| Project/Area Number |
12470385
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
NAGAI Noriyuki Okayama University, Graduate School of Medicine and dentistry, Oral pathology and Medicine, Professor, 大学院・医歯学総合研究科, 教授 (90085770)
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| Co-Investigator(Kenkyū-buntansha) |
GUNDUZ Mehmet Okayama University, Graduate School of Medicine and dentistry, Oral pathology and Medicine, Assistant, 大学院・医歯学総合研究科, 助手 (70333507)
TSUJIGIWA Hidetsugu Okayama University, Graduate School of Medicine and dentistry, Oral pathology and Medicine, Assistant, 大学院・医歯学総合研究科, 助手 (70335628)
NAGATSUKA Hitoshi Okayama University, Graduate School of Medicine and dentistry, Oral pathology and Medicine, Associate Professor, 大学院・医歯学総合研究科, 助教授 (70237535)
KOMORI Hirofumi Osaka University, Graduate School of Medicine, Faculty of Medicine, Assistant, 大学院・医学系研究科, 助手 (00252677)
NAGAOKA Noriyuki Okayama University, School of Dentistry, Teaching Assistant, 大学院・医歯学総合研究科, 教授 (70304326)
中野 敬介 岡山大学, 歯学部, 助手 (10325095)
石割 裕三 岡山大学, 歯学部, 助手 (10304312)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 2002: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2000: ¥6,800,000 (Direct Cost: ¥6,800,000)
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| Keywords | Tooth Germ / BMP / Cbfa1 / Odontogenic Tumor / Basement Membrane / Type IV Collagen / 成長因子 / Cbfal |
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| Research Abstract |
To clarify the reciprocal roles of epithelium and mesenchyme in tooth germ differentiation process, we investigated the localization and change of BMP family gene expression and each alpha chain of collagen IV, which is a main basal membrane component, in tooth germ development. The expressions of BMP-4 and BMP-7 at fetal age 11^<th> day and BMP-2 expression at fetal age 12^<th> day were shown. Moreover, each of alpha chains of collagen IV was localized in basal membrane of tooth germ epithelium in a stage dependent manner.
We examined the role of BMP family to understand the odontogenic tumor cell differentiation. In ameloblastoma, amelogenin expression was detected in tumor cells with a morphologic similarity to early tooth germ. In terms of functional differentiation, amelogenin was expressed in late differentiation stage. In desmoplastic ameloblastoma, BMP family was shown to have a possible role for the differentiation of stroma into periodontal tissue. In adenomatoid odontogenic tumor, BMP family localization was resembled to matrix formation stage of tooth germ. In summary, we showed the special role of BMP family in induction and differentiation of each tissue type into parenchyma and stroma in odontogenic tumors.
Then we analyzed the role of cbfal in matrix protein expression and bone formation in mouse tooth germ and showed its role in matrix protein expression and morphologic formation of tooth germ. Furthermore, cbaf1 knock-out mouse studies demonstrated that differentiation and transcription regulation in incisor tooth germ by cbfa was different than in osteoblasts.
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