The molecular-biological study of the mechanism of bone resorption controlled by periodontal ligament cells
| Project/Area Number |
12470409
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
TANI-ISHII Nobuyuki Kanagawa Dental College, Associate Professor, 歯学部, 講師 (20163610)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIKAWA Takashi Yokohama City University, Assistant Professor, 医学部, 助手 (80275049)
CHIEDA Keiko Kanagawa Dental College, Assistant Professor, 歯学部, 助手 (40267513)
MINAMIDA Genshi Kanagawa Dental College, Assistant Professor, 歯学部, 助手 (70288083)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2000: ¥12,000,000 (Direct Cost: ¥12,000,000)
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| Keywords | Osteoclast / Periodontal ligament cell / ODF / OPG / OCIF / BICORE / ODF / MC3T3-G2 / PA6 / BIOCORE |
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| Research Abstract |
Bone resorption is associated with loss of integrity f the periodontal ligament, followed by recruitment of resorptive cells. The purpose of this study was to investigate the mechanisms involved in the differentiation of osteoclast form bone marrow cells by periodontal ligament cells (MPDL). Experiments have been carried out to determine the osteoclast differentiation ability used in a co-culture of C57Black/6N mouse bone marrow cells and cloning MPDLs in vitro. Osteoclasts were indentified by being positive for tartrate-resistant acid phosphatase and with multi-nucleated cells (TRAP-positive cell) and bone resorbing ability assessed by pits formation on dentin slices. Several clomed cell lines were obtained from mouse periodontal ligament and screened for their ability to induce TRAP-positive cells in response to 1,25 dihydroxy-vitamin D3 and dexamethason in co-cultures with MPDLs. A cell line, MPDL-27 cells to produce this effect was great as same as that of bone marrow-derived stromal cells line (MC3T3-PA6). MPDL-27 expressed the cell adhesion molecules ICAM-1 and VCAM1. The expression of osteoprotegrin (OPG) and OPG ligand (osteoclast differentiation factor) of MPDL-27 was examined by reverse transcriptase-polymerase chain reaction, mRNA of OPG ligand and no mRNA of OPG had detected. These results indicated that MPDL have the ability to promote osteoclast differentiation by a contact-requiring process and may modulate the cascade of bone resorption.
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Report
(4 results)
Research Products
(1 results)
