• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Establishment of the method for hepatocytes differentiation from embryonic stem cells in vitro

Research Project

Project/Area Number 12470256
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKyoto University

Principal Investigator

HIROSE Tetsuro  Institute for Frontier Medical Sciences, Instructor, 再生医科学研究所, 助手 (00314279)

Co-Investigator(Kenkyū-buntansha) NAKATSUJI Norio  Institute for Frontier Medical Sciences, Professor, 再生医科学研究所, 教授 (80237312)
YAMAOKA Yoshio  Graduate School of Medicine., Professor, 医学研究科, 教授 (90089102)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2001: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2000: ¥10,900,000 (Direct Cost: ¥10,900,000)
KeywordsEmbryonic Stem Cells / Hepatic Progenitor Cells / Endoderm / Transgenic Mice / Hepatocyte Growth Factor / Bone Marrow Cells / 肝細胞 / 分化誘導 / 蛍光励起セルソーター / 内胚葉レポーティングES細胞 / Cre / loxP / アルブミン / HGF
Research Abstract

We have established a detection system for hepatocytes induced from embryonic stem cells using a reporter gene construct that includes Green Fluorescent Protein (GFP) regulated under the enhancer/ promoter of the albumin gene, one of the endodermal markers present during the course of development. We have modified the reporter gene construct using the insulator sequence and the Cre-/loxP system because the albumin enhancer/promoter has weak activity, and the expression of albumin gene is affected by the promoter of the drug resistant gene included in the construct. Furthermore, we are now going to verify the efficacy of this system through the investigation of transgenic mice which have been produced with this reporter gene construct. In addition, we intend to use immature and mature hepatocytes induced from embryonic *m cells for cell therapy treatment of liver diseases. For this purpose, we have also investigated the role of growth factors and hepatic progenitor/stem cells on liver r … More egeneration. Consequently, we discovered that hepatocyte growth factor (HGF) attenuates the hepatic ischemia-reperfusion injury due to the reduction of oxidative stress (Oe et al., J. Hepatology 2001). Furthermore, we determined that bone marrow cells differentiate into vascular endothelial cells and Kupper cells in the regenerative liver after partial hepatectomy, while not into hepatocytes as previously reported in the animal model of fluminant hepatitis (Fujii et al., J. Hepatology 2002). We also established an effective isolation method and a highly efficient gene transfer system for mouse fetal hepatic progenitor cells (Yasuchika et al., Hepatology 2002). We intend to investigate the characteristics of hepatic progenitor/stem cells and establish a maturation system for these cells using the reporter gene construct as previously described. We can isolate hepatic progenitor cells from adult liver tissue using the same method as from fetal liver tissue (Azuma et al., Hepatology 2003), and are now going to investigate the characteristics of these cells. Less

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Oe S, Hirose T, et al.: "Continous intravenous infusion of hepatocyte growth factor attenuates hepatic ischemia-reperfusion injury in rats"Journal of Hepatology. 34(6). 832-839 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujii H, Hirose T, et al.: "Contribution of bone marrow cells to liver regeneration after partial hepatectomy in mice"Journal of Hepatology. 36(5). 653-659 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasuchika K, Hirose T, et al.: "Establishmen of a highly efficient gene transfer system for mouse fetal hepatic progenitor cells"Hepatology. 36(6). 1488-1497 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Azuma H, Hirose T, et al.: "Hepatic progenitor cells from adult mouse liver"Hepatology. (in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Oe S, Hirose T: "Continous intravenous infusion of hepatocyte growth factor attenuates hepatic ischemia reperiusion injury in rats"Journai of Hepatology. 34(6). 832-839 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujii H, Hirose T: "Contribution of bone marrow cells to liver regeneration after partial hepatectomy in mice"Journai of Hepatology. 36(5). 653-659 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasuchika K, Hirose T: "Establishmen of a highly efficient gene transfer system for mouse fetal hepatic progenitor cells"Hepatology. 36(6). 1488-1497 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Azuma H, Hirose T: "Hepatic progenitor cells from adult mouse liver"Hepatology. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Azuma H., Hirose T.et al.: "Enrichment of Alpha-Fetoprotein Positive Immature Endodermal Cells from Adult Mouse Liver"American Journal of Pathology. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Yasuchika K., Hirose T.et al.: "Establishment of a Highly Efficient Gene transfer System For Mouse Fetal Hepatic Progenitor Cells"Hepatology. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Fujii H., Hirose T.et al.: "Contribution of Bone Marrow Cells to Liver regeneration after Partial Hepatectomy"Jurnal of Hepatology. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Oe S., Hirose T.et al.: "Continuous intravenous Infusion of Deleted Form of Hepatocyte Growth Factor Attenuates Ischemia-Reperfusion Injury"J.Hepatology. 34. 832-839 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 廣瀬 哲朗, 山岡 義生: "再生医療の実際とこれからの展望"実験医学. (印刷中).

    • Related Report
      2001 Annual Research Report
  • [Publications] 廣瀬 哲朗, 安近 健太郎: "肝幹細胞"肝胆膵. 42(2). 179-187 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 廣瀬 哲朗, 山岡 義生et al.: "幹細胞・クローン研究プロトコール"ヒト胎児肝細胞の分離と増殖. 6 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Oe S, Hirose T, et al.: "Continuous intravenous infusion of deleted form of hepatocyte growth factor attenuates hepatic ischemia-reperfusion injury in rats"Journal of Hepatology. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 廣瀬哲朗, 安近健太郎: "肝幹細胞"肝胆膵. 42(2). 179-187 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 廣瀬哲朗, 中辻憲夫: "ES細胞のtissue engineeringへの応用"細胞. 32. 338-342 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 廣瀬哲朗, 中辻憲夫: "幹細胞研究概説(ESから分化した細胞を使った細胞移植を中心に)"カレントテラピー. 18. 78-84 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 廣瀬 哲朗: "肝細胞・クーロン研究プロトコール"羊土社. (2001)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2000-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi