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Molecular mechanisms for expression of receptor and its related molecules in taste cells and selective synaptic formation between corresponding classes of taste cells and axons

Research Project

Project/Area Number 12470394
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionKyushu University

Principal Investigator

NINOMIYA Yuzo  Kyushu University, faculty of Dental Sciences, Professor, 大学院・歯学研究院, 教授 (50076048)

Co-Investigator(Kenkyū-buntansha) SHIGEMURA Noriatsu  Kyushu University, Faculty of Dental Sciences, Research Associate, 大学院・歯学研究院, 助手 (40336079)
MISHIMA Kazyo  Kyushu University, Faculty of Dental Science, Research Associate, 大学院・歯学研究院, 助手 (50117256)
SASAMOTO Kazushige  Kyushu University, Faculty of Dental Sciences, Associate Professor, 大学院・歯学研究院, 助教授 (30128029)
ISHIZUKA Satoru  Kyushu Institute of Technology, Faculty of Technology, Associate Professor, 工学部, 助教授 (40124804)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥11,800,000 (Direct Cost: ¥11,800,000)
Fiscal Year 2002: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2001: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2000: ¥4,600,000 (Direct Cost: ¥4,600,000)
KeywordsSynapse formation / sweet receptor / Leptin receptor / Gurmarin-sensitive receptor / db mouse
Research Abstract

The purpose of this study is to investigate mechanisms for genetic induction of selective synaptic formation between taste cells and axons and expression of receptor and its related molecules in taste cells by using mouse strains possessing combinations of sweet taste receptors (SwR), such as GS-SwR or GISwR, and nor mal and mutated leptin receptors (ObRs). The results lndicated that 1)among several different isoforms of both Ob-Rs and STATs (signal transduction related factors), Ob-Rb as the leptin receptor and STAT3 as the transcription factor would be most active and functional forms in taste cells. 2)the fact that strain with a conbination of mutated Ob-Rb and GI-SwR showed enhanced sweet preference and responses suggested that leptin modulation on sweet taste may be through activation of taste cells possessing the GI-SwR. 3)In mice with crush of the chorda tympani nerve, responses of regenerated nerve to sweet substances were recognized at around 3 weeks after crush. At this period GS was found in some of animals indicating early start recovery of GS-SwR. Reappearance of GS was varied among sweeteners, suggesting multiple sweet receptor systems even in GS-SwR.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Sugimoto K., et al.: "Ion channels and second messengers involved in transduction and modulation of sweet taste in mouse taste cells"Pure Appl.Chem.. 74. 1141-1151 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Lindemann B., Ogiwara Y., Ninomiya Y.: "The discovery of umami"Chem.Senses. 27. 843-844 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kusakabe Y., et al.: "The neural differentiation gene Mash-1 has a distinct pattern of expression from the taste reception^related genes gustducin"Chem Senses. 27. 445-451 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ninomiya Y., et al.: "Leptin and sweet taste"Vatam.Horm.. 64. 221-248 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishimuta K., Sasamoto K., Ninomiya Y.: "Neural activities in the substantia nigra modulated by stimulation of the orofacial motor cortex and rhythmical jaw movements"Neuroscience. 113. 915-923 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ninomiya, Y., Shiiemura, N., Yasumatsu, K., Ohta, R., Sugimoto, K., Nakashima, K., Lindeman, B.: "Leption and Sweet Taste"Vitam.Horm.. 64. 221-248 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kusakabe, Y., Miura, H., Hashimoto, R., Sugiyama, C., Ninomiya, Y., Hino, A.: "The Neural Differentiation Gene Mashi-1 has a Distinct Pattern of Expression from the Taste Reception-related Genes gustducin and T1R2 in the Taste Buds"Chem.Senses. 27. 445-451 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Lindemann, B., Ogiwara, Y., Ninomiya, Y.: "The Discovery of Umami"Chem.Senses. 27. 843-844 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sugimoto, K., Shigemura, N., Yasumatsu, K., Ohta, R., Nakashima, K., Ninomiya Y.: "Ion channels and second messengers involved in transduction and modulation of sweet taste In mouse taste cells. Participation in the suppressive modulation of sweet sensitivity by leptin"Pure Appl.Chem. 74. 1141-1151 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishimuta, K., Sasamoto, K., Ninomiya, Y.: "Neural activities in the substantia nigra modulated by stimulation of the orofacial motor cortex and rhythmical jaw movements in the rat"Neuroscience. 113. 915-923 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Katsukawa, H. et al.: "Salivary cystatins influence ingestion of capsaicin-containing diets in the rat"Life Sciences. 71. 457-467 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kusakabe, Y. et al.: "The Neural Differentiation Gene Mashi-1 has a Distinct Pattern of Expression from the Taste Reception-related Genes gustducin and T1R2 in the Taste Buds"Chem. Senses. 27. 445-451 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Lindemann, B. et al.: "The Discovery of Umami"Chem. Senses. 27. 843-844 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sugimoto, K. et al.: "Ion channels and second mesengers involved in transduction and modulation of sweet taste in mouse taste cells. Participation in the suppressive modulation of sweet sensitivity by leptin"Pure Appl. Chem. 74. 1141-1151 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nishimuta, K. et al.: "Neural activities in the substantia nigra modulated by stimulation of the orofacial motor cortex and rhythmical jaw movements in the rat"Neuroscience. 113. 915-923 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 安松啓子: "味を感じる:神経メカニズム"細胞工学. 21. 1429-1433 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ninomiya, Y. et al.: "Leptin and sweet taste"Vitam. Horm.. 64. 221-241 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sasamoto, K. et al.: "Analysis of rhythmical jaw movements produced by taste stimulation in rats"J. Nutr. Sci. and Vitaminol.. 47・2. 156-160 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakashima, K. et al.: "Behavioral taste similarities and differences among monosodium L-glutamate and glutamate receptor agonists in C57BL mice"J. Nutr. Sci. and Vitaminol.. 47・2. 161-166 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Miura, K. et al.: "Shh and Ptc are associated with taste bud maintenance in the adult mouse"Mechanisms of Develop, emt. 106. 143-145 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kitagawa, K. et al.: "Molecular Genetic Identification of a Candidate Receptor Gene for Sweet Taste"Biochemical and Biophysical Research Communications. 283. 236-242 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Imoto, S. et al.: "β-Cyclodextrin inhibits the sweet taste suppressing activity of gurmarin by the formation of an inclusion complex with aromatic residues in gurmarin"Canadian Journal of Physiology and Pharmacology. 79. 836-840 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ninomiya,Y. et al.: "Responses to umami substances in taste bud cells innervated by the chorda tympani and glossopharyngeal nerves 1,2."J.Nutr.. 130. 950S-953S (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kawai,K. et al.: "Leptin as a modulator of sweet taste sensitivities in mice."Proc.Natl.Acad.Sci.USA. 97(20). 11044-11049 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Li,X. et al.: "High-resolution genetic mapping of the saccharin preference locus (Sac) and the putative sweet taste receptor (T1R1) gene (Gpr70) to mouse distal chromosome 4."Mamm.Genome.. 12. 13-16 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ninomiya,Y. et al.: "Leptin and sweet taste."Vitam.Horm.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sasamoto,K. et al.: "Analysis of rhythmical jaw movements produced by taste stimulation in rats."J.Nutr.Sci.and Vitaminol.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakashima,K. et al.: "Behavioral taste similarities and differences among monosodium L-glutamate and glutamate receptor agonists in C57BL mice."J.Nutr.Sci.and Vitaminol.. (in press). (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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