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Development of a new surgical strategy in tumor resection for malignant bone and soft tissue tumors with cytocidal effect of acridine orange excited by various dynamic energy-wave.

Research Project

Project/Area Number 12470312
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionKyoto prefectural university of medicine

Principal Investigator

KUSUZAKI Katsuyuki  the medical department, Kyoto prefectural university of medicine lecturer, 医学部, 講師 (30177993)

Co-Investigator(Kenkyū-buntansha) URATA Yoji  the medical department, Kyoto prefectural university of medicine assistant professor, 医学部, 助教授 (30143944)
ASHIHARA Tsukasa  the medical department, Kyoto prefectural university of medicine professor (30079719)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥10,500,000 (Direct Cost: ¥10,500,000)
Fiscal Year 2001: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 2000: ¥5,100,000 (Direct Cost: ¥5,100,000)
Keywordsacridine orange / dynamic energy / photodynamic therapy / radiodynamic therapy / osteosarcoma / cytocidal effect / 電磁波 / 超音波
Research Abstract

In this study, we have undertaken to develop a new surgical strategy in tumor resection for malignant bone and soft tissue tumors, using cytocidal effect by acridine orange (AO) excited by various dynamic energy waves such as light-beam, X-ray, electro-magnetic field or ultra-sound. In vitro study using mouse osteosarcoma cell line revealed that AO excited with blue light beam (466 nm) or low-dose X-ray (5 Gy) showed a strong cytocidal effect, although both of an electro-magnetic field stimulation and ultra-sound did not. AO excited with blue light or X-ray was also effective to inhibit tumor growth in vivo study using mouse osteosarcoma inoculated subcutaneously. The mechanism of such a strong cytocidal effect of AO is speculated that intracellular free-radical as singlet oxygen which subsequently occurred after AO excitation with blue light or X-ray may kill tumor cells. We also found that AO has a biological feature to specifically accumulate into malignant tumor cells, especially, malignant bone and soft tissue tumors. Based on these results, at present, we clinically apply photodynamic therapy and radiodynamic therapy with AO to marginal or intra-lesional resection of malignant bone and soft tissue tumors.

