| Project/Area Number |
12470321
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
YUGE Osafumi Hiroshima University, Medical and Dental Hospital, Professor, 医学部・歯学部附属病院, 教授 (40034128)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUDA Hideki Hiroshima University, Medical and Dental Hospital, Research Associate, 医学部・歯学部附属病院, 助手 (00294588)
NIINAI Hiroshi Hiroshima University, Medical and Dental Hospital, Assistant Professor, 医学部・歯学部附属病院, 講師 (50208112)
KAWAMOTO Masashi Hiroshima University, Graduated of Biomedical Science, Associate Professor, 大学院・医歯薬学総合研究科, 助教授 (40127642)
前原 康宏 広島大学, 医学部・附属病院, 講師 (20238877)
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| Project Period (FY) |
2000 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥11,600,000 (Direct Cost: ¥11,600,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2000: ¥6,500,000 (Direct Cost: ¥6,500,000)
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| Keywords | Malignant Hyperthermia / Skeletal muscle / Ca-induced Ca release / Cell culture / Calcium homeostasis |
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| Research Abstract |
We measured the rate of CICR (Ca-induced Ca release) in 65 patients who had anesthesia-related malignant hyperthermia(MH) history, MH family history or diseases associated with MH. There were 18 patients with accelerated CICR rate and 47 patients with normal CICR rate. We succeeded to culture myoblast from 41 muscle strips. We studied the effect of caffeine on the calcium concentration in human myotubes made up of cultured skeletal muscle cells from 4 patients of accelerated CICR rate and 6 patients of normal CICR rate. The caffeine induced increase in calcium concentration of accelerated CICR rate group was significantly higher than normal CICR rate group. We studied the effect of halothane on the calcium concentration in human myotubes made up of cultured skeletal muscle cells from 2 patients of accelerated CICR rate and 4 patients of normal CICR rate. There was no significant difference of halothane-induced calcium increase between these two groups, because we guessed that 6 subjects was so few. We made genetic analysis of six mutations of the ryanodine receptor (RYR1) in 8 patients, but we didn't find out any mutations of the RYR1. We need much time and cost to search MH-linked mutations of RYR1, because RYR1 consists of 5000 amino acids and A7H related mutations on RYTR1 were reported almost 40 points.
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