| Project/Area Number |
12470269
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Tohoku University |
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Principal Investigator |
KONDO Takashi Tohoku University, Institute of Development, Aging and Cancer, Professor, 加齢医学研究所, 教授 (10195901)
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Yoshinori Tohoku University, Institute of Development, Aging and Cancer, Assistant Professor, 加齢医学研究所, 助手 (90323104)
SATO Masami Tohoku University, Institute of Development, Aging and Cancer, Assistant Professor, 加齢医学研究所, 助教授 (30250830)
MATSUMURA Yuji Tohoku University Hospital, Assistant Professor, 医学部・附属病院, 助手 (80281997)
佐川 元保 東北大学, 医学部・附属病院, 助手 (70292274)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥9,500,000 (Direct Cost: ¥9,500,000)
Fiscal Year 2001: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2000: ¥5,700,000 (Direct Cost: ¥5,700,000)
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| Keywords | FC receptors / knockout mice / skin transplantation / heterotonic tracheal transplatation / allograft rejection / macrophages |
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| Research Abstract |
To clarify the role of Fc receptors in the process of allograft rejection, allotransplantations were performed in FcR γ knockout mice. Skin transplantation was performed between a MHC incompatible combination of mouse (BALB/c→C57BL/6). Recipient C57BL/6 mice were those deficient in FcR γ or wild-type controls. Skin allografts in FcR γ knockout mice and the controls were both rejected within day 7 or 8 post-transplant and there was no difference in the duration of graft survival between the groups. Next, to investigate the role of macrophages that express Fc receptors on cell surface in allograft rejection, heteropic tracheal transplantation was performed in a MHC incompatible combination of rat. Recipient rats were injected with GdCl3, which has an ability to deplete macrophages, on day 0, 7, 14 and tracheal allografts were harvested on day 21. The severity of obstructive airway disease, which is known to develop in association with allograft rejection, was significantly reduced in GdCl3-treated animals compared to controls. This indicates that macrophages play a role in the process of allograft rejection and the development of obstructive airway disease in a model of heterotopic tracheal transplantation in rats.
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