Report

(2 results)
  • 2001 Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Kusuzaki Katsuyuki: "Photodynamic inactivation with acridine orange on a multidrug-resistant mouse osteosarcoma cell line"Jpn J Cancer Res. 91. 439-445 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kusuzaki Katsuyuki: "Polyploidization induced by acridine orange in mouse osteosarcoma cells"Anticancer Res. 20(2A). 965-970 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kusuzaki Katsuyuki: "Intracellular binding sites of acridine orange in living osteosarcoma cells"Anticancer Res. 20(2A). 971-976 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kusuzaki Katsuyuki: "Total tumor cell elimination with minimum damage to normal tissues in musculoskeletal sarcomas by photodynamic reaction with acridine orange"Oncology. 59(2). 174-180 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kusuzaki Katsuyuki: "Binuclear cells induced by acridine orange in giant cell tumor of hone"Anticancer Res. 20(5A). 3013-3018 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kusuzaki Katsuyuki: "Fluorovisualization effect of acridine orange on mouse osteosarcoma"Anticancer Res. 20(5A). 3019-3024 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirata Masazumi: "Drug resistance modification using pulsing electromagnetic field stimulation for multidrug resistant mouse osteosarcoma cell line"Anticancer Res. 21. 317-320 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kusuzaki Katsuyuki: "Acridine orange induced binucleation in chondrocytes"Osteoarthritis Cartilage. 9. 147-151 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hashiguchi Shin: "Acridin orange excited by low-dose radiation has a strong cytocidal effect on mouse osteosarcoma"Oncology. 62. 85-93 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kusuzaki K., Minami G., Takeshita H., Murata H., Hashiguchi S., Nozaki T., Ashihara T., Hirasawa: "Photodynamic inactivation with acridine orange on a multidrug-resistant mouse osteosarcoma cell line"Jpn J Cancer Res. 91. 439-445 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kusuzaki K., Takeshita H., Murata H., Gebhardt MC., Springfield DS., Mankin HJ., Ashihara T., Hirasawa: "Polyploidization induced by acridine orange in mouse osteosarcoma cells"Anticancer Res. 20 (2A). 965-970 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kusuzaki K., Murata H., Takeshita H., Hashiguchi S. Nozaki T., Emoto K., Ashihara T., Hirasawa Y.: "Intracellular binding sites of acridine orange in living osteosarcoma cells"Anticancer Res. 20 (2A). 971-976 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kusuzaki K., Aomori K., Suginoshita T., Minami G., Takeshita H., Murata H., Hashiguchi S., Ashihara T., Hirasawa Y.: "Total tumor cell elimination with minimum damage to normal tissues in musculoskeletal sarcomas by photodynamic reaction with acridine orange"Oncology. 59 (2). 174-180 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kusuzaki K., Takeshita H., Murata H., Hashiguchi S., Nozaki T., Emoto K., Ashihara T., Hirasawa Y.: "Binuclear cells induced by acridine orange in giant cell tumor of bone"Anticancer Res. 20 (5A). 3013-3018 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kusuzaki K, Suginoshita T, Minami G, Aomori K, Takeshita H, Murata H, Hashiguchi S, Ashihara T, Hirasawa Y.: "Fluorovisualization effect of acridine orange on mouse osteosarcoma"Anticancer Res. 20 (5A). 3019-3024 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirata M., Kusuzaki K., Takeshita H., Hashiguchi S., Mirata H., Hirasawa Y., Ashihara T.: "Drug resistance modification using pulsing electromagnetic field stimulation for multidrug resistant mouse osteosarcoma cell line"Anticancer Res. 21. 317-320 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kusuzaki K., Takeshita H., Murata H., Hashiguchi S., Nozaki T., Emoto K., Ashihara T., Hirasawa Y.: "Acridine orange induced binucleation in chondrocytes"Osteoarthritis Cartilage. 9. 147-151 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Hashiguchi, K. Kusuzaki, H. Murata, H. Takeshita, M. Hashida, T. Nishimura, T. Ashihara, Y. Hirasawa: "Acridine orange excited by low-dose radiation has a strong cytocidal effect on mouse osteosarcoma"Oncology. 62. 85-93 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kusuzaki K.,Takeshita H.,Murata H.,Hashiguchi S.,Nozaki T.,Emoto K.,Ashihara T.,Hirasawa Y: "Intracellular binding sites of acridine orange in living osteosarcoma cells."Anticancer Research. 20. 971-976 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kusuzaki K.,Minami G.,Takeshita H.,Murata H.,Hashiguchi S.,Nozaki T.,Ashihara T.,Hirasawa Y: "Photodynamic inactivation with acridine orange on a multi-drug resistant mouse osteosarcoma cell line."Jpn J Cancer Research. 91. 439-445 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kusuzaki K.,Aonnori K.,Suginoshitau T.,Minami G.,Takeshita H.,Murata H.,Hashiguchi S.,Ashihara T.,Hirasawa Y.: "Total tumor cell elimination with minimum damage to normal tissues in musculoskeletal sarcomas by photodynamic reaction with acridine orange."Oncology. 59. 174-180 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kusuzaki K.,Suginoshita T.,Minami G.,Aomori K.,Takeshita H.,Murata H.,Hashiguchi S.,Ashihara T.Hirasawa Y.: "Fluorovisualization effect of acridine orange on mouse osteosarcoma."Anticancer Research. 20. 3019-3024 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kusuzaki K.,Takeshita H.,Murata H.,Gebhardt M.C.,Springfield D.S.,Mankin H.J.,Ashihara T.,Hirasawa Y: "Polyploidization induced by acridine orange in mouse osteosarcoma cells."Anticancer Research. 20. 965-970 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 楠崎克之,橋口津,杉之下武彦,南銀次郎,青盛克裕,平澤泰介: "アクリジンオレンジを用いた悪性骨軟部腫瘍の完全腫瘍細胞除去のアイデア-より高い患肢機能を目指して-."整形・災害外科. 43(3). 271-277 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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