| Co-Investigator(Kenkyū-buntansha) |
MATSUMURA Tatsushi Dental Hospital, Osaka University, Senior resident, 歯学部・附属病院, 医員
MORIYAMA Tomoji Graduate School of Dentistry, Osaka University, Res Assist, 大学院・歯学研究科, 助手 (90314385)
NAKAZAWA Mitsuhiro Dental Hospital, Osaka University, Assistant Professor, 歯学部・附属病院, 講師 (70217701)
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| Budget Amount *help |
¥13,800,000 (Direct Cost: ¥13,800,000)
Fiscal Year 2001: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 2000: ¥8,900,000 (Direct Cost: ¥8,900,000)
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| Research Abstract |
A high malignant squamous cell carcinoma(SCC), FI cell line has a potential to develop lung metastasis, regional lymph nodes metastasis ; extra-nodal spreads, hypercalcemia, leukocytosis and cachexia in nude mice as well as in the patient. A mouse bearing FI tumor developed cachexia and died within a month, because of its malignancy. Repeating several procedures which the cell line derived from lung metastases, the other cell from regional lymph nodes metastases are again inoculated subcutaneously to nude mice, we established L cell and N cell which are easy to develop metastases to lung and regional lymph nodes, respectively. Compared with characteristics of parental FI cell, morphologically, L cell and N cell have relatively loose cell-cell contact. L cell (W.B.C./Volume : 1 X 10^5/cm^3) developed leukocytosis more severe than that of FI cell (5 X 10^4/cm^3). Granulocyte-Colony Stimulating Factor (G-CSF) production (20 ng/ml for 48h) in the conditioned media(CM) of L cell were about
… More
50 times higher than those of FI cell. G-CSF production (1 ng/ml for 48h) in the CM of N cell were about 10 times higher than that of FI cell. As FI cell has G-CSF Receptor (G-CSFR) expression, the experiments related with FI cell and L cell proliferation used rG-CSF and anti-G-CSF neutralizing antibody, indicated that G-CSF might be weak autocrine growth factor for FI cell. Next, in patients with oral SCC we investigated whether G-CSF and G-CSFR expression were generally related with lung metastases or lymph nodes metastases or not. The results were as follows (1) Out of 106 oral SCCs, 56 cases(53 %) were positive for G-CSFR and 56 (53 %) were positive for G-CSF. (2) Expression of G-CSFR correlated significantly with Tumor size (P=0.0078), Stage of disease(P=0,0084), Death(P=0.012) and Expression of G-CSF(P=0.05), (3) Expression of G-CSF correlated significantly with Tumor size(P=0.039) and Expression of G-CSF(P=0.05). (4) None of them correlated with regional lymph nodes metastases nor lung metastases. 5-year-survival-rate was 78 % in G-CSFR-positive, 46 % in G-CSFR-negative, respectively and these are statistically significant.
These results indicated that expression of G-CSFR correlates with prognosis of patients with oral SCC. Less
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