| Project/Area Number |
12470383
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | NIIGATA UNIVERSITY |
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Principal Investigator |
HOSHINO Etsuro NIIGATA UNIVERSITY graduate school of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (90124619)
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| Co-Investigator(Kenkyū-buntansha) |
UEMATSU Hiroyuki graduate school of Medical and Dental Sciences, Assistant, 大学院・医歯学総合研究科, 助手 (40242432)
SATO Naomi graduate school of Medical and Dental Sciences, Assistant, 大学院・医歯学総合研究科, 助手 (20018427)
NAKAZAWA Futoshi graduate school of Medical and Dental Sciences, Associate Professor, 大学院・医歯学総合研究科, 助教授 (60115053)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2001: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2000: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | immune system / biofilm / actinomycosis / capusulation / neutorophils / 国際研究者交流 / 多国籍 / Actinomyces israelii / Actinomycosis / 被膜 / Actinomyces / 弱抗酸性菌 / 根尖病巣 |
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| Research Abstract |
The present research aimed to clarify the association of capsulation of bacterial masses with bacterial biofilm formation in actinomycotic and similar lesions, as a new host immune reaction.
1. Substances of capsules : Eosinophilic amorphous plastic-like structure surrounding bacteria masses in actinomycosis lesions was found to contained DNA and proteineous contents of neutrophils using fluorescent labeling techniques in vitro. Such bacteria in lesions were often stained to be weak acid-fast, depending on the amount of capsular substances. The bacterial masses in actinomycotic lesions are, thus, separated and isolated by the capsulation, which may reveal the characteristics of actinomycotic symptoms.
2. Capsulation in periapical lesions : Weekly acid-fast bacteria, including Actinomyces israelii and the similar types of bacteria such as Asaccharolytic Anaerobic Gram-positive Rods : AAGPR, were often found in periapical lesions collected in Asian countries including Japan. Tight biofilm capsulation may cause worse prognosis in endodontic treatment.
3. 3Mix-MP to treat the lesions with capsulated bacteria masses. A combination of 3 anti-bacteria drugs (3Mix) is efficient against all the types of oral bacteria. Propylene glycol (P in MP) makes it possible to carry 3Mix through the capsules efficiently and kill bacteria inside.
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