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Analysis of Hellcobacter pylori infection route from oral biological view point.

Research Project

Project/Area Number 12470400
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 病態科学系歯学(含放射線系歯学)
Research InstitutionTOKYO DENTAL COLLEGE

Principal Investigator

ISHIHARA Kazuyuki  TOKYO DENTAL COLLEGE, DEPARTMENT OF DENTISTRY, ASSOCIATE PROFESSOR, 歯学部, 助教授 (00212910)

Co-Investigator(Kenkyū-buntansha) OKUDA Katsuji  TOKYO DENTAL COLLEGE, DEPARTMENT OF DENTISTRY, PROFESSOR, 歯学部, 教授 (40085741)
MIURA Tadashi  TOKYO DENTAL COLLEGE, DEPARTMENT OF DENTISTRY, ASSOCIATE PROFESSOR, 歯学部, 助教授 (10266570)
NISHIDA Jiro  TOKYO DENTAL COLLEGE, DEPARTMENT OF DENTISTRY, PROFESSOR, 歯学部, 教授 (50198470)
北洞 哲治  国立大蔵病院, 消化器科, 医長
久松 理一  東京歯科大学, 歯学部, 助手 (60255437)
Project Period (FY) 2000 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 2003: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2002: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsHelicobacter pylori / Campylobacter rectus / Oral cavity / Heat shock protein / Colonization / Adherence / Shared antigen / Peridontitis / Periodontitis / Cross reactivity / Coaggregation / Periodonititis / 感染経路 / 診断 / 交差反応性 / 歯周病原細菌 / 唾液 / 口腔 / 口腔感染症 / 共通抗原 / 歯周病原菌
Research Abstract

Increasing evidence has linked colonization by Helicobacterpylori with the development of gastric and peptic ulcer. In this study, a role of oral microorganisms on H. pylori colonization and a reactivity of IgG and IgA elicited by Cainpylorbacter nectus and H.pylori were examined.
Streptococcus oralis, Streptococcus mutans, and Prevotella intermedia inhibited the growth of H.pylon. Especially, no viable H.pylori cells were detected after co-incubation of H. pylori and P.intermedia for 24 hours (Ref.1). This result indicated that H. pylori may not grow in dental plaque. In addition, we observed that 50% of H.pylori transferred to coccoid form (uncultivable form) after exposure to P.intermedia or S.mutans (Ref.2).
To clarify a role of immune response elicited by C.rectus against H.pylori infection, we evaluated a titer of IgG and IgA in saliva and serum from patients with gastric-and duodenal ulcer. Antibody titers of salivary IgG and IgA against H.pylori and C.rectus correlated each other. Analysis of immunoblotting indicated that rabbit anti-H.pylori antibody reacted to proteins of C.rectus and that rabbit anti-C.rectus antibody recognized H. pylori proteins (Ref.2). A antibody against H.pylori HSP reacted C.rectus protein with similar molecular mass of H.pylori HSP (Ref.3). It is possible that the immunoresponse elicited to C.rectus and H.pylori are associated with other systemic disease. Increase of antibody level against HSP of Chiamydia pneurnoniae was suggested as a risk factor of atherosclerosis (Chem et al. 2003, FEMS Innunol.Med.Micribiol.36: 187-192). Taking these data together, antibody against HSP induced H.pylori and C.rectus possibly associated with atherosclerosis formation (Ref.4).
In conclusion, we clarified that the H. pylon attach to oral cavity but does not colonize in it. C.rectus infection elicited antibody react to H. pylori and it might influence on host defense against H.pylori.

Report

(5 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Okuda, K. et al.: "Involvement of periodontopathic biofilm in vascular diseases"Oral Dis.. 10・1. 5-12 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okuda, K. et al.: "Ecological and immunopathological implications of oral bacteria in Helicobacter pylori-infected disease"J.Periodontol.. 74・1. 123-128 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishihara, K., et al.: "Shared antigenicity between Helicobacter pylori and periodontopathic Campylobacter rectus strains"FEMS Microbiol.Lett.. 197・1. 23-27 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okuda, K. et al.: "Helicobacterpylori may only a transient presence in the oral cavity and on the surafece of oral cancer"Microbiol.Immunol.. 44・5. 385-388 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okuda, K., Ishihara, K., Miura, T., Katakura, A.: "Helicobacter pylori may have only a transient presence in the oral cavity and on the surface of oral cancer"Microbiol.Immunol.. 44-5. 385-388 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ishihara, K., MIURA, T., EBLHARA, Y., HIRAYAMA.T., KAMIYA, S., OKUDA., K.: "Shared antigenicity between Helicobacter pylori and periodontopathic Campylobacter rectus strains"FEMS Microbiol.Lett.. 197-191. 23-27 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] OKUDA, K., KIMIZUKA, R., KATAKURA, A., NAKAGAWA, T., ISHIHARA, K.: "Ecological and immunopathological implications of oral bacteria in Helicobacter pylon-infected disease"J.Periodontol.. 74-71. 123-128 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] OKUDA, K., KATO, T., ISHIHARA, K.: "Involvement of periodontopathic biofilm in vascular diseases"Oral Dis.. 10-1. 5-12 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Okuda, K. et al.: "Ecological and immunopathological implications of oral bacteria in Helicobacter pylori-infected disease"J.Periodontol.. 74・1. 123-128 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ishihara, K. et al.: "A 43 kDa protein of Treponema denticola is essential for dentilisin activity"FEMS Microbiol.Lett.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ishihara, K. et al.: "Correlation between the Detection of Periodontopathic Bacterial DNA in Carotid Coronary Stenotic Artery Plaque and Dental Plaque Samples"J.Clin.Microbiol.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Inagaki, S. et al.: "Antibody responses of periodontitis patients to gingipains of Porphyromonas gingivalis"J.Periodontol.. 74・10. 1432-1439 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Asano, H. et al.: "Relationship between transmission of Porphyromonas gingivalis and fimA type in spouses"J.Periodontol.. 74・9. 1355-1366 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Eguchi, J. et al.: "PCR method is essential for detecting Mycobacterium tuberculosis in oral cavity samples"Oral Microbiol.Immunol.. 18・3. 156-159 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Adachi, M. et al.: "Effect of professional oral care on elderly living in nursing homes"Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology. 94・2. 191-195 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Chattin-Kacouris, B.R.et al.: "Heat schock protein of Mycoplasma saliarium and Mycoplasma orale strains isolated from HIV-seropositive patients"Bull Tokyo Dent Coll. 43. 231-236 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sumida, S. et al.: "Transmission of periodontal disease associated bacteria from teeth to osseointegrated implant regions"Int J Oral Maxillofac Implants. 17・5. 696-702 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ihara H. et al.: "Detection of Campylobacteer rectus in periodontitis sits by monoclonal antibody"J Periodontal Res.. 38・1. 64-72 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Okuda, M. et al.: "Reduction of potential respiratory pathogens by oral hygienic treatment in patients undergoing endotracheal anesthesia"J. Anesth.. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Okuda, K. et al.: "Ecological and immunopathological implication of oral bacteria in Helicobacter pylori infected disease"J. Periodontol.. 74・1. 123-128 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hirose, M., Ishihara, K., et al.: "Expression of cytokines and inducible nitric oxide synthase in inflamed gingival tissue"Journal of Periodontology. 72・5. 590-597 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Abe, S., Ishihara, K. et al.: "Prevalence of potential respiratory pathogens in the mouths of elderly patients and effects of professional oral care"Archives of Gerontology and Geriatrics. 32・1. 45-55 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yonezawa, H., Ishihara, K.: "An arggingipain A DNA vaccine induces protective immunity"Infection and immunity. 69・5. 2858-2864 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ishihara, K. et al.: "Shared antigenicity between Helicobacter pylori and periodontopathic Campylobacter rectus strains"FEMS Microbiology Letters. 197・1. 23-27 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 石原和幸: "Treponema denticolaの表層成分の病原性解析"日本細菌学会雑誌. 56・2. 413-420 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ishihara,K. et al.: "Relationships between the onset of pustulosis palmaris et plantaris, periodontitis, and bacterial heat shock proteins"Oral Microbiol.Immunol.. 15. 232-237 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Abe,S.,Ishihara,K. et al.: "Prevalence of potential respiratory pathogens in the mouths of elderly patients and effects of professional oral care"Arch.Gerontol.Geriatr.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ishihara,K. et al.: "Shared antigenicity between Helicobacter pylori and periodontopathic Campylobacter rectus Strains"FEMS Microbiol.Lett.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hirose,M.,Ishihara,K., et al.: "Expression of cytokines and inducible nitric oxide synthase in inflammed gingival tissue"J.Periodontol.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Okuda,K.,Ishihara,I. et al.: "Detection of Treponema denticola in atherosclerotic lesions"J.Clin.Microbiol.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yonezawa,H.,Ishihara,K. et al.: "An arggingipain A DNA vaccine induces protective immunity"Infect.Immun.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 石原和幸他分担 奥田克爾他編: "歯周病学最前線"日本歯科評論社. 192-196,278-283 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 奥田克爾,石原和幸他分担 岡田宏 他監修: "先端医療シリーズ・歯科医学2 歯周病-新しい治療を求めて"寺田国際事務所/先端医療技術研究所. 393-397 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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