechanism of dilatation of the bile duct in patients with choledochal cyst is the same as that of megacolon in patients with hypoganglionosis.
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