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DEVELOPMENT OF ARTIFICIAL TRACHEA USING CULTURED CELL SHEET

Research Project

Project/Area Number 12470278
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionSt. MARIANNA UNIVERSITY SCHOOL OF MEDICINE

Principal Investigator

OSADA Hiroaki  St. MARIANNA UNIVERSITY SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (90121178)

Co-Investigator(Kenkyū-buntansha) TSUKADA Hisashi  St. MARIANNA UNIVERSITY SCHOOL OF MEDICINE, ASSISTANT, 医学部, 助手 (60308476)
INOUE Hajime  St. MARIANNA UNIVERSITY SCHOOL OF MEDICINE, ASSISTANT PROFESSOR, 医学部, 講師 (60193603)
YOKOTE Kumio  St. MARIANNA UNIVERSITY SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 医学部, 助教授 (40174859)
小島 宏司  聖マリアンナ医科大学, 医学部, 助手 (40288155)
平 泰彦  聖マリアンナ医科大学, 医学部, 助教授 (00154724)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥8,400,000 (Direct Cost: ¥8,400,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2000: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsARTIFICIAL TRACHEA / TRACHEAL RECONSTRUCTION / REGENERATION TRACHEA / 組織工学 / ハイブリット人工気管 / 人工臓器
Research Abstract

Objective : This study was designed to evaluate how various sizes and densities of pores in Dacron tubing may enhance its utility as a tracheal prosthesis.
Methods : Straight vascular prosthesis made of knitted external velour polyester (MICRON) was prepared for pore formation by laser ablation. Two series of experiments were performed to evaluate porosity and porous grafts reinforced with a steel stent. The primary series compared different pore sizes (300μm, 500μm and 700μm) and pore densities (25/cm^2 or 100/cm^2). Grafts were reinforced with an externally heat-sealed silicone ring stent. The second series tested grafts with a pore density of 64/cm^2 and pore size of 500 μm internally reinforced with a stainless steal spiral stent. In all experiments, a 10-cartilage-ring-length of canine mediastinal trachea was resected and prosthesis was then implanted with omantal flap.
Result : Lower pore sizes and densities (300 μm, 25 pores/cm^2) led to essentially no tissue ingrowth. The larger sized pores(700 μm) and density(25 pores/cm^2) led to rapid but excessive ingrowth of granulation tissue. Mid-range pore size (500 μm) and density(100 pores/cm^2) invited steady tissue ingrowth of the entire graft but eventually developed marked luminal stenosis. Stent reinforced prosthesies harboring 500 μm pores at 64 pores/cm^2 maintained an average patency rate of 60% or more (range : 100% to 20%) at least 12 months after implantation.
Conclusion : Our data shows that porosity can enhance the ability for tissue to grow into these tracheal prosthesies. The optimal pore size and density has been found to be approximately 500 μm and 64 pores/cm^2 respectively. Reinforcement of these grafts has also worked well up to 24 months after surgery. This artificial trachea model has led to long-term survivors and seems promising as a basic model for clinical tracheal repair.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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