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Functional recovery of the cerebral infarction following transplantation of the genetically-engineering human neural stem cells

Research Project

Project/Area Number 12470295
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionSapporo Medical University

Principal Investigator

HONMOU Osamu (2001)  Sapporo Medical University School of medicine, Department of Neurosurgery, Assistant Professor, 医学部, 講師 (90285007)

端 和夫 (2000)  札幌医科大学, 医学部, 教授 (30047039)

Co-Investigator(Kenkyū-buntansha) UEDE Teiji  Sapporo Medical University School of Medicine, Department of Neurosurgery, Associate Professor, 医学部, 助教授 (90160184)
本望 修  札幌医科大学, 医学部, 講師 (90285007)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥16,200,000 (Direct Cost: ¥16,200,000)
Fiscal Year 2001: ¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 2000: ¥8,700,000 (Direct Cost: ¥8,700,000)
Keywordsadult / gene / human / infarction / neural stem cell / stroke / transplantation
Research Abstract

Introduction: Although it has generally been assumed that the adult brain is incapable of significant self-repair because of a lack of neurogenesis in the adult mammalian central nervous system (CNS), several studies have reported that the adult mammalian brain harbors neural stem cells that retain the potential for both neural production and differentiation in experimental animal models. These findings offer the prospect of the presence of neural precursors in the adult human brain. The objective of this study was to identify neural stem cells in the adult human CNS and study whether they repair the ischemic brain.
Methdos: Clonal neural stem cells derived from adult human brain were established in vitro. They were genetically marked with LacZ gene and was transplanted into the ischemic brain of the experimental animal. It was investigated if neural tissue reconstruction occurred following transplantation.
Results: Single-cell clonal analysis demonstrated self-renewing and multipotential properties of neural stem cells derived from the adult human brain in vitro. Histological examination of the ischemic lesion following transplantation revealed that the transplanted human cells reconstructed the neural tissue. The transplanted stem cell in the contra-lateral hemisphere also migrated into the ischemic lesion of the another hemisphere, and repaired the damaged brain following ischemic stress. The electrophysiological and behavior studies also proved the functional recovery of the ischemic model animals following the human neural stem cell transplantation.
Conclusion: Adult human brain harbors neural stem cells that retain the potential for both neural production and differentiation. Transplantation of clonal neural stem cells derived from adult human brain repaired the ischemic neural tissue in the mammalian adult CNS.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Honmou, O. et al.: "A cell therapy for the CNS diseases"Restorative Neurology and Neuroscience. (In press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takahashi, A. et al.: "Transplantation of human neural stem cells into the ischemic lesion of the rat brain"Restorative Neurology and Neuroscience. (In press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 本望 修: "神経幹細胞応用の可能性について(総説)"脳神経外科. 29(4). 293-304 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Honmou, O. et al.: "Clonal neural stem cells derived from adult human brain : Implications for auto-transplantation therapy for CNS diseases"Restorative Neurology and Neuroscience. (In press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sasaki, M. et al.: "Transplantation of an acutely isolated bone marrow fraction repairs demyelinated adult rat spinal cord axons"Glia. 35. 26-34 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akiyama, Y. et al.: "Transplantation of clonal neural precursor cells derived from adult human brain establishes functional peripheral myelin in the rat spinal"Experimental Neurology. 167. 27-39 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 佐々木 祐典: "[細胞治療-再生医療への展開」神経幹細胞移植"最新医学. 2794 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Honmou, O., Sasaki, M., Oka, S., Takahashi, A., Tanooka, A., Uede, T., Hashi, K.: "A cell therapy for the CNS diseases"Restorative Neurology and Neuroscience. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takahashi, A., Honmou, O., Sasaki, M., Oka, S., Uede, T., Hashi, K.: "Transplantation of human neural stem cells into the ischemic lesion of the rat brain"Restorative Neurology and Neuroscience.. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Honmou, O., Uede, T., Hashi, K.: "Neural stem cells derived from adult human brain: implications for a cell therapy for CNS diseases."No Shinkei Geka. 29. 293-304 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Honmou, O., Sasaki, M., Akiyama, Y., Uede, T., Hashi, K.: "Clonal neural stem cells derived from adult human brain: Implication s for auto-transplantation therapy for CLS disease"Restorative Neurology and Neuroscience.. 17. 147 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sasaki, M., Honmou, O., Akiyama, Y., Uede, U., Hashi, K., and Kocsis, J. D: "Transplantation of an acutely isolated bone marrow fraction repairs demyelinated adult rat spinal cord axons"Glia.. 35. 26-34 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akiyama, Y., Honmou, O., Kato, T., Uede, T., Hashi, K. and Kocsis, J. D: "Transplantation of Clonal Neural Precursor Cells Derived from Adult Human Brain Establishes Functional Peripheral Myelin in the Rat spinal Cord"Experimental Neurology.. 167. 27-39 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sasaki, M., Honmou, O.: "A cell therapy - Neural regeneration"Advanced Meidicne. 2666-2670 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Honmou, O. et al.: "A cell therapy for the CNS diseases"Restorative Neurology and Neuroscience. (In press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takahashi, A. et al.: "Transplantation of human neural stem cells into the ischemic lesion of the rat brain"Restorative Neurology and Neuroscience. (In press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 本望 修: "神経幹細胞応用の可能性について(総説)"脳神経外科. 29(4). 293-304 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Honmou, O. et al.: "Clonal neural stem cells derived from adult human brain : Implications for auto-transplantation therapy for CNS diseases"Restrative neurology and Neuroscience. (In press). (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sasaki, M. et al.: "Transplantation of an acutely isolated bone marrow fraction repairs demyelinated adult rat spinal cord axons"Glia. 35. 26-34 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Akiyama, Y. et al.: "Transplantation of clonal neural precursor cells derived from adult human brain establishes functional peripheral myelin in the rat spinal cord"Experimental Neurology. 167. 27-39 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 佐々木 祐典: "「神経治療-再生医療への展開」神経幹細胞移植"最新医学. 2794 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 本望修 ら: "神経幹細胞応用の可能性について(総説)"脳神経外科. 29(4)(In press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Akiyama,Y. et al.: "Transplantation of clonal neural precursor cells derived from adult human brain establishes functional peripheral myelin in the rat spinal cord"Experimental Neurology. 167. 27-39 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Honmou,O. et al.: "Clonal neural stem cells derived from adult human brain : Implications for auto-transplantation therapy for CNS diseases.."Restorative Neurology and Neuroscience. (In press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sasaki,M. et al.: "Excitatory and inhibitory responses and synaptic transmission differentiated from the adult human neural stem cells in vitro"Restorative Neurology and Neuroscience. (In press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kato,T. et al.: "Transplantation of human olfactory ensheathing cells elicits remyelination of demyelinated rat spinal cord"Glia. 30. 209-218 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Honmou,O. et al.: "Transplantation of human neural stem cells repairs the injured central nervous system in the adult rat"Restorative Neurology and Neuroscience. 16. 15 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 本望修 ら: "脳の科学"神経疾患の神経幹細胞治療-動物モデルでの試み-. 127-132 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 端和夫 ら: "脳と循環"脳機能再生を目指した基礎的研究の現状と展望. 53-57 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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