MECHANISM OF REGULATION OF TELOMERASE EXPRESSION, AND EXPERIMENTAL STUDY ON CANCER GENE THERAPY USING ITS PROMOTER.
| Project/Area Number |
12470330
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kanazawa University |
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Principal Investigator |
NAMIKI Mikio Kanazawa Univ. Graduate School of Medical Science, Professor, 大学院・医学系研究科, 教授 (70155985)
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| Co-Investigator(Kenkyū-buntansha) |
KYO Satoshi Kanazawa Univ. Graduate School of Medical Science, Assistant Professor, 大学院・医学系研究科, 講師 (50272969)
KOH Eitetsu Kanazawa Univ. Hospital, Assistant Professor, 医学部・附属病院, 講師 (90283134)
KOSHIDA Kiyoshi Kanazawa Univ. Graduate School of Medical Science, Associate Professor, 大学院・医学系研究科, 助教授 (70186667)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥10,600,000 (Direct Cost: ¥10,600,000)
Fiscal Year 2001: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2000: ¥8,100,000 (Direct Cost: ¥8,100,000)
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| Keywords | telomerase / genetherapy / prostate cancer / CD gene / androgen / プロモーター / アンドログン / 細胞周期 |
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| Research Abstract |
Human telomerase reverse transcriptase (hTERT) is the catalytic subunit of telomerase, which is highly active in immortalized cells. We herein carried out basic experiments on cancer gene therapy utilizing the hTERT promoter. The core promoter region was identified by deletion analysis, and demethylation of this region accelerated the expression of p16 and E-cadherin. Cytosine deaminase gene was ligated in downstream of hTERT promoter. In vitro experiments demonstrated that cell- kill effect was only seen in cancer cells not in normal cells. Tandem repeat promoter is now under construction for creating more active and specific tool in gene therapy. Adenovirus vector containing hTERT promoter and CD was constructed, and used in vivo experiments. The data was promising and could be widely utilized in the field of cancer gene therapy because of its specificity.
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Report
(3 results)
Research Products
(13 results)